Intraspecific and Interspecific Variation of the Mitochondrial Gene of Cytochrome Oxidase I in Ladybirds (Coleoptera: Coccinellidae)

The phylogenetic relationships among 32 individuals of Australian freshwater crayfish belonging to the Cherax destructor-complex were investigated using a dataset comprising sequences from four mitochondrial gene regions: the large subunit rRNA (16S rRNA), cytochrome oxidase I (COI), adenosine triphosphatase 6 (ATPase 6), and cytochrome oxidase III (COIII). A total of 1602 bp was obtained, and a combined analysis of the data produced a tree with strong support (bootstrap values 94–100%) for three divergent lineages, verifying the phylogenetic hypotheses of relationships within the C. destructor species-complex suggested in previous studies. Overall, sequences from the 16S rRNA gene showed the least variation compared to those generated from protein coding genes, which presented considerably greater levels of divergence. The level of divergence within C. destructor was found to be greater than that observed in other species of freshwater crayfish, but interspecific variation among species examined in the present study was similar to that reported previously.

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Mitochondrial gene expression in small intestinal epithelial cells

Biochemical Journal ◽

10.1042/bj3080665 ◽

1995 ◽

Vol 308 (2) ◽

pp. 665-671 ◽

Cited By ~ 5

Author(s):

T P Mayall ◽

I Bjarnason ◽

U Y Khoo ◽

T J Peters ◽

A J S Macpherson

Keyword(s):

Epithelial Cells ◽

Cytochrome Oxidase ◽

Cytochrome B ◽

Intestinal Epithelial Cells ◽

Mitochondrial Gene ◽

Cytochrome Oxidase I ◽

Mrna Levels ◽

Intestinal Epithelial ◽

Mitochondrial Transcription Factor ◽

Small Intestinal

Most mitochondrial genes are transcribed as a single large transcript from the heavy strand of mitochondrial DNA, and are subsequently processed into the proximal mitochondrial (mt) 12 S and 16 S rRNAs, and the more distal tRNAs and mRNAs. We have shown that in intestinal epithelial biopsies the steady-state levels of mt 12 S and 16 S rRNA are an order of magnitude greater than those of mt mRNAs. Fractionation of rat small intestinal epithelial cells on the basis of their maturity has shown that the greatest ratios of 12 S mt rRNA/cytochrome b mt mRNA or 12 S mt rRNA/cytochrome oxidase I mt mRNA are found in the surface mature enterocytes, with a progressive decrease towards the crypt immature enteroblasts. Cytochrome b and cytochrome oxidase I mt mRNA levels are relatively uniform along the crypt-villus axis, but fractionation experiments showed increased levels in the crypt base. The levels of human mitochondrial transcription factor A are also greater in immature crypt enteroblasts compared with mature villus enterocytes. These results show that the relative levels of mt rRNA and mRNA are distinctly regulated in intestinal epithelial cells according to the crypt-villus position and differentiation status of the cells, and that there are higher mt mRNA and mt TFA levels in the crypts, consistent with increased transcriptional activity during mitochondrial biogenesis in the immature enteroblasts.

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Akemetopon, a new genus containing three new species of planthoppers (Hemiptera: Fulgoroidea: Delphacidae)

Zootaxa ◽

10.11646/zootaxa.3007.1.2 ◽

2011 ◽

Vol 3007 (1) ◽

pp. 50

Author(s):

KATHRYN M. WEGLARZ ◽

CHARLES R. BARTLETT

Keyword(s):

New Species ◽

Maximum Likelihood ◽

Cytochrome Oxidase ◽

New Genus ◽

Mitochondrial Gene ◽

Cytochrome Oxidase I ◽

Maximum Likelihood Analysis ◽

Key To Species ◽

Likelihood Analysis ◽

Three New Species

The new delphacid genus Akemetopon gen. n. (Delphacinae: Delphacini) is described and illustrated with 3 new species from Arizona and Mexico: Akemetopon inornatum sp. n., A. politum sp. n., and A. ainigma sp. n. A key to species is provided. The barcoding portion of the mitochondrial gene Cytochrome Oxidase I (COI) was sequenced for A. politum sp. n. A maximum likelihood analysis places this genus in the basal Delphacini.

