Liposome Formation from Bile Salt–Lipid Micelles in the Digestion and Drug Delivery Model FaSSIFmod Estimated by Combined Time-Resolved Neutron and Dynamic Light Scattering

Abstract Dynamic Light Scattering (DLS) is a ubiquitous and non-invasive measurement for the characterization of nano- and micro-scale particles in dispersion. The sixth power relationship between scattered intensity and particle radius is simultaneously a primary advantage whilst rendering the technique sensitive to unwanted size fractions from unclean lab-ware, dust and aggregated & dynamically aggregating sample, for example. This can make sample preparation iterative, challenging and time consuming and often requires the use of data filtering methods that leave an inaccurate estimate of the steady state size fraction and may provide no knowledge to the user of the presence of the transient fractions. A revolutionary new approach to DLS measurement and data analysis is presented whereby the statistical variance of a series of individually analysed, extremely short sub-measurements is used to classify data as steady-state or transient. Crucially, all sub-measurements are reported, and no data are rejected, providing a precise and accurate measurement of both the steady state and transient size fractions. We demonstrate that this approach deals intrinsically and seamlessly with the transition from a stable dispersion to the partially- and fully-aggregated cases and results in an attendant improvement in DLS precision due to the shorter sub measurement length and the classification process used.

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Microemulsions for Dermal Drug Delivery Studied by Dynamic Light Scattering: Effect of Interparticle Interactions in Oil‐in‐Water Microemulsions

Journal of Pharmaceutical Sciences ◽

10.1002/jps.10333 ◽

2003 ◽

Vol 92 (4) ◽

pp. 730-738 ◽

Cited By ~ 26

Author(s):

Anuj Shukla ◽

Martin Janich ◽

Konstanze Jahn ◽

Reinhard H.H. Neubert

Keyword(s):

Drug Delivery ◽

Light Scattering ◽

Dynamic Light Scattering ◽

Interparticle Interactions ◽

Scattering Effect ◽

Oil In Water ◽

Dermal Drug Delivery ◽

Light Scattering Effect

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Polyethyleneimine Modified Biocompatible Poly(N-isopropylacrylamide)-Based Nanogels for Drug Delivery

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2008.236 ◽

2008 ◽

Vol 8 (5) ◽

pp. 2377-2384 ◽

Cited By ~ 17

Author(s):

Chang-Yun Quan ◽

Hua Wei ◽

Yun-Xia Sun ◽

Si-Xue Cheng ◽

Kun Shen ◽

...

Keyword(s):

Electron Microscopy ◽

Drug Delivery ◽

Transmission Electron Microscopy ◽

Light Scattering ◽

Dynamic Light Scattering ◽

Transmission Electron ◽

Potential Applications ◽

Different Temperatures ◽

The Mean ◽

Stimuli Sensitive

A series of biocompatible and stimuli-sensitive poly(N-isopropylacrylamide-co-propyl acrylic acid) (P(NIPAAm-co-PAAc)) nanogels were synthesized by emulsion polymerization. In addition, polyethyleneimine (PEI) was further grafted to modify the PNIPAAm-based nanogels. The P(NIPAAm-co-PAAc)-g-PEI nanogels exhibited good thermosensitivity as well as pH sensitivity. Transmission electron microscopy (TEM) showed that the P(NIPAAm-co-PAAc)-g-PEI and P(NIPAAm-co-PAAc) nanogels displayed well dispersed spherical morphology. The mean sizes of the nanogels measured by dynamic light scattering (DLS) were from 100 nm to 500 nm at different temperatures. The cytotoxicity study indicated P(NIPAAm-co-PAAc) nanogels exhibited a better biocompatibility than both PNIPAAm nanogel and P(NIPAAm-co-PAAc)-g-PEI nanogel although all the three kinds of nanogels did not exhibit apparent cytotoxicity. The drug-loaded nanogels, especially the PEI-grafted nanogels, showed temperature-trigged controlled release behaviors, indicating the potential applications as an intelligent drug delivery system.

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