scholarly journals Protein profiling of sickle cell versus control RBC core membrane skeletons by ICAT technology and tandem mass spectrometry

2006 ◽  
Vol 11 (3) ◽  
Author(s):  
Jose Chou ◽  
Pankaj Choudhary ◽  
Steven Goodman
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Sickle Cell ◽  
Membrane Skeleton ◽  
Protein Profiling ◽  
Tandem Mass ◽  
Proteomic Approach ◽  
Significant Difference ◽  
The Mean ◽  
Cell Versus

AbstractA proteomic approach using a cleavable ICAT reagent and nano-LC ESI tandem mass spectrometry was used to perform protein profiling of core RBC membrane skeleton proteins between sickle cell patients (SS) and controls (AA), and determine the efficacy of this technology. The data was validated through Peptide/Protein Prophet and protein ratios were calculated through ASAPratio. Through an ANOVA test, it was determined that there is no significant difference in the mean ratios from control populations (AA1/AA2) and sickle cell versus control populations (AA/SS). The mean ratios were not significantly different from 1.0 in either comparison for the core skeleton proteins (α spectrin, β spectrin, band 4.1 and actin). On the natural-log scale, the variation (standard deviation) of the method was determined to be 14.1% and the variation contributed by the samples was 13.8% which together give a total variation of 19.7% in the ratios.

Rapid diagnosis of maple syrup urine disease in blood spots from newborns by tandem mass spectrometry

1995 ◽  
Vol 41 (1) ◽  
pp. 62-68 ◽  
Author(s):  
D H Chace ◽  
S L Hillman ◽  
D S Millington ◽  
S G Kahler ◽  
C R Roe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Maple Syrup Urine Disease ◽  
Dried Blood Spots ◽  
Tandem Mass ◽  
Inhibition Assay ◽  
Maple Syrup ◽  
Blood Spots ◽  
Urine Disease ◽  
The Mean

Abstract We report a new method for the diagnosis of maple syrup urine disease (MSUD) from dried blood spots on newborn screening cards based on tandem mass spectrometry (MS-MS). The mean +/- SD concentration of Leu plus Ile in normal newborns was 151 +/- 47 mumol/L (n = 1096); for Val, 131 +/- 58 mumol/L (n = 791). SDs were lower when the concentrations of these amino acids were expressed relative to that of Phe. The mean ratio for Leu + Ile to Phe was 2.5 +/- 0.49; for Val to Phe, 2.18 +/- 0.51. These results compare well with values previously reported in the literature. With these criteria, samples from a collection categorized by a bacterial inhibition assay as normal or falsely positive for MSUD were normal by MS-MS [(Leu + Ile): Phe < 5.0]. Samples from confirmed MSUD patients were categorized as abnormal [(Leu+Ile): Phe > 9.0] by MS-MS.

A Significant Difference in the Blood Carnitine Values Obtained by the Enzymatic Cycling and Tandem Mass Spectrometry Methods

2018 ◽  
Vol 64 (01+02/2018) ◽  
Author(s):  
Jun Kido ◽  
Hironobu Inoue ◽  
Yosuke Suzuki ◽  
Motoko Tanaka ◽  
Hiroshi Mitsubuchi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Enzymatic Cycling ◽  
Significant Difference

scholarly journals Newborn screening for sickle cell disorders using tandem mass spectrometry: three years’ experience of using a protocol to detect only the disease states

2017 ◽  
Vol 54 (5) ◽  
pp. 601-611 ◽  
Author(s):  
Stuart J Moat ◽  
Derek Rees ◽  
Roanna S George ◽  
Lawrence King ◽  
Alan Dodd ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Newborn Screening ◽  
Sickle Cell ◽  
Tandem Mass ◽  
Wild Type ◽  
Disease States ◽  
Age Related ◽  
Screening Protocol ◽  
Sickle Cell Disorders