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Phylogenetic Relationships in Some Species of the Genus Macrobrachium Based on Nucleotide Sequences of the Mitochondrial Gene Cytochrome Oxidase I

Modern Approaches to the Study of Crustacea ◽

10.1007/978-1-4615-0761-1_44 ◽

2002 ◽

pp. 319-322

Author(s):

Guido Pereira ◽

Hilda De Stefano ◽

Joseph Staton ◽

Brian Farrell

Keyword(s):

Cytochrome Oxidase ◽

Phylogenetic Relationships ◽

Mitochondrial Gene ◽

Nucleotide Sequences ◽

Cytochrome Oxidase I

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Notes on identity, new synonymy and larva of Ivalia Jacoby (Coleoptera: Chrysomelidae) with description of a new species

Zootaxa ◽

10.11646/zootaxa.1363.1.4 ◽

2006 ◽

Vol 1363 (1) ◽

pp. 49 ◽

Cited By ~ 6

Author(s):

CATHERINE N. DUCKETT ◽

K. D. PRATHAPAN ◽

ALEXANDER S. KONSTANTINOV

Keyword(s):

New Species ◽

Cytochrome Oxidase ◽

South India ◽

Mitochondrial Gene ◽

Cytochrome Oxidase I ◽

Larval Morphology ◽

Flea Beetles ◽

New Synonymy ◽

A New Species

Genus Ivalia Jacoby is characterized morphologically, and Amphimeloides Jacoby syn. nov. and Taizonia Chen syn. nov. are junior synonyms with it. Several Ivalia species are figured, including Ivalia bella (Chen) comb. nov., I. dorsalis (Jacoby) comb. nov., and I. viridipennis Jacoby. A new species of Ivalia from the Nilgiri Hills in south India, I. korakundah sp. nov., is described and illustrated, including the larvae. Larvae were associated with adults by sequencing a fragment of the mitochondrial gene cytochrome oxidase I. Larval morphology is discussed and compared with that of other flea beetles.

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Lepidochitona pseudoliozonis, una nueva especie de quitón (Polyplacophora: Ischnochitonidae) del norte del Caribe

Revista de Biología Tropical ◽

10.15517/rbt.v63i2.14862 ◽

2015 ◽

Vol 63 (2) ◽

pp. 369 ◽

Cited By ~ 1

Author(s):

Cedar I. García-Ríos

Keyword(s):

Puerto Rico ◽

New Species ◽

Cytochrome Oxidase ◽

Mitochondrial Gene ◽

Florida Keys ◽

Cytochrome Oxidase I ◽

Type Locality ◽

Gene Coding ◽

The Caribbean ◽

Morphological Differences

The genus Lepidochitona (Gray 1821) contains relatively small chitons with a distinctive girdle, dorsally clothed with non-overlapping calcareous corpuscles. In the Caribbean, it is represented by four species: L. liozonis (Dall, & Simpson, 1901), L. rosea Kaas, 1972, L. rufoi García-Ríos, 2010 and L. bullocki García-Ríos, 2011. A rutinary morphological inspection of 10 specimens of a Lepidochitona species from the Florida Keys was concordant with L. liozonis (the only species of the genus informed for Florida). They did not show many morphological differences that could justify its separation from the specimens from Puerto Rico (the type locality). However, the comparison of sequences of the mitochondrial gene coding for cytochrome oxidase I (COI) of L. liozonis from Puerto Rico and the Florida specimens showed a divergence of 14%. This divergence is incompatible with a reproductively connected species. In addition to their genetic differences, the new species differs from L. liozonis in having bigger size, longer marginal spicules and a postmucronal slope very concave.

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