Photosynthesis and Activity of Phosphoenolpyruvate carboxylase in Nicotiana tabacum L. Leaves Infected by Potato virus A and Potato virus Y

The recessive resistance genes pot-1 and pvr2 in Lycopersicon hirsutum and Capsicum annuum, respectively, control Potato virus Y (PVY) accumulation in the inoculated leaves. Infectious cDNA molecules from two PVY isolates differing in their virulence toward these resistances were obtained using two different strategies. Chimeras constructed with these cDNA clones showed that a single nucleotide change corresponding to an amino acid substitution (Arg119His) in the central part of the viral protein genome-linked (VPg) was involved in virulence toward the pot-1 resistance. On the other hand, 15 nucleotide changes corresponding to five putative amino acid differences in the same region of the VPg affected virulence toward the pvr21 and pvr22 resistances. Substitution models identified six and five codons within the central and C terminal parts of the VPg for PVY and for the related potyvirus Potato virus A, respectively, which undergo positive selection. This suggests that the role of the VPg-encoding region is determined by the protein and not by the viral RNA apart from its protein-encoding capacity.

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Dynamic expression analysis of early response genes induced by potato virus Y in PVY-resistant Nicotiana tabacum

Plant Cell Reports ◽

10.1007/s00299-016-2080-1 ◽

2016 ◽

Vol 36 (2) ◽

pp. 297-311 ◽

Cited By ~ 6

Author(s):

Shuai Chen ◽

Fengxia Li ◽

Dan Liu ◽

Caihong Jiang ◽

Lijie Cui ◽

...

Keyword(s):

Nicotiana Tabacum ◽

Expression Analysis ◽

Potato Virus ◽

Potato Virus Y ◽

Early Response ◽

Dynamic Expression ◽

Early Response Genes

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Transfer of resistance to potato virus�Y (PVY) from Nicotiana africana to Nicotiana tabacum: possible influence of tissue culture on the rate of introgression

Theoretical and Applied Genetics ◽

10.1007/s00122-004-1893-4 ◽

2005 ◽

Vol 110 (4) ◽

pp. 678-687 ◽

Cited By ~ 16

Author(s):

R. S. Lewis

Keyword(s):

Tissue Culture ◽

Nicotiana Tabacum ◽

Potato Virus ◽

Potato Virus Y ◽

Nicotiana Africana

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Coat protein gene-mediated resistance to Potato virus A in transgenic plants is suppressed following infection with another potyvirus

Journal of General Virology ◽

10.1099/0022-1317-82-9-2275 ◽

2001 ◽

Vol 82 (9) ◽

pp. 2275-2278 ◽

Cited By ~ 33

Author(s):

E. I. Savenkov ◽

J. P. T. Valkonen

Keyword(s):

Steady State ◽

Coat Protein ◽

Transgenic Plants ◽

Potato Virus ◽

Potato Virus Y ◽

Protein Gene ◽

Specific Resistance ◽

Virus Genome ◽

Potato Virus A ◽

Steady State Levels

High levels of resistance to Potato virus A (PVA, genus Potyvirus), indicated by absence of detectable infection in inoculated leaves, were attained in Nicotiana benthamiana transformed with a construct expressing the PVA 5′-untranslated region fused with the coat protein (CP)-encoding sequence. Low steady-state levels of the transgene transcripts were detected. Resistance was PVA-specific and did not protect the plants against infection with Potato virus Y (PVY, genus Potyvirus). Consequently, the steady-state levels of the CP-transgene mRNA were greatly elevated in the plants infected with PVY, and plants became susceptible to infection with PVA. These data show that virus resistance obtained by expressing regions of a plant virus genome in transgenic plants may be suppressed following infection with another virus that evades the virus-specific resistance.

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