Background Tandem mass spectrometry (MS/MS) has recently become an alternative method for the newborn screening of sickle cell disorders (SCD), as it is able to detect haemoglobin (Hb) peptides following digestion of bloodspots with trypsin. Using the SpOtOn Diagnostics Reagent Kit, we previously developed a screening protocol to detect only the disease states of SCD, using action values based on the ratio between the variant Hb peptide to wild-type peptide abundances for the HbS, C, DPunjab, OArab, E and Lepore peptides. Methods Action values using the ratios between the wild type HbA (ßT1-3) peptides and the foetal Hb (γT2) peptide were developed to identify bloodspot samples from premature and transfused infants. An evaluation was undertaken to assess the transferability of the action values onto an additional MS/MS instrument. We report here our experience using this MS/MS protocol. Results During a three-year period, we screened 100,456 babies and identified 10 SCD cases (1 HbS/HPFH, 5 HbS/S and 4 HbS/C) and a case of HbE/ß-thalassaemia that was identified as a by-product. The Hb variant to wild-type peptide ratio action values were transferable to a second MS/MS instrument. Our protocol prevented the identification of an estimated 810 carrier infants. Gestational age-related action values for HbA to HbF peptide ratios were required to minimize the number of samples referred for second-line testing to exclude ß-thalassaemia. Conclusion MS/MS is a robust alternative screening technology for SCD; in addition, it also optimizes the use of equipment and expertise that currently exist in newborn screening laboratories.

scholarly journals A Dataset of Human Liver Proteins Identified by Protein Profiling Via Isotope-coded Affinity Tag (ICAT) and Tandem Mass Spectrometry: Fig. 1.

2004 ◽  
Vol 3 (10) ◽  
pp. 1039-1041 ◽  
Author(s):  
Wei Yan ◽  
Hookeun Lee ◽  
Eric W. Deutsch ◽  
Catherine A. Lazaro ◽  
Weiliang Tang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Human Liver ◽  
Protein Profiling ◽  
Tandem Mass ◽  
Affinity Tag

scholarly journals Analysis of Acrylamide in Dried Blood Spots of Lung Cancer Patients by Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Yahdiana Harahap ◽  
Camilla Elysia ◽  
Zenshiny Starlin ◽  
Achmad Mulawarman Jayusman
Keyword(s):  
Mass Spectrometry ◽  
Lung Cancer ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Cancer Patients ◽  
Tandem Mass ◽  
Lung Cancer Patients ◽  
Chromatography Tandem Mass Spectrometry ◽  
Significant Difference ◽  
Liquid Chromatography Tandem Mass

Acrylamide (AA) is a carcinogenic substance found in food, cigarette smoke and in an environment exposed to acrylamide. This study aims to analyze AA levels in dried blood spot (DBS) samples of lung cancer patients with smoking record, without smoking record, and also in the negative blank. Analysis of AA levels was determined by liquid chromatography tandem mass spectrometry (LC-MS/MS) and DBS extraction using protein precipitation techniques. Mass detection was done using positive electron spray ionization (ESI) and multiple reaction monitoring (MRM) type with m/z values of 71.99 > 55.23 for acrylamide and m/z 260.16 > 116.04 for propranolol as the internal standard. AA levels in lung cancer patients with smoking record is in the range of 4.670 μg/mL to 11.986 μg/mL. AA levels in lung cancer patients without smoking record is in the range of 2.041 μg/mL to 12.702 μg/mL. Data on AA levels on negative blanks is in the range of 2.72 μg/mL to 3.51 μg/mL. The results of the independent sample t-test (p>0.05) showed that AA levels in patients with smoking record and those without smoking record did not differ significantly. Then, the Mann-Whitney test was performed between the lung cancer group and the negative blank group and a significant difference was found between the two groups (p<0.05).

