scholarly journals An efficient shoot regeneration system for medicinally important Elephantopus scaber Linn.

2015 ◽  
Vol 15 (2) ◽  
pp. 94-99 ◽  
Author(s):  
Jyothi Abraham ◽  
T. Dennis Thomas
Keyword(s):  
Age Groups ◽  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Shoot Induction ◽  
Regeneration System ◽  
Multiple Shoot Induction ◽  
Elephantopus Scaber ◽  
Cotyledonary Node Explants

An efficient protocol for the rapid micropropagation of medicinally important Elephantopus scaber has been standardized using cotyledonary node explants. Direct multiple shoot induction was observed when the cotyledonary node explants at various age groups were cultured on MS medium supplemented with various plant growth regulators. The highest shoot induction was obtained when the cotyledonary node explants from 20-day-old seedlings were cultured on MS medium supplemented with 1.5 mg L-1 TDZ and 0.5 mg L-1 NAA. On this medium, 98% of the cultures responded, with an average number of 33.7 shoots per explant. The highest frequency of rooting (100%) and mean number of roots (3.3 per shoot) were observed when the shoots were transferred to MS medium supplemented with 1.0 mg L-1 IBA. The plantlets raised in vitro were acclimatized and transferred to soil with a 92% success rate. The protocol described here may be utilized for multiplication and conservation of elite clones of E. scaber.

scholarly journals An efficient protocol for rapid plant regeneration from deembryonated cotyledons of black gram [Vigna mungo (L.) Hepper]

2019 ◽  
Author(s):  
R. Anandan ◽  
T. Deenathayalan ◽  
R. Bhuvaneshwari ◽  
M. Merlin Monisha ◽  
M. Prakash
Keyword(s):  
Shoot Elongation ◽  
Multiple Shoot ◽  
Vigna Mungo ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Black Gram ◽  
Regeneration System ◽  
Direct Shoot Regeneration ◽  
Multiple Shoot Induction

Here an efficient protocol for micropropagation of black gram [Vigna mungo (L.) Hepper] cv. VBN 3 is reported. The deembryonated cotyledonary explants were cultured on MS medium containing different concentrations of plant growth regulators. The maximum frequency (72%) of direct shoot regeneration (devoid of callus phase), multiple shoot induction and shoot elongation was achieved from culturing the explants on MS medium containing 3.0 mg/l of 6-benzylaminopurine (BAP). Up to 65% of the regenerated shoots were rooted on MS medium containing 0.25 mg/l of á-naphthalene acetic acid (NAA) within 3 weeks after subculturing. The in vitro-raised plantlets were successfully hardened first under culture room conditions with 62% survival rate and then in greenhouse. The identified regeneration system could be efficiently used in various in vitro manipulation studies in black gram as well.

scholarly journals Rapid in vitro micropropagation of Cicer arietinum L.

2008 ◽  
Vol 34 (No. 1) ◽  
pp. 1-5 ◽  
Author(s):  
G. Sujatha ◽  
N. Jayabalan ◽  
D. Ranjitha Kumari B
Keyword(s):  
Cicer Arietinum ◽  
Multiple Shoots ◽  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Indole Butyric Acid ◽  
Shoot Number

A rapid, simple and efficient protocol for <i>in vitro</i> multiple shoot induction and plantlet regeneration was achieved from three different explants of <i>Cicer arietinum</i>. The explants viz shoot tip, cotyledonary node and node were cultured on MS medium fortified with Benzyl Adenine (BA) (0.44&minus;8.88&mu;M) for multiple shoot induction. Multiple shoots proliferation was best observed at 4.44&mu;M BA from all the three explants within two weeks of culture. Of the three different explants tested, cotyledonary nodes produced the maximum number of shoots. Shoot number per explant ranged between 7 and 15. Individual shoots were aseptically excised and subcultured in the same media for shoot elongation. The elongated shoots were transferred to Indole Butyric Acid (IBA) (2.46&minus;12.30&mu;M) for root induction. Rooting was observed within two weeks of culture. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 76.3%. Plants looked healthy with no visually detectable phenotypic variations.

Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Rupesh S. Badere ◽  
Pallavi K. Rinkey
Keyword(s):  
Plant Growth Regulator ◽  
Gamma Ray ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Induction ◽  
Ethyl Methane Sulphonate ◽  
Multiple Shoot Induction ◽  
Methane Sulphonate ◽  
Regeneration Efficiency

The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

scholarly journals STANDARDIZATION OF MEDIUM FOR SYNTHETIC SEED GERMINATION OF SALVIA SCLAREA L.

2015 ◽  
Vol 2 (1) ◽  
pp. 118-120
Author(s):  
Durgha H ◽  
Ramya G ◽  
Gogul Ramanth M ◽  
Thirugnanasampandan R
Keyword(s):  
Seed Germination ◽  
Sodium Alginate ◽  
Calcium Chloride ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Synthetic Seed ◽  
Ms Medium ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Salvia Sclarea

Young nodal explants (0.5-1cm) of Salvia sclarea L. was used for synthetic seed preparation.Synthetic seeds were prepared using 5% sodium alginate and 1.11% calcium chloride. Seed germination was observed on MS medium fortified with 1.4µM GA3+4.4µM BA after twenty days of culture. Further multiple shoot induction was observed after fifteen days of shootinduction.

scholarly journals In vitro regeneration protocol of Rauvolfia serpentina L.

2018 ◽  
Vol 53 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S Khan ◽  
TA Banu ◽  
S Akter ◽  
B Goswami ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Number Of Shoots

An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018

In vitro propagation and multiple shoot induction of Rhodiola rosea L. by axillary bud culture

2012 ◽  
Vol 39 (2) ◽  
pp. 114-120 ◽  
Author(s):  
Kee-Hwa Bae ◽  
Myung-Suk Ko ◽  
Nam-Young Kim ◽  
Jae-Mo Song ◽  
Gwan-Pil Song
Keyword(s):  
In Vitro Propagation ◽  
Multiple Shoot ◽  
Rhodiola Rosea ◽  
Axillary Bud ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Axillary Bud Culture
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