ZSM-5 zeolites were hydrothermally synthesized in three different seeding pathways under the direction of tetrapropylammonium bromide (TPABr) template. In order to investigate the seeding effect in ZSM-5 crystallization process, ZSM-5 crystals and pre-fabricated MFI-type nanoseeds were added into the original self-induction system, respectively. The final ZSM-5 zeolites were systematically investigated based on XRD (X-ray diffraction), SEM (scanning electron micrograph), TEM (transmission electron micrograph), nitrogen adsorption characterizations and NH3 -TPD (ammonia-temperature programmed desorption). The self-induction system produced ca. 20 μm ZSM-5 zeolite displaying hexagonally uniform prisms. After the addition of ZSM-5 crystal seeds, the crystal sizes were decreased greatly to ca. 5 μm. When MFI-type nanoseeds were adopted, irregular aggregate particles consisting of 20–50 nm primary particles were rapidly synthesized. The varied hydrothermal crystallization kinetics of the three synthesis system was also explored. Adjusting the seed agents alone, ZSM-5 crystals with diverse structural, morphological, textural and hydrothermal behaviors could be fabricated conveniently. The three ZSM-5 zeolites loaded by 0.05 wt.% Pt were assessed for the xylene isomerization reaction to investigate the particle size effect on the catalytic properties.