Investigation of alanine, propionylcarnitine (C3) and 3-hydroxyisovalerylcarnitine (C5-OH) levels in patients with partial biotinidase deficiency

2019 ◽  
Vol 44 (4) ◽  
pp. 482-486
Author(s):  
Halil Kazanasmaz ◽  
Meryem Karaca
Keyword(s):  
Mass Spectrometry ◽  
Metabolic Disease ◽  
Screening Program ◽  
Biotinidase Deficiency ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Significant Difference ◽  
Liquid Chromatography Tandem Mass ◽  
The Mean ◽  
Biotinidase Activity

Abstract Background Biotinidase deficiency is a treatable metabolic disease that can be seen with various neurological and dermatological complications. Biomarkers such as alanine, propionylcarnitine (C3) and 3-hydroxyisovalerylcarnitine (C5-OH), which are used to diagnose biotinidase deficiency, are also present. Materials and methods In cases with partial biotinidase deficiency and normal biotinidase activity, alanine, C3 and C5-OH levels were compared in the field by liquid chromatography-tandem mass spectrometry. Results There was no significant difference between subjects with partial biotinidase deficiency and those with normal biotinidase activity between C3 and C5-OH levels. The mean alanine levels in heel blood and plasma were significantly higher than those with normal biotinidase activity in patients with partial biotinidase deficiency. Conclusion In cases with partial biotinidase deficiency, the heel blood alanine level that can be detected in the neonatal screening program may be a leading marker in diagnosis.

scholarly journals Testosterone Metabolic Clearance and Production Rates Determined by Stable Isotope Dilution/Tandem Mass Spectrometry in Normal Men: Influence of Ethnicity and Age

2004 ◽  
Vol 89 (6) ◽  
pp. 2936-2941 ◽  
Author(s):  
Christina Wang ◽  
Don H. Catlin ◽  
Borislav Starcevic ◽  
Andrew Leung ◽  
Emma DiStefano ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Tandem Mass Spectrometry ◽  
White Men ◽  
Constant Infusion ◽  
Metabolic Clearance ◽  
Tandem Mass ◽  
Stable Isotope Dilution ◽  
The Mean ◽  
Normal Men

Abstract The metabolic clearance rate (MCRT) and production rate (PRT) of testosterone (T) were measured using constant infusion of trideuterated (d3) T and quantitating serum d3T by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Serum unlabeled T (d0T) was measured by LC-MS-MS, and serum total T (d3T + d0T) was measured by RIA. Mean MCRT (measured by LC-MS-MS) in young white men (1272 ± 168 liters/d) was not significantly different from young Asian men (1070 ± 166 liters/d). Mean PRT was also not significantly different between the two ethnic groups (whites, 9.11 ± 1.11 mg/d; Asians, 7.22 ± 1.15 mg/d; P = 0.19 using d0T data). Both the mean MCRT (812 ± 64 liters/d; P &lt; 0.01) and the PRT (3.88 ± 0.27 mg/d; P &lt; 0.001) were significantly lower in middle-aged white men when compared with their younger counterparts. The mean MCRT and PRT calculated using serum total T or d0T data showed a diurnal variation, with levels at midday significantly higher than those measured in the evening in the young (MCRT, P &lt; 0.01; PRT, P &lt; 0.001) and to a lesser extent in the older men (MCRT, P &lt; 0.05; PRT, P &lt; 0.05 using total T and P &lt; 0.001 using d0T data). We conclude that using LC-MS-MS to detect d3T in serum after constant infusion of stable isotope-labeled T allows the measurements of MCRT and PRT, which can be used to study androgen metabolism repeatedly after physiological or pharmacological interventions.

Newborn screening by tandem mass spectrometry confirms the high prevalence of sickle cell disease among German newborns

2018 ◽  
Vol 98 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Stephan Lobitz ◽  
Jeannette Klein ◽  
Annemarie Brose ◽  
Oliver Blankenstein ◽  
Claudia Frömmel
Keyword(s):  
Mass Spectrometry ◽  
Sickle Cell Disease ◽  
Tandem Mass Spectrometry ◽  
Newborn Screening ◽  
Sickle Cell ◽  
Tandem Mass ◽  
Cell Disease ◽  
High Prevalence
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