scholarly journals Mutations in Potato virus Y Genome-Linked Protein Determine Virulence Toward Recessive Resistances in Capsicum annuum and Lycopersicon hirsutum

2004 ◽  
Vol 17 (3) ◽  
pp. 322-329 ◽  
Author(s):  
Benoît Moury ◽  
Caroline Morel ◽  
Elisabeth Johansen ◽  
Laurent Guilbaud ◽  
Sylvie Souche ◽  
...  
Keyword(s):  
Amino Acid ◽  
Capsicum Annuum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Cdna Clones ◽  
Lycopersicon Hirsutum ◽  
Potato Virus A ◽  
Single Nucleotide Change ◽  
Protein Encoding

The recessive resistance genes pot-1 and pvr2 in Lycopersicon hirsutum and Capsicum annuum, respectively, control Potato virus Y (PVY) accumulation in the inoculated leaves. Infectious cDNA molecules from two PVY isolates differing in their virulence toward these resistances were obtained using two different strategies. Chimeras constructed with these cDNA clones showed that a single nucleotide change corresponding to an amino acid substitution (Arg119His) in the central part of the viral protein genome-linked (VPg) was involved in virulence toward the pot-1 resistance. On the other hand, 15 nucleotide changes corresponding to five putative amino acid differences in the same region of the VPg affected virulence toward the pvr21 and pvr22 resistances. Substitution models identified six and five codons within the central and C terminal parts of the VPg for PVY and for the related potyvirus Potato virus A, respectively, which undergo positive selection. This suggests that the role of the VPg-encoding region is determined by the protein and not by the viral RNA apart from its protein-encoding capacity.

scholarly journals The 6K2 Protein and the VPg of Potato Virus A Are Determinants of Systemic Infection in Nicandra physaloides

1999 ◽  
Vol 12 (12) ◽  
pp. 1074-1081 ◽  
Author(s):  
Minna-Liisa Rajamäki ◽  
Jari P. T. Valkonen
Keyword(s):  
Amino Acid ◽  
Potato Virus ◽  
Viral Genome ◽  
Recombinant Virus ◽  
Systemic Infection ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Cdna Clones ◽  
Post Inoculation ◽  
Potato Virus A

Infection with the isolate PVA-M of potato virus A (PVA; genus Potyvirus) is restricted to the inoculated leaves of Nicandra physaloides (Solanaceae), whereas the isolate PVA-B11 infects plants systemically by 10 days post inoculation. Resistance to systemic infection was shown to develop during plant growth. A recombinant virus (B11-M) in which a 1,208-nucleotide sequence of the full-length cDNA clone of PVA-B11 was replaced with the corresponding sequence from PVA-M displayed a phenotype similar to that of PVA-M. The replaced sequence contained four amino acid differences between the two isolates: one in the 6K2 protein and three in the viral genome-linked protein (VPg). Site-directed mutagenesis of the cDNA clones and inoculation of the mutants to N. physaloides indicated that the amino acid substitutions of Met5Val in the 6K2 protein or Leu185Ser in the VPg permitted vascular movement and systemic infection. However, resistance was only partially overcome by these changes, since systemic infection proceeded at a slower rate than with PVA-B11. The amino acid substitution Val116Met in the VPg alone was sufficient to overcome resistance and recover the phenotype of the isolate PVA-B11. These data indicated that both the 6K2 protein and the VPg were avirulence determinants of PVA-M in N. physaloides and suggested a possibly coordinated function of them in the vascular movement of PVA.

scholarly journals Reação de progênies de pimentão ao Potato Virus Y

Bragantia ◽  
2003 ◽  
Vol 62 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Humberto Sacchi ◽  
Arlete Marchi Tavares de Melo ◽  
Addolorata Colariccio
Keyword(s):  
Capsicum Annuum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Nicotiana Glutinosa ◽  
Capsicum Annuum L

Este trabalho teve como metas avaliar a reação de progênies de pimentão (Capsicum annuum L.) a um isolado de Potato virus Y (PVY), coletado em plantas de pimentão cv. Magda, em Paulínia (SP) e identificar esse isolado. O isolado foi identificado por meio de testes biológicos de inoculação mecânica em plantas indicadoras, testes sorológicos (TAS-ELISA) empregando-se anticorpos monoclonais para as estirpes, comum (PVY0), necrótica (PVY N ) e clorótica (PVY C). Trinta e cinco progênies de pimentão do programa de melhoramento do IAC, foram inoculadas com este isolado denominado PVY-Pa, e cinco cultivares comerciais foram inoculadas como controle positivo, pois têm uma reação conhecida ao PVY. O delineamento dos experimentos foi inteiramente casualizado. Os resultados dos testes biológicos e as observações em preparações de contrastação negativa evidenciaram a existência de uma infecção mista, por potivirus e tobamovirus, nas amostras provenientes de Paulínia. O PVY foi isolado de Nicotiana glutinosa com infecção sistêmica, nas quais, foram observadas inclusões cilíndricas do tipo cata-ventos, características da família Potyviridae, em cortes ultrafinos de células do mesófilo. Em TAS-ELISA, plantas de N. glutinosa, infectadas, apresentaram reação negativa para os anticorpos empregados. Porém, a ausência de sintomas em 'Myr 10' e 'Myr 29', indicou tratar-se da estirpe PVYm. A avaliação das progênies inoculadas foi feita pela utilização da taxa da proporção entre plantas com ausência e presença de sintomas de PVY. Das progênies avaliadas sete progênies F3 derivadas de híbridos triplos de pimentão apresentaram plantas com ausência de sintomas, das quais o vírus não pode ser recuperado pela inoculação em N. glutinosa, confirmando a ausência de multiplicação do vírus nestes híbridos.

