Local Adaptation and Genetics of Acid-Stress Tolerance in the Moor Frog, Rana arvalis

Geographic variation indicating local adaptation, as well as its quantitative genetic basis, is commonly investigated in common garden experiments in the laboratory. However, the applicability of laboratory results to the complex conditions experienced by populations in the wild may be limited. Our previous laboratory experiments showed maternally determined local adaptation in embryonic acid-stress tolerance (viz. survival) of the moor frog, Rana arvalis Nilsson, 1842. Here we tested whether this laboratory finding holds even when embryos are exposed to acid stress in the wild. We conducted reciprocal crosses between an acid-origin population and a neutral-origin population of R. arvalis and transplanted the embryos to an acid site (pH ~4) in the field. Embryonic survival was much lower in the field experiment than in previous laboratory experiments, but, consistent with laboratory work, embryos from acid-origin females had threefold higher survival than embryos from neutral-origin females. These results suggest that laboratory tests can provide appropriate estimates of among population variation, as well as the quantitative genetic basis of acid-stress tolerance in amphibians.

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An Overview of the Helminths of Moor Frog Rana arvalis Nilsson, 1842 (Amphibia: Anura) in the Volga Basin

Diversity ◽

10.3390/d13020061 ◽

2021 ◽

Vol 13 (2) ◽

pp. 61

Author(s):

Igor V. Chikhlyaev ◽

Alexander B. Ruchin

Keyword(s):

Systematic Position ◽

Helminth Fauna ◽

Helminth Species ◽

Volga Basin ◽

Rana Arvalis ◽

Amphibian Species ◽

Volga River ◽

Spirometra Erinacei ◽

First Time ◽

Moor Frog

This is the first review of the helminth fauna of the moor frog Rana arvalis Nilsson, 1842 from the Volga river basin (Russia). The article summarizes the authors’ and literature data on the helminthic fauna of this species. The method of complete helminthological dissection was used. Thirthy-eight helminth species were recorded from three classes: Cestoda (1), Trematoda (28), and Chromadorea (9). Nine helminth species are new to the moor frog in Russia: trematodes Gorgodera varsoviensis Sinitzin, 1905, Strigea falconis Szidat, 1928, larvae, Neodiplostomum spathoides Dubois, 1937, larvae, Tylodelphys excavata (Rudolphi, 1803), larvae, Pharyngostomum cordatum (Diesing, 1850), larvae, Astiotrema monticelli Stossich, 1904, larvae and Encyclometra colubrimurorum (Rudolphi, 1819), larvae, nematodes Strongyloides spiralis Grabda-Kazubska, 1978 and Icosiella neglecta (Diesing, 1851). The cestode Spirometra erinacei (Rudolphi, 1918), larvae were observed of this amphibian species in the Volga basin for the first time. The nematodes Rhabdias bufonis, Oswaldocruzia filiformis, Cosmocerca ornata and the trematode Haplometra cylindracea form the core of the helminth fauna of the moor frog. Information on species of helminths includes systematic position, localization, areas of detection, type and scheme of life cycle, geographical distribution, and degree of specificity to host amphibians.

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The two-component system ActJK is involved in acid stress tolerance and symbiosis in Sinorhizobium meliloti

Journal of Biotechnology ◽

10.1016/j.jbiotec.2021.01.006 ◽

2021 ◽

Vol 329 ◽

pp. 80-91

Author(s):

Francisco J. Albicoro ◽

Walter O. Draghi ◽

María C. Martini ◽

María E. Salas ◽

G.A. Torres Tejerizo ◽

...

Keyword(s):

Stress Tolerance ◽

Sinorhizobium Meliloti ◽

Acid Stress ◽

Two Component System ◽

Component System ◽

Two Component

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Contribution of dps to Acid Stress Tolerance and Oxidative Stress Tolerance in Escherichia coli O157:H7

Applied and Environmental Microbiology ◽

10.1128/aem.66.9.3911-3916.2000 ◽

2000 ◽

Vol 66 (9) ◽

pp. 3911-3916 ◽

Cited By ~ 100

Author(s):

Sang Ho Choi ◽

David J. Baumler ◽

Charles W. Kaspar

Keyword(s):

Oxidative Stress ◽

Escherichia Coli ◽

Hydrogen Peroxide ◽

Stationary Phase ◽

Stress Tolerance ◽

Parent Strain ◽

Acid Stress ◽

Acid Tolerance ◽

Gastric Fluid ◽

Acid Challenge

ABSTRACT An Escherichia coli O157:H7dps::nptI mutant (FRIK 47991) was generated, and its survival was compared to that of the parent in HCl (synthetic gastric fluid, pH 1.8) and hydrogen peroxide (15 mM) challenges. The survival of the mutant in log phase (5-h culture) was significantly impaired (4-log10-CFU/ml reduction) compared to that of the parent strain (ca. 1.0-log10-CFU/ml reduction) after a standard 3-h acid challenge. Early-stationary-phase cells (12-h culture) of the mutant decreased by ca. 4 log10CFU/ml while the parent strain decreased by approximately 2 log10 CFU/ml. No significant differences in the survival of late-stationary-phase cells (24-h culture) between the parent strain and the mutant were observed, although numbers of the parent strain declined less in the initial 1 h of acid challenge. FRIK 47991 was more sensitive to hydrogen peroxide challenge than was the parent strain, although survival improved in stationary phase. Complementation of the mutant with a functional dps gene restored acid and hydrogen peroxide tolerance to levels equal to or greater than those exhibited by the parent strain. These results demonstrate that decreases in survival were from the absence of Dps or a protein regulated by Dps. The results from this study establish that Dps contributes to acid tolerance in E. coli O157:H7 and confirm the importance of Dps in oxidative stress protection.

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