scholarly journals The Change of Bacterial Spectrum after Storage of X. campestris pv. campestris Inoculated Cabbage Heads (Brassica oleracea var. capitata L.)

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 443 ◽  
Author(s):  
Lucia Ragasová ◽  
Eliška Peňázová ◽  
Filip Gazdík ◽  
Jakub Pečenka ◽  
Jana Čechová ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Brassica Oleracea ◽  
Storage Conditions ◽  
Bacillus Species ◽  
Pseudomonas Sp ◽  
Bacterial Cultures ◽  
Bacterial Spectrum ◽  
Minor Part ◽  
Cruciferous Plants ◽  
Subtilis Group

Changes in the bacterial spectrum of cabbage heads after storage under commonly used storage conditions were examined in this study. Cabbage seeds (Brassica oleracea var. capitata L.) were artificially inoculated with X. campestris pv. campestris (Xcc), a serious pathogen of cruciferous plants causing black rot. Isolation of bacterial cultures from Xcc-inoculated and non-inoculated cabbage heads were carried out in two time points—at the day of harvest and after four months of storage. According to our previous research and literature reports, the most frequent genera of bacteria were chosen for PCR testing, i.e., Bacillus cereus group, Bacillus subtilis group, Pseudomonas sp., and X. campestris pv. campestris. A few of the obtained bacterial cultures were negative for the four above-mentioned species. In those, other bacteria were identified by 16S rRNA sequencing. In both Xcc-inoculated and non-inoculated cabbage heads, changes of the bacterial spectrum over time were observed. The severity of Xcc infection of heads increased after four months of storage. Bacillus species represented the most frequently occurring bacterial genus. The presence of the Bacillus subtilis group increased significantly after storage in non-inoculated cabbage heads. The minor part of the other genera identified by sequencing in the first sampling were not detected in the stored cabbage heads. This was associated with a possible antagonistic behavior of Pseudomonas sp. and Bacillus sp.

PRODUCTION OF CYTASES ACTIVE ON BARLEY GUM BY BACTERIA OF THE GENUS BACILLUS

1953 ◽  
Vol 31 (1) ◽  
pp. 28-32 ◽  
Author(s):  
A. C. Blackwood
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Nitrogen Source ◽  
Freeze Drying ◽  
Shake Flask ◽  
Sole Nitrogen Source ◽  
Genus Bacillus ◽  
Original Activity ◽  
Bacterial Cultures ◽  
Bacillus Genus

One hundred and fourteen bacterial cultures representing most of the species in the Bacillus genus were tested for the production of extracellular barley gum cytase. Assays were made on shake-flask cultures grown on a medium containing glucose and yeast extract. Although all the organisms had some enzymatic activity, certain strains of Bacillus subtilis gave the best yields of cytase. On a medium with asparagine as the sole nitrogen source even higher yields were obtained. The crude cytase preparations were stable and after freeze-drying most of the original activity remained.

Raman microspectroscopy as an identification tool within the phylogenetically homogeneous ‘Bacillus subtilis’-group

2006 ◽  
Vol 29 (8) ◽  
pp. 650-660 ◽  
Author(s):  
Didier Hutsebaut ◽  
Joachim Vandroemme ◽  
Jeroen Heyrman ◽  
Peter Dawyndt ◽  
Peter Vandenabeele ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Raman Microspectroscopy ◽  
Subtilis Group ◽  
Identification Tool

Two distinct groups within the Bacillus subtilis group display significantly different spore heat resistance properties

2015 ◽  
Vol 45 ◽  
pp. 18-25 ◽  
Author(s):  
Erwin M. Berendsen ◽  
Marcel H. Zwietering ◽  
Oscar P. Kuipers ◽  
Marjon H.J. Wells-Bennik
Keyword(s):  
Bacillus Subtilis ◽  
Heat Resistance ◽  
Subtilis Group

Evaluation of Anaerobic Growth of Bacillus licheniformis and Bacillus subtilis in Tomato Juice

1992 ◽  
Vol 55 (9) ◽  
pp. 672-677 ◽  
Author(s):  
J. H. RODRIGUEZ ◽  
M. A. COUSIN ◽  
P. E. NELSON
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Clostridium Botulinum ◽  
Tomato Juice ◽  
Anaerobic Growth ◽  
Bacillus Species ◽  
Potential Hazard ◽  
Anaerobic Conditions ◽  
Bacillus Strains ◽  
Ph Elevation

