Effects of an Alfalfa Protein Hydrolysate on the Gene Expression and Activity of Enzymes of the Tricarboxylic Acid (TCA) Cycle and Nitrogen Metabolism in Zea mays L.

2008 ◽  
Vol 56 (24) ◽  
pp. 11800-11808 ◽  
Author(s):  
Michela Schiavon ◽  
Andrea Ertani ◽  
Serenella Nardi
1996 ◽  
Vol 15 (7) ◽  
pp. 489-494 ◽  
Author(s):  
Philippe Vain ◽  
Kim R. Finer ◽  
Dean E. Engler ◽  
Richard C. Pratt ◽  
John J. Finer

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Hiroko Iijima ◽  
Atsuko Watanabe ◽  
Junko Takanobu ◽  
Masami Yokota Hirai ◽  
Takashi Osanai

The tricarboxylic acid (TCA) cycle and pyruvate metabolism of cyanobacteria are unique and important from the perspectives of biology and biotechnology research. Rre37, a response regulator induced by nitrogen depletion, activates gene expression related to sugar catabolism. Our previous microarray analysis has suggested that Rre37 controls the transcription of genes involved in sugar catabolism, pyruvate metabolism, and the TCA cycle. In this study, quantitative real-time PCR was used to measure the transcript levels of 12 TCA cycle genes and 13 pyruvate metabolism genes. The transcripts of 6 genes (acnB,icd,ppc,pyk1,me, andpta) increased after 4 h of nitrogen depletion in the wild-type GT strain but the induction was abolished byrre37overexpression. The repression of gene expression offumC, ddh, andackAcaused by nitrogen depletion was abolished byrre37overexpression. The expression ofmewas differently affected byrre37overexpression, compared to the other 24 genes. These results indicate that Rre37 differently controls the genes of the TCA cycle and pyruvate metabolism, implying the key reaction of the primary in this unicellular cyanobacterium.


2011 ◽  
Vol 109 (2) ◽  
pp. 271-286 ◽  
Author(s):  
Lifang Sun ◽  
Ying Wu ◽  
Shengzhong Su ◽  
Hongkui Liu ◽  
Guang Yang ◽  
...  

2019 ◽  
Vol 50 (Special) ◽  
Author(s):  
Shenawa & Alfalahi

Methylation Sensitive Amplification Polymorphism (MSAP) was used to characterize the alterations in DNA methylation in maize (Zea mays L.) inbred lines and their half-daillels affected by plant densities (213333 plant h-1 and 13333 plant h-1). The two restriction was enzymes ( HpaII and MspI) succeeded in diagnosing a total of 23 specific loci, most of (22 loci) were Methylation Sensitive Loci (MSL), while the only one NML (No Methylated Loci) was monomorphic. Thirteen out of 22 MSL loci polymorphic, recording a were polymorphism percentage of 59%. Results of FeSOD gene expression cleared the different response of maize inbreds and hybrids to high plant density stress. Generally, the expression of  the targeted gene was increased in plants submitted to high plant density stress compared with low density. The inbred 3 and its single hybrid 1×3 achieved the highest level of gene expression under high planting density (5505.7 and 21098.6 copy, respectively), meanwhile, inbred 5 and it's single hybrid 4×5 gained the maximum level of FeSOD expression at the low plant density (8317.6 and 6862.1 copy, respectively). The response reached to its maximum limit in many of those genotypes, some other genotypes showed relatively steady performance along with higher stress, such as parent 1, that gave the lowest number of gene copies in both, high and low plant density (1375.8 and 1569.5 copy, respectively).


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