Determination of D-amino acids using a D-amino acid oxidase biosensor with spectrophotometric and potentiometric detection

1998 ◽  
Vol 12 (2) ◽  
pp. 149-153 ◽  
Author(s):  
Silvia Sacchi ◽  
Loredano Pollegioni ◽  
Mirella S. Pilone ◽  
Carlo Rossetti
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Potentiometric Detection

An Automated Detection System for Most L-α-Amino Acids

1969 ◽  
Vol 15 (2) ◽  
pp. 154-161 ◽  
Author(s):  
K Van Dyke ◽  
C Szustkiewicz
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Detection System ◽  
Automated System ◽  
Acid Oxidase ◽  
Enzymatic Reactions ◽  
Amino Acid Oxidase ◽  
Automated Method ◽  
Wide Range

Abstract An automated system for the determination of the L-α form of the majority of amino acids is presented. The method is based upon oxidative deamination of the amino acid coupled with oxidation of o-dianisidine by hydrogen peroxide. This procedure can be used comparatively for the determination of a mixture of L-α-amino acids or for the majority of separated L-α-amino acids (especially in conjunction with column separations from urine and blood which give falsely positive identification with ninhydrin detection). The stereospecific nature of the L-α-amino acid oxidase enables the investigator to quantitate the amount of L-α-amino acid in the presence of the D-α form. From an academic viewpoint, the extreme sensitivity and wide range of the detection system make it advantageous for the study of the enzyme itself. This automated method also may be employed to follow enzymatic reactions—e.g., those catalyzed by peptidases or racemases. The methodology is extremely convenient with good reagent stability and is much more sensitive than manometric technics.

scholarly journals Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts

2005 ◽  
Vol 28 (9) ◽  
pp. 1578-1584 ◽  
Author(s):  
Kenji Hamase ◽  
Ryuichi Konno ◽  
Akiko Morikawa ◽  
Kiyoshi Zaitsu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Oxidase Activity ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Sensitive Determination

Determination of L-Amino Acids and L-Amino Acid Oxidase Activity Using Luminol Chemiluminescence

1977 ◽  
Vol 10 (12) ◽  
pp. 931-943 ◽  
Author(s):  
Stephen N. Lowery ◽  
Peter W. Carr ◽  
W. Rudolf Seitz
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Oxidase Activity ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Luminol Chemiluminescence

scholarly journals Biological role of D-amino acid oxidase and D-aspartate oxidase. Effects of D-amino acids.

1993 ◽  
Vol 268 (36) ◽  
pp. 26941-26949
Author(s):  
A D'Aniello ◽  
G D'Onofrio ◽  
M Pischetola ◽  
G D'Aniello ◽  
A Vetere ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Biological Role ◽  
Acid Oxidase ◽  
Amino Acid Oxidase
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