Conditions responsible for growth and pH elevation by selected strains of Bacillus subtilis and Bacillus licheniformis were studied in order to assess the potential hazard of metabiosis occurring between Clostridium botulinum and mesophilic Bacillus species in aseptically packaged tomato juice. The effects of the initial tomato juice pH on the growth of these Bacillus strains were evaluated. Cultures of B. licheniformis previously identified by Fields et al. (6) were reclassified as B. subtilis because none grew under strict anaerobic conditions nor used propionate. B. subtilis did not grow under strict anaerobic conditions but could use oxygen if present in the environment. None of the B. subtilis or B. licheniformis strains grew at pH 4.0 or 4.2. Only B. subtilis 075-T-09 and B. licheniformis 64-83-46 strains isolated from acidic foods were able to grow at pH 4.4 in tomato juice. Anaerobically, B. licheniformis strains did not grow in tomato juice or tomato juice with added nitrate and thiamine and even showed a loss of viability. Both B. licheniformis and B. subtilis require oxygen for growth in tomato juice at pH 4.4.

scholarly journals THE SOURCE OF THE MICROORGANISMS IN THE LUNGS OF NORMAL ANIMALS

1922 ◽  
Vol 36 (3) ◽  
pp. 317-328 ◽  
Author(s):  
F. S. Jones
Keyword(s):  
Bacillus Subtilis ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
White Mouse ◽  
Lymph Glands ◽  
Dry Food ◽  
Subtilis Group

It has been possible to show that the lungs of such animals as the calf, rabbit, guinea pig, white rat, and white mouse are readily invaded by organisms. The most frequent types observed in cultures from the border of the lungs have been streptothrix, molds, and bacteria of the Bacillus subtilis group. These forms originate in certain dry food stuffs (hay and straw). By withholding or moistening these materials it has been possible to diminish the number of organisms in the lungs. When these materials have been supplied to mice whose lungs under usual conditions contain only a few organisms, the number of positive cultures increases and is comparable with those of the larger animals. The bronchial lymph glands of all guinea pigs examined developed, in 66⅔ per cent of the tubes, organisms similar to those obtained from the lungs.

Morphological and molecular based identification of Antifungal bacillus licheniformis

2017 ◽  
Vol 17 (1) ◽  
pp. 31-35
Author(s):  
B Oyuntogtokh ◽  
M Byambasuren
Keyword(s):  
Bacillus Licheniformis ◽  
Fungal Pathogens ◽  
Crop Production ◽  
Plant Pathogens ◽  
Control Plant ◽  
Plant Diseases ◽  
Bacillus Species ◽  
Bacterial Strains ◽  
Fusarium Spp ◽  
Bacterial Cultures

At present, plant diseases caused by soil borne plant pathogens have major constraints on crop production. Which include genera Fusarium spp, Phytophtora spp, Sclerotinia and Altenaria. Due to this reason, chemical fungicides are routinely used to control plant disease, which is also true in Mongolian case. However, use of these chemicals has caused various problems including environmental pollution with consequence of toxicity to human health also resistance of some pathogens to these fungicides are present. Fortunately, an alternative method to reduce the effect of these plant pathogens is the use of antagonist microorganisms. Therefore, some species of the genus Bacillus are recognized as one of the most effective biological control agent.Our research was focused to isolate Bacillus licheniformis, with antifungal potential, from indigenous sources. In the current study, 28 bacterial cultures were isolated from soil and fermented mare’s milk also named as koumiss. Isolated bacterial cultures were identified according to simplified key for the tentative identification of typical strain of Bacillus species. As a result 8 strains were positive and further screened for antifungal activity against Fusarium spp and Alternaria solani. Out of these 8 strains 5 strains are selected based on their high effectiveness against fungal pathogens and for further confirmation Polymerase Chain reaction run for effective bacterial strains using specific primers B.Lich-f and B.Lich-r. 

scholarly journals Panophthalmitis due to an Organism of the Bacillus Subtilis Group

1952 ◽  
Vol 36 (7) ◽  
pp. 389-392 ◽  
Author(s):  
R. Davenport ◽  
C. Smith
Keyword(s):  
Bacillus Subtilis ◽  
Subtilis Group
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