scholarly journals YIELD AND TOLERANCE OF BELL PEPPER (CAPSICUM ANNUUM L.) CULTIVARS TO POTATO VIRUS Y ISOLATE FROM PUERTO RICO

1969 ◽  
Vol 71 (3) ◽  
pp. 337-340
Author(s):  
Jaime Escudero ◽  
Guillermo Fornaris-Rullán ◽  
Elvin Caraballo
Keyword(s):  
Puerto Rico ◽  
Capsicum Annuum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Bell Pepper ◽  
Capsicum Annuum L

YIELD AND TOLERANCE OF BELL PEPPER (CAPSICUM ANNUUM L.) CULTIVARS TO POTATO VIRUS Y ISOLATE FROM PUERTO RICO

scholarly journals Role of the methionine cycle in the temperature‐sensitive responses of potato plants to potato virus Y

2020 ◽  
Vol 22 (1) ◽  
pp. 77-91 ◽  
Author(s):  
Igor Fesenko ◽  
Nadezhda Spechenkova ◽  
Anna Mamaeva ◽  
Antonida V. Makhotenko ◽  
Andrew J. Love ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Temperature Sensitive ◽  
Potato Plants ◽  
Methionine Cycle

Role of Hairy Nightshade in the Transmission of Different Potato virus Y Strains on Solanum tuberosum (L.)

2010 ◽  
Vol 11 (1) ◽  
pp. 38 ◽  
Author(s):  
Felix A. Cervantes ◽  
Juan M. Alvarez
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Green Peach Aphid ◽  
Transmission Rate ◽  
Solanum Tuberosum L ◽  
Macrosiphum Euphorbiae ◽  
Invasive Weed ◽  
Potato Plants ◽  
Tuber Necrosis

The complexity of the Potato virus Y (PVY) (Potyviridae: Potyvirus) pathosystem is affected by the presence of several virus strains that differ in their ability to produce tuber necrosis and by the presence of an alternate host that could increase the amount of inoculum in potato fields. Solanum sarrachoides (Sendtner) is an invasive weed from South America present in Pacific Northwest potato agro-ecosystems. It serves as reservoir of PVY and its most efficient vectors: the green peach aphid, Myzus persicae (Sulzer), and the potato aphid, Macrosiphum euphorbiae (Thomas). The role of S. sarracoides as vector and virus reservoir in PVY epidemiology was investigated through a series of laboratory and greenhouse experiments. We studied the symptoms produced in S. sarracoides upon infection with necrotic and non-necrotic strains of PVY and looked at the percentage of infection and titer accumulation of these strains. PVY infection in S. sarrachoides produced symptoms similar to those produced in PVY-infected potato plants. Mottling and yellowing were the main symptoms of infection observed in S. sarrachoides plants, especially by PVYO and PVYNTN infection. Greenhouse transmission studies revealed that PVY-infected S. sarrachoides increased the transmission rate of PVY necrotic strains by M. persicae. The necrotic strain PVYNTN reached higher titer in S. sarrachoides than in potato plants when compared to PVYO and PVYN:O These findings have broadened our understanding of the role and importance of S. sarrachoides in the PVY epidemiology in the potato ecosystems and could potentially be included in the development or optimization of virus management programs. Accepted for publication 15 March 2010. Published 26 May 2010.

scholarly journals Different Mutations in the Genome-Linked Protein VPg of Potato virus Y Confer Virulence on the pvr23 Resistance in Pepper

2006 ◽  
Vol 19 (5) ◽  
pp. 557-563 ◽  
Author(s):  
Valérie Ayme ◽  
Sylvie Souche ◽  
Carole Caranta ◽  
Mireille Jacquemond ◽  
Joël Chadœuf ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genetic Drift ◽  
Potato Virus ◽  
High Frequency ◽  
Potato Virus Y ◽  
Relative Fitness ◽  
Amino Acid Substitutions ◽  
Nucleotide Substitutions ◽  
Single Nucleotide ◽  
Pepper Plants

Five different amino acid substitutions in the VPg of Potato virus Y were shown to be independently responsible for virulence toward pvr23 resistance gene of pepper. A consequence of these multiple mutations toward virulence involving single nucleotide substitutions is a particularly high frequency of resistance breaking (37% of inoculated plants from the first inoculation) and suggests a potentially low durability of pvr23 resistance. These five mutants were observed with significantly different frequencies, one of them being overrepresented. Genetic drift alone could not explain the observed distribution of virulent mutants. More plausible scenarios were obtained by taking into account either the relative substitution rates, the relative fitness of the mutants in pvr23 pepper plants, or both.

scholarly journals Mutational Analysis of the Amino Acid Residues Essential for thecisandtransCleavage Activity of the Potato Virus Y 50-kDa Protease

1993 ◽  
Vol 57 (9) ◽  
pp. 1536-1540 ◽  
Author(s):  
Yukiko Yoshida ◽  
Makoto Hidaka ◽  
Haruhiko Masaki ◽  
Takeshi Uozumi
Keyword(s):  
Amino Acid ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Mutational Analysis ◽  
Amino Acid Residues
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