Inactivation of Staphylococcal Enterotoxin-A with an Electrolyzed Anodic Solution

2002 ◽  
Vol 50 (1) ◽  
pp. 230-234 ◽  
Author(s):  
Tetsuya Suzuki ◽  
Jun Itakura ◽  
Masumi Watanabe ◽  
Mari Ohta ◽  
Yuri Sato ◽  
...  
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Anodic Solution

Immunofluorescence Assay Using Monoclonal and Polyclonal Antibodies for Detection of Staphylococcal Enterotoxins A in Milk

2019 ◽  
Vol 13 (1) ◽  
pp. 137-145 ◽  
Author(s):  
Tzonka Godjevargova ◽  
Zlatina Becheva ◽  
Yavor Ivanov ◽  
Andrey Tchorbanov
Keyword(s):  
Monoclonal Antibody ◽  
Magnetic Nanoparticles ◽  
Polyclonal Antibody ◽  
Polyclonal Antibodies ◽  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Fluorescence Immunoassay ◽  
Magnetic Separator ◽  
Milk Samples

Objectives: Staphylococcus aureus is a Gram-positive microorganism. S. aureus can grow in various foods and cause food poisoning by secreting enterotoxins. The most common enterotoxins involved in food poisoning are staphylococcal enterotoxin A and staphylococcal enterotoxin B, but Staphylococcal Enterotoxin A (SEA) is predominant. The main types of food contaminated with SEs are meat and meat products, poultry and eggs, milk and dairy products. The aim of this study was to develop a rapid and sensitive fluorescence immunoassay for detection of staphylococcal enterotoxin A in milk. Methods: Monoclonal and polyclonal antibodies for SEA were produced and characterized. Competitive fluorescence immunoassay based on Magnetic Nanoparticles (MNPs) was performed and optimized. MNPs were used as a solid carrier of the antibodies. The first step of the assay was immunoreaction between the immobilized antibody onto MNPs and SEA in milk sample. Then the fluorescein-SEA conjugate was added to the sample. Thus, competitive immunoreaction between MNP-mAb/MNP-pAb with SEA and SEA-FITC was performed. These immuno-complexes were separated by a magnetic separator and the obtained supernatants were analyzed. The fluorescent signal from the excess of conjugated SEA was proportional to the SEA contained in the milk. The assay duration was only 30 min. Results: The fluorescence immunoassays performed with polyclonal antibody had linear ranges from 5 pg/mL to 100 ng/mL SEA in a buffer, and from 50 pg/mL to 50 ng/mL SEA in spiked milk samples. While the same assays performed with monoclonal antibody had linear ranges from 1 pg/mL to 20 ng/mL SEA in buffer, and from 10 pg/mL to 10 ng/mL SEA in spiked milk samples. The detection limits of the developed immunoassays performed in milk were: 48 pg/mL with polyclonal antibody and 9 pg/mL with monoclonal antibody. Conclusion: A rapid and sensitive fluorescence immunoassay based on magnetic nanoparticles with a polyclonal and monoclonal antibody for determination of staphylococcal enterotoxin A in milk was developed.

Temperature Dependence of the Production of Staphylococcal Enterotoxin A byStaphylococcus aureus

2013 ◽  
Vol 77 (1) ◽  
pp. 30-37 ◽  
Author(s):  
Satomi TSUTSUURA ◽  
Yuko SHIMAMURA ◽  
Masatsune MURATA
Keyword(s):  
Temperature Dependence ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A

scholarly journals Mass Outbreak of Food Poisoning Disease Caused by Small Amounts of Staphylococcal Enterotoxins A and H

2005 ◽  
Vol 71 (5) ◽  
pp. 2793-2795 ◽  
Author(s):  
Tetsuya Ikeda ◽  
Naoto Tamate ◽  
Keiji Yamaguchi ◽  
Sou-ichi Makino
Keyword(s):  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Raw Material ◽  
Staphylococcal Enterotoxin A ◽  
Staphylococcal Enterotoxins ◽  
Reconstituted Milk

ABSTRACT It was believed that food poisoning in Osaka in 2000 was due to small amounts of staphylococcal enterotoxin A (SEA) in reconstituted milk. Results of this study clearly indicate that SEH was also present in the raw material of reconstituted milk, indicating that the food poisoning was caused by multiple staphylococcal enterotoxins.

scholarly journals Production of Staphylococcal Enterotoxin A in Blue, Brick, Mozzarella, and Swiss Cheeses

1973 ◽  
Vol 56 (4) ◽  
pp. 429-435 ◽  
Author(s):  
S.R. Tatini ◽  
W.D. Wesala ◽  
J.J. Jezeski ◽  
H.A. Morris
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A

Fluorescent Immunoassay for Determination of Staphylococcal Enterotoxin A in Milk by Immobilized F(ab')2 Fragment of Anti-enterotoxin A Monoclonal Antibody

2021 ◽  
Author(s):  
Z. R. Becheva ◽  
Y. L. Ivanov ◽  
N. M. Mihaylova ◽  
I. K. Manoylov ◽  
T. I. Godjevargova
Keyword(s):  
Monoclonal Antibody ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A

Rapid immunofluorescence assay for staphylococcal enterotoxin A using magnetic nanoparticles

2018 ◽  
Vol 54 (3) ◽  
pp. 916-922 ◽  
Author(s):  
Zlatina Becheva ◽  
Yavor Ivanov ◽  
Katya Gabrovska ◽  
Tzonka Godjevargova
Keyword(s):  
Magnetic Nanoparticles ◽  
Immunofluorescence Assay ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A

scholarly journals Establishment of Highly Specific and Quantitative Immunoassay Systems for Staphylococcal Enterotoxin A, B, and C Using Newly-Developed Monoclonal Antibodies

2005 ◽  
Vol 49 (7) ◽  
pp. 589-597 ◽  
Author(s):  
Takanori Sasaki ◽  
Yoshitake Terano ◽  
Tadayoshi Shibata ◽  
Hiroyoshi Kawamoto ◽  
Tsuyoshi Kuzuguchi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Quantitative Immunoassay

scholarly journals Gold colloid-nanostructured surfaces for enhanced piezoelectric immunosensing of staphylococcal enterotoxin A

2018 ◽  
Vol 255 ◽  
pp. 1604-1613 ◽  
Author(s):  
Maroua Ben Haddada ◽  
Michèle Salmain ◽  
Souhir Boujday
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Gold Colloid ◽  
Nanostructured Surfaces

Inhibition of allostimulated HLA-DQ and DP-specific T cells by staphylococcal enterotoxin A

1993 ◽  
Vol 36 (3) ◽  
pp. 142-148 ◽  
Author(s):  
Susan Masewicz ◽  
Jeffrey A. Ledbetter ◽  
Paul Martin ◽  
Eric Mickelson ◽  
John A. Hansen ◽  
...  
Keyword(s):  
T Cells ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Hla Dq
Sign in / Sign up

Export Citation Format

Share Document