Lateral Organization of a Membrane Protein in a Supported Binary Lipid Domain:  Direct Observation of the Organization of Bacterial Light-Harvesting Complex 2 by Total Internal Reflection Fluorescence Microscopy

Langmuir ◽  
2006 ◽  
Vol 22 (12) ◽  
pp. 5412-5418 ◽  
Author(s):  
Takehisa Dewa ◽  
Ryuta Sugiura ◽  
Yoshiharu Suemori ◽  
Miku Sugimoto ◽  
Toshikazu Takeuchi ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Fluorescence Microscopy ◽  
Direct Observation ◽  
Total Internal Reflection ◽  
Light Harvesting ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Light Harvesting Complex ◽  
Lipid Domain ◽  
Lateral Organization

scholarly journals Imaging Constitutive Exocytosis with Total Internal Reflection Fluorescence Microscopy

2000 ◽  
Vol 149 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Jan Schmoranzer ◽  
Mark Goulian ◽  
Dan Axelrod ◽  
Sanford M. Simon
Keyword(s):  
Plasma Membrane ◽  
Membrane Protein ◽  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Fluorescent Protein ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Green Fluorescent ◽  
Kinetics Of ◽  
Flattening Process

Total internal reflection fluorescence microscopy has been applied to image the final stage of constitutive exocytosis, which is the fusion of single post-Golgi carriers with the plasma membrane. The use of a membrane protein tagged with green fluorescent protein allowed the kinetics of fusion to be followed with a time resolution of 30 frames/s. Quantitative analysis allowed carriers undergoing fusion to be easily distinguished from carriers moving perpendicularly to the plasma membrane. The flattening of the carriers into the plasma membrane is seen as a simultaneous rise in the total, peak, and width of the fluorescence intensity. The duration of this flattening process depends on the size of the carriers, distinguishing small spherical from large tubular carriers. The spread of the membrane protein into the plasma membrane upon fusion is diffusive. Mapping many fusion sites of a single cell reveals that there are no preferred sites for constitutive exocytosis in this system.

scholarly journals Fast high-resolution 3D total internal reflection fluorescence microscopy by incidence angle scanning and azimuthal averaging

2014 ◽  
Vol 111 (48) ◽  
pp. 17164-17169 ◽  
Author(s):  
Jérôme Boulanger ◽  
Charles Gueudry ◽  
Daniel Münch ◽  
Bertrand Cinquin ◽  
Perrine Paul-Gilloteaux ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Incidence Angle ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence
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