Intracellular Delivery of the Reactive Oxygen Species Generating Agent d-Penicillamine upon Conjugation to Poly-l-glutamic Acid

2010 ◽  
Vol 7 (3) ◽  
pp. 854-862 ◽  
Author(s):  
Saurabh Wadhwa ◽  
Russell J. Mumper
Keyword(s):  
Reactive Oxygen Species ◽  
Glutamic Acid ◽  
Reactive Oxygen ◽  
Intracellular Delivery ◽  
Oxygen Species

Enhanced poly(γ-glutamic acid) production by H2O2-induced reactive oxygen species in the fermentation ofBacillus subtilisNX-2

2015 ◽  
Vol 63 (5) ◽  
pp. 625-632 ◽  
Author(s):  
Bao Tang ◽  
Dan Zhang ◽  
Sha Li ◽  
Zongqi Xu ◽  
Xiaohai Feng ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Glutamic Acid ◽  
Acid Production ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Glutamic Acid Production

scholarly journals Islet glutamic acid decarboxylase modified by reactive oxygen species is recognized by antibodies from patients with type 1 diabetes mellitus

2001 ◽  
Vol 126 (2) ◽  
pp. 242-249 ◽  
Author(s):  
S. M. Trigwell ◽  
P. M. Radford ◽  
S. R. Page ◽  
A. C. Loweth ◽  
R. F. L. James ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Reactive Oxygen Species ◽  
Type 1 Diabetes ◽  
Glutamic Acid ◽  
Type 1 Diabetes Mellitus ◽  
Glutamic Acid Decarboxylase ◽  
Reactive Oxygen ◽  
Acid Decarboxylase ◽  
Oxygen Species

Reactive Oxygen Species-Responsive Protein Modification and Its Intracellular Delivery for Targeted Cancer Therapy

2014 ◽  
Vol 53 (49) ◽  
pp. 13444-13448 ◽  
Author(s):  
Ming Wang ◽  
Shuo Sun ◽  
Caleb I. Neufeld ◽  
Bernardo Perez-Ramirez ◽  
Qiaobing Xu
Keyword(s):  
Reactive Oxygen Species ◽  
Cancer Therapy ◽  
Protein Modification ◽  
Reactive Oxygen ◽  
Intracellular Delivery ◽  
Oxygen Species ◽  
Targeted Cancer Therapy

Reactive Oxygen Species-Responsive Protein Modification and Its Intracellular Delivery for Targeted Cancer Therapy

2014 ◽  
Vol 126 (49) ◽  
pp. 13662-13666 ◽  
Author(s):  
Ming Wang ◽  
Shuo Sun ◽  
Caleb I. Neufeld ◽  
Bernardo Perez-Ramirez ◽  
Qiaobing Xu
Keyword(s):  
Reactive Oxygen Species ◽  
Cancer Therapy ◽  
Protein Modification ◽  
Reactive Oxygen ◽  
Intracellular Delivery ◽  
Oxygen Species ◽  
Targeted Cancer Therapy

scholarly journals Enhanced Synthesis of Poly Gamma Glutamic Acid by Increasing the Intracellular Reactive Oxygen Species in the Bacillus licheniformis Δ1-pyrroline-5-carboxylate Dehydrogenase Gene ycgN Deficient Strain

2018 ◽  
Author(s):  
Bichan Li ◽  
Dongbo Cai ◽  
Shiying Hu ◽  
Anting Zhu ◽  
Zhili He ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Glutamic Acid ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Proline Metabolism ◽  
Cellular Processes ◽  
Proline Catabolism ◽  
Double Mutant Strain ◽  
Wide Range

AbstractPoly gamma glutamic acid (γ-PGA) is an anionic polyamide with numerous applications. Proline metabolism influences the formation of reactive oxygen species (ROS), and is involved in a wide range of cellular processes. However, the relation between proline metabolism and γ-PGA synthesis has not yet been analyzed. In this study, our results indicated that the deletion of Δ1-pyrroline-5-carboxylate dehydrogenase encoded gene ycgN resulted in 85.22% higher yield of γ-PGA in B. licheniformis WX-02. But the deletion of proline dehydrogenase encoded gene ycgM had no effect on γ-PGA synthesis. Meanwhile, a 2.92-fold higher level of P5C was detected in ycgN deficient strain WXΔycgN, while the P5C levels in WXΔycgM and double mutant strain WXΔycgMN remained the same, compared to WX-02. The ROS level of WXΔycgN was 1.18-fold higher than that of WX-02, and the addition of n-acetylcysteine (antioxidant) into medium could decrease its ROS level, further reduced the γ-PGA yield. Our results showed that proline catabolism played an important role in maintaining ROS homeostasis, and the deletion of ycgN caused P5C accumulation, which induced a transient ROS signal to promote γ-PGA synthesis in B. licheniformis.Importanceγ-PGA is an anionic polyamide with various applications in biomedical and industrial fields. Proline metabolism influences the intracellular reactive oxygen species (ROS) and is involved in a wide range of cellular processes. Here, we report the effects of proline metabolism on γ-PGA synthesis. Our results indicated that deletion of ycgN promoted the synthesis of γ-PGA by increasing the intracellular levels of Δ1-pyrroline-5-carboxylate to generate a transient ROS signal in B. licheniformis WX-02. This study provides the valuable information that enhanced synthesis of γ-PGA by knocking out of ycgN.

Reactive oxygen species (ROS) responsive PEG–PCL nanoparticles with pH-controlled negative-to-positive charge reversal for intracellular delivery of doxorubicin

2015 ◽  
Vol 3 (48) ◽  
pp. 9397-9408 ◽  
Author(s):  
Hongzhang Deng ◽  
Xuefei Zhao ◽  
Jinjian Liu ◽  
Liandong Deng ◽  
Jianhua Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Positive Charge ◽  
Reactive Oxygen ◽  
Intracellular Delivery ◽  
Oxygen Species ◽  
Triggered Release ◽  
Charge Reversal ◽  
Active Loading ◽  
Ph Controlled

The charge-conversional behavior and ROS-sensitivity of mPEG-ros-P(CL-co-DCL) NPs for active loading and triggered release of DOX.

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