Antioxidant status and their enhancements strategies for water stress tolerance in chickpea

Water stress executes severe influences on the plant growth and development through modifying physio-chemical properties. Therefore, a field experiment was designed to evaluate the antioxidant status and their enhancements strategies for water stress tolerance in chickpea on loam and clay loam soils under agro-ecological conditions of Arid Zone Research Institute, Bahawalpur (29.3871 °N, 71.653 °E) and Cholistan farm near Derawer (28.19°N, 71.80°E) of Southern Punjab, Pakistan during winter 2014-15. Experimental treatments comprised of two chickpea cultivars i.e. Bhakhar 2011 (drought tolerant) and DUSHT (drought sensitive), two water stress levels i.e. water stress at flowering stage and water stress at flowering + pod formation + grain filling stage including well watered (control) and two exogenous application of osmoprotectants i.e. glycine betaine (GB) 20 ppm and proline 10 uM including distilled water (control). Results indicated that water stress at various growth stages adversely affects the growth, yield and quality attributes of both chickpea cultivars. Exogenous application of GB and proline improved the growth, yield and quality parameters of both chickpea cultivars even under water stress conditions. However, superior results were obtained with exogenously applied GB on Bhakhar 2011 under well-watered conditions. Similarly, foliar spray of GB on chickpea cultivar Bhakhar 2011 under stress at flowering + pod formation + grain filling stage produced maximum superoxide dismutase, peroxidase and catalase contents. These results suggested that application of GB mitigates the adverse effects of water stress and enhanced tolerance in chickpea mainly due to higher antioxidant enzymes activity, demonstrating the protective measures of plant cells in stress condition. Hence, antioxidants status might be a suitable method for illustrating water stress tolerance in chickpea.

Effects of Irrigation Levels on the Yield of Chickpea (Cicer Arietinum Linn) Due to Incidence of Helicoverpa Armigera

Effects of four different irrigation levels viz., One irrigation at 50 per cent flowering stage, one irrigation at grain filling/pod initiation stage, two irrigations (one at 50 per cent flowering + one at grain filling stage), control (without irrigation) on the incidence of gram pod borer, Helicoverpa armigera (Hubner) on chickpea were studied during Rabi, 2014-15 and 2015-16 at Research Farm, College of Agriculture, Bikaner (Rajasthan), India. Results revealed that the highest mean larval population (3.12 larvae per 5 plants) and maximum pod damage (33.24%) were recorded in the crop which was irrigated twice viz., one at 50 per cent flowering stage and second at grain filling stage. The lowest larval population (2.03 larvae per 5 plants) with minimum pod damage (8.34%) was recorded in the crop where irrigation was not given. The maximum seed yield (11.05 q/ha) was obtained from the crop which was irrigated two times and minimum seed yield (7.56 q/ha) was obtained in control. Bangladesh J. Bot. 50(4): 1203-1207, 2021 (December)

NUMERICAL ANALYSIS OF INFUSION STRATEGIES IN VACUUM ASSISTED RESIN TRANSFER MOLDING (VARTM) PROCESS

The Liquid composite Molding (LCM) process, such as Vacuum Assisted Resin Transfer Molding (VARTM), offers a fast and high-quality production of composites laminates. In the VARTM process, the simulation tool is found beneficial to predict and solve composite manufacturing issues. The part quality is dependent on the resin mold filling stage in the VARTM process. The infiltration of resin into a porous fibrous medium is taken place during the resin mold filling stage. The permeability has a crucial role during the resin mold filling stage. In this study, simulation of resin infusion through multiple injection gates is discussed. The various infusion schemes are simulated to identify defect-free composite manufacturing. The simulation approach is applied to five different stacking sequences of reinforcements. In this transient simulation study, permeability and resin viscosity is essential inputs for the resin flow. The simulation approach found that a gating scheme plays a vital role in mold filling time and defect-free composite fabrication. It is found that the line gating system can be useful for fast mold filling over the point gating system.

Relationship Between the Vascular Bundle Structure of Panicle Branches and the Filling of Inferior Spikelets in Large-Panicle Japonica Rice

The vascular bundles of rice panicles serve to connect the source and the sink, as well as serving as a channel for the transportation of materials. In this study, two homozygous japonica rice strains were used as materials. The vascular bundle structures of the branches in different positions within a rice panicle were observed, and their cross-sectional areas were calculated. In addition, the ultrastructure of the central large vascular bundle (LVB) phloem in the rachillae of superior spikelets (SS) and inferior spikelets (IS) was observed during the grain filling period. Moreover, the soluble sugar and protein contents of the SS and IS rachillae were also measured to study whether the differences in the structure of vascular bundles of the branches were related to the plumpness of grain at different positions. The results showed that vascular bundle cross-sectional areas of the basal primary branches were greater than those in the upper primary branches. Moreover, there was little difference in the areas of vascular bundles between the basal secondary branches and upper secondary branches. However, the vascular bundle areas of the IS rachillae were lower than those in the SS rachillae. Therefore, we believe that the poor vascular tissue channel of the IS rachillae could be the limiting factor in IS plumpness. The results also showed that a similar time course in the degradation pattern of some organelles of the sieve elements and companion cells in central LVB was observed in the SS rachillae and IS rachillae during the grain filling period. Compared with the IS rachillae, more abundant mitochondria and plasmodesmata were found in the companion cells of SS rachillae at the beginning of the filling stage, while no significant differences between SS and IS rachillae were identified at the middle and late filling stages, which implies that the SS rachillae were relatively more effective at transportation compared with the IS rachillae at the initial filling stage. Therefore, the undeveloped vascular bundles of the IS rachillae and their poor physiology and lack of ability to transport at the initial filling stages could be the limiting factor in IS plumpness.

