Metabolic Fingerprinting and Profiling of Arabidopsis thaliana Leaf and its Cultured Cells T87 by GC/MS

Cell suspension cultures are now recognized as important model materials for plant bioscience and biotechnology. Very few studies of metabolic comparisons between cell cultures and original plants have been reported, even though the biological identity of cultured cells with the normally grown plant is of great importance. In this study, a comparison of the metabolome for primary metabolites extracted from the leaves of Arabidopsis thaliana and cultured cells from an Arabidopsis suspension culture (cell line T87) was performed. The results suggest that although cell suspension cultures and Arabidopsis leaves showed similarities in the common primary metabolite profile, nonetheless, moderate differences in quantitative profile were revealed.

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Plant Metabolism Of Xenobiotics. Comparison Of The Metabolism Of 3.4-Dichloroaniline In Soybean Excised Leaves And Soybean Cell Suspension Cultures

Zeitschrift für Naturforschung C ◽

10.1515/znc-1992-11-1207 ◽

1992 ◽

Vol 47 (11-12) ◽

pp. 823-829 ◽

Cited By ~ 15

Author(s):

Christine Gareis ◽

Christiane Rivero ◽

Ingolf Schuphan ◽

Burkhard Schmidt

Keyword(s):

Cell Suspension ◽

Cultured Cells ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Wall Structures ◽

End Products ◽

Glycine Max L ◽

Metabolism Of Xenobiotics ◽

Soybean Cell ◽

Detoxification Pathways

Excised leaves and cell suspension cultures of soybean (Glycine max L.) were incubated with [UL-14C]-3,4-dichloroaniline. The compound was almost completely metabolized after 48 h in both systems; it was apparent that the major detoxification pathways present in the excised leaves were also present in the cultured cells. Besides considerable amounts of insoluble residues, the N-glucosyl and the N-malonyl conjugates of 3,4-dichloroaniline, and a yet unknown metabolite was formed in the excised leaves; tentatively the latter was identified with the 6′-O-malonylester of N-glucosyl-3,4-dichloroaniline. The cell suspension cultures produced predominantly the N-malonyl conjugate, besides negligible amounts of the N-glucosyl conjugate, and insoluble residues; the majority of the N-malonyl compound was excreted into the medium. It was shown, that the metabolism of 3,4-dichloroaniline in excised leaves and cell suspension cultures of soybean was directed towards different end products: the excised leaves were able to make extensive use of cell wall structures as a deposit for xenobiotic bound residues, resembling plants in this respect; lack of these structures in the cultured soybean cells resulted in a soluble end product, namely the N-malonyl conjugate

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Ketoisophorone Transformation by Marchantia polymorpha and Nicotiana tabacum Cultured Cells

Zeitschrift für Naturforschung C ◽

10.1515/znc-2008-5-615 ◽

2008 ◽

Vol 63 (5-6) ◽

pp. 403-408 ◽

Cited By ~ 4

Author(s):

Mohamed-Elamir F. Hegazy ◽

Toshifumi Hirata ◽

Ahmed Abdel-Lateff ◽

Mohamed H. Abd El-Razek ◽

Abou El-Hamd H. Mohamed ◽

...

Keyword(s):

Nicotiana Tabacum ◽

Cell Suspension ◽

Cultured Cells ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Marchantia Polymorpha ◽

Minor Components

Stereospecific olefin (C=C) and carbonyl (C=O) reduction of the readily available prochiral compound ketoisophorone (2,2,6-trimethyl-2-cyclohexene-1,4-dione) (1) by Marchantia polymorpha and Nicotiana tabacum cell suspension cultures produce the chiral products (6R)-levodione (2), (4R,5S)-4-hydroxy-3,3,5-trimethylcyclohexanone (3), and (4R,6R)-actinol (4) as well as the minor components (4R)-hydroxyisophorone (5) and (4S)-phorenol (6).

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Comparative Investigations on the Metabolism of the Herbicide 2-(2,4-Dichlorophenoxy)-propionic Acid in Plants and Cultured Cells of Tomato

Zeitschrift für Naturforschung C ◽

10.1515/znc-1989-3-410 ◽

1989 ◽

Vol 44 (3-4) ◽

pp. 233-236 ◽

Cited By ~ 3

Author(s):

G. Bärenwald ◽

B. Schneider ◽

H. R. Schütte

Keyword(s):

Cell Suspension ◽

Propionic Acid ◽

Cultured Cells ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Aqueous Acetone ◽

Carbohydrate Components

Abstract Akademie der Wissenschaften der DDR , Institut für Biochemie der Pflanzen, Weinberg 3, Halle/Saale. The metabolism of [1-14C]2-(2,4-dichlorophenoxy)-propionic acid was studied in excised plants and cell suspension cultures of tomato. It was rapidly taken up and metabolized by both the plants and the cultured cells. The metabolites, isolated by extraction with aqueous acetone, separated and purified by TLC and HPLC, were identified by chemical and spectrometric methods. Conjugates with carbohydrates were detected. Glucose, diglucose as the main conjugating moiety, and triglucose were found as carbohydrate components within the conjugates. Almost the same conjugates occurred in plants and cultured cells.

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