Fractionation of Complex Protein Mixture by Virtual Three-Dimensional Liquid Chromatography Based on Combined pH and Salt Steps

2008 ◽  
Vol 7 (10) ◽  
pp. 4525-4537 ◽  
Author(s):  
Zhi-Bin Ning ◽  
Qing-Run Li ◽  
Jie Dai ◽  
Rong-Xia Li ◽  
Chia-Hui Shieh ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Three Dimensional ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein

The role of proteomics in studies of protein moonlighting

2014 ◽  
Vol 42 (6) ◽  
pp. 1698-1703 ◽  
Author(s):  
Robert J. Beynon ◽  
Dean Hammond ◽  
Victoria Harman ◽  
Yvonne Woolerton
Keyword(s):  
Post Translational Modifications ◽  
Multiple Functions ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein ◽  
Quantitative Approaches

The increasing acceptance that proteins may exert multiple functions in the cell brings with it new analytical challenges that will have an impact on the field of proteomics. Many proteomics workflows begin by destroying information about the interactions between different proteins, and the reduction of a complex protein mixture to constituent peptides also scrambles information about the combinatorial potential of post-translational modifications. To bring the focus of proteomics on to the domain of protein moonlighting will require novel analytical and quantitative approaches.

Unravelling Nonspecific Adsorption of Complex Protein Mixture on Surfaces with SPR and MS

2014 ◽  
Vol 86 (19) ◽  
pp. 9612-9619 ◽  
Author(s):  
Julien Breault-Turcot ◽  
Pierre Chaurand ◽  
Jean-Francois Masson
Keyword(s):  
Nonspecific Adsorption ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein

Matrigel: A complex protein mixture required for optimal growth of cell culture

PROTEOMICS ◽  
2010 ◽  
Vol 10 (9) ◽  
pp. 1886-1890 ◽  
Author(s):  
Chris S. Hughes ◽  
Lynne M. Postovit ◽  
Gilles A. Lajoie
Keyword(s):  
Cell Culture ◽  
Optimal Growth ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein

Comparison of displacement versus gradient mode for separation of a complex protein mixture by anion-exchange chromatography

2012 ◽  
Vol 901 ◽  
pp. 34-40 ◽  
Author(s):  
Robert Ahrends ◽  
Björn Lichtner ◽  
Friedrich Buck ◽  
Diana Hildebrand ◽  
Marta Kotasinska ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein ◽  
Gradient Mode

Stopped Lower Buffer Flow Electrofractionation: Simple Electrofractionation for Complex Protein Mixture

1994 ◽  
Vol 223 (1) ◽  
pp. 59-61 ◽  
Author(s):  
J.C. Pyun ◽  
H.S. Choi ◽  
J.S. Park
Keyword(s):  
Protein Mixture ◽  
Complex Protein Mixture ◽  
Complex Protein

scholarly journals Towards spatial comprehensive three-dimensional liquid chromatography: a tutorial review

2020 ◽  
Author(s):  
Thomas Themelis ◽  
Ali Amini ◽  
Jelle De Vos ◽  
Sebastiaan Eeltink
Keyword(s):  
Liquid Chromatography ◽  
Three Dimensional

Three-Dimensional Imaging of High Performance Liquid Chromatography Processes Using a Mini Positron Emission Tomograph

2017 ◽  
Vol 2 (30) ◽  
pp. 9797-9802
Author(s):  
Eva Sarkadi-Priboczki ◽  
Ivan Valastyan ◽  
Karoly Brezovcsik ◽  
David Nagy ◽  
Gabor Opposits ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Three Dimensional ◽  
Positron Emission Tomograph ◽  
Three Dimensional Imaging ◽  
Positron Emission

Aminoquinolines as fluorescent labels for hydrophilic interaction liquid chromatography of oligosaccharides

2012 ◽  
Vol 393 (8) ◽  
pp. 757-765 ◽  
Author(s):  
Weston B. Struwe ◽  
Pauline M. Rudd
Keyword(s):  
Liquid Chromatography ◽  
Hplc Analysis ◽  
Three Dimensional ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Peak Capacity ◽  
Fluorescent Labels ◽  
Sample Analysis ◽  
Hydrophilic Interaction ◽  
Optimal Excitation ◽  
Complex Sample

Abstract In this study, we investigated the potential of four different aminoquinoline (AQ) compounds as fluorescent labels for glycan analysis using hydrophilic interaction liquid chromatography (HILIC) and fluorescence detection (FLD). We confirmed the optimal excitation and emission wavelengths of 3-AQ and 6-AQ conjugated to glycan standards using three-dimensional fluorescent spectral scanning. The optimal excitation and emission wavelengths for 6-AQ were confirmed at λex=355 nm and λem=440 nm. We concluded that the optimal wavelengths for 3-AQ were λex=355 nm and λem=420 nm, which differed considerably from the wavelengths applied in previous reports. HILIC-FLD chromatograms using experimentally determined wavelengths were similar to 2-aminobenzamide controls, but the peak capacity and resolution differed significantly when published 3-AQ λex/em values were applied. Furthermore, we found that 5-AQ and 8-AQ labeled maltohexaose did not display any fluorescent pro\xadperties when used as a carbohydrate tag for HPLC analysis. Finally, we applied experimentally determined wavelengths to 3-AQ labeled N-glycans released from human IgG to illustrate changes in retention time as well as to demonstrate that AQ labeling is applicable to complex sample analysis via exoglycosidase sequencing.

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