The influence of rhizosphere soil fungal diversity and complex community structure on wheat root rot disease

Wheat root rot disease due to soil-borne fungal pathogens leads to tremendous yield losses worth billions of dollars worldwide every year. It is very important to study the relationship between rhizosphere soil fungal diversity and wheat roots to understand the occurrence and development of wheat root rot disease. A significant difference in fungal diversity was observed in the rhizosphere soil of healthy and diseased wheat roots in the heading stage, but the trend was the opposite in the filling stage. The abundance of most genera with high richness decreased significantly from the heading to the filling stage in the diseased groups; the richness of approximately one-third of all genera remained unchanged, and only a few low-richness genera, such as Fusarium and Ceratobasidium, had a very significant increase from the heading to the filling stage. In the healthy groups, the abundance of most genera increased significantly from the heading to filling stage; the abundance of some genera did not change markedly, or the abundance of very few genera increased significantly. Physical and chemical soil indicators showed that low soil pH and density, increases in ammonium nitrogen, nitrate nitrogen and total nitrogen contributed to the occurrence of wheat root rot disease. Our results revealed that in the early stages of disease, highly diverse rhizosphere soil fungi and a complex community structure can easily cause wheat root rot disease. The existence of pathogenic fungi is a necessary condition for wheat root rot disease, but the richness of pathogenic fungi is not necessarily important. The increases in ammonium nitrogen, nitrate nitrogen and total nitrogen contributed to the occurrence of wheat root rot disease. Low soil pH and soil density are beneficial to the occurrence of wheat root rot disease.

Comparison of photosynthetic activity and heat tolerance between near isogenic lines of wheat with different photosynthetic rates

Wheat (Triticum aestivum L.) is one of the most important crops in the world, but the yield and quality of wheat are highly susceptible to heat stress, especially during the grain-filling stage. Therefore, it is crucial to select high-yield and high-temperature-resistant varieties for food cultivation. There is a positive correlation between the yield and photosynthetic rate of wheat during the entire grain-filling stage, but few studies have shown that lines with high photosynthetic rates can maintain higher thermotolerance at the same time. In this study, two pairs of wheat near isogenic lines (NILs) with different photosynthetic rates were used for all experiments. Our results indicated that under heat stress, lines with a high photosynthetic rate could maintain the activities of photosystem II (PSII) and key Calvin cycle enzymes in addition to their higher photosynthetic rates. The protein levels of D1 and HSP70 were significantly increased in the highly photosynthetic lines, which contributed to maintaining high photosynthetic rates and ensuring the stability of the Calvin cycle under heat stress. Furthermore, we found that lines with a high photosynthetic rate could maintain high antioxidant enzyme activity to scavenge reactive oxygen species (ROS) and reduce ROS accumulation better than lines with a low photosynthetic rate under high-temperature stress. These findings suggest that lines with high photosynthetic rates can maintain a higher photosynthetic rate despite heat stress and are more thermotolerant than lines with low photosynthetic rates.

Dissecting of the Deterioration in Eating Quality for Erect Panicle (Ep) Type High Yield Japonica Super Rice in Northest China

Ep type is an important morphological improvement (following dwarf breeding and ideal plant type) to adapt to super high yield breeding of rice, which shows a pleiotropic effect in increasing grain yield and nitrogen use efficiency (NUE) in rice. Nevertheless, it remains unclear whether Ep has adverse effects on eating quality and its regulatory of increasing nitrogen uptake and assimilation. In this study, we developed a pair of near-isogenic lines (NILs) of dep1 (NIL-Ep, NIL-Non Ep) in the Liaogeng 5 (LG5) and Akihikari (AKI) backgrounds. Here, we report that rice plants NIL-Ep have more grain numbers per panicle in middle to bottom spike positions than plants NIL-non Ep. This part of increased grain not only is the key factor to increase the yield, but also is the reason to reduce the eating quality. The content of prolamin and glutelin in the grain increased significantly, which resulted in higher hardness and worse viscosity of rice after cooking. Additionally, the activity of several essential enzymes catalyzing nitrogen metabolism is higher in the NIL-Ep line than in NIL-non Ep line, especially from the mid to late grain filling stage. Based on these results, we conclude that Ep positively regulates grain protein accumulation primarily through enhance the activity of enzyme enroll nitrogen assimilation and redistribution during the mid to late grain-filling stage, resulting in excessive accumulation of grain protein and decreased the quality of eating.