Direct Tandem Mass Spectrometry Reveals Limitations in Protein Profiling Experiments for Plasma Biomarker Discovery

2005 ◽  
Vol 4 (3) ◽  
pp. 972-981 ◽  
Author(s):  
John M. Koomen ◽  
Donghui Li ◽  
Lian-chun Xiao ◽  
Thomas C. Liu ◽  
Kevin R. Coombes ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Biomarker Discovery ◽  
Protein Profiling ◽  
Tandem Mass ◽  
Plasma Biomarker

scholarly journals A Dataset of Human Liver Proteins Identified by Protein Profiling Via Isotope-coded Affinity Tag (ICAT) and Tandem Mass Spectrometry: Fig. 1.

2004 ◽  
Vol 3 (10) ◽  
pp. 1039-1041 ◽  
Author(s):  
Wei Yan ◽  
Hookeun Lee ◽  
Eric W. Deutsch ◽  
Catherine A. Lazaro ◽  
Weiliang Tang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Human Liver ◽  
Protein Profiling ◽  
Tandem Mass ◽  
Affinity Tag

scholarly journals Protein profiling of sickle cell versus control RBC core membrane skeletons by ICAT technology and tandem mass spectrometry

2006 ◽  
Vol 11 (3) ◽  
Author(s):  
Jose Chou ◽  
Pankaj Choudhary ◽  
Steven Goodman
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Sickle Cell ◽  
Membrane Skeleton ◽  
Protein Profiling ◽  
Tandem Mass ◽  
Proteomic Approach ◽  
Significant Difference ◽  
The Mean ◽  
Cell Versus

AbstractA proteomic approach using a cleavable ICAT reagent and nano-LC ESI tandem mass spectrometry was used to perform protein profiling of core RBC membrane skeleton proteins between sickle cell patients (SS) and controls (AA), and determine the efficacy of this technology. The data was validated through Peptide/Protein Prophet and protein ratios were calculated through ASAPratio. Through an ANOVA test, it was determined that there is no significant difference in the mean ratios from control populations (AA1/AA2) and sickle cell versus control populations (AA/SS). The mean ratios were not significantly different from 1.0 in either comparison for the core skeleton proteins (α spectrin, β spectrin, band 4.1 and actin). On the natural-log scale, the variation (standard deviation) of the method was determined to be 14.1% and the variation contributed by the samples was 13.8% which together give a total variation of 19.7% in the ratios.

scholarly journals The Application of New Software Tools to Quantitative Protein Profiling Via Isotope-coded Affinity Tag (ICAT) and Tandem Mass Spectrometry

2003 ◽  
Vol 2 (7) ◽  
pp. 426-427 ◽  
Author(s):  
Priska D. von Haller ◽  
Eugene Yi ◽  
Samuel Donohoe ◽  
Kelly Vaughn ◽  
Andrew Keller ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Software Tools ◽  
Protein Profiling ◽  
Tandem Mass ◽  
Affinity Tag
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