Changes in water relations and in some physiological functions of bean under very light osmotic shock induced by polyethylene glycol

1992 ◽  
Vol 84 (4) ◽  
pp. 537-548
Author(s):  
Vittorio Raggi
Keyword(s):  
Polyethylene Glycol ◽  
Water Relations ◽  
Osmotic Shock ◽  
Physiological Functions

scholarly journals Oxygen Availability in Polyethylene Glycol Solutions and Its Implications in Plant-Water Relations

1975 ◽  
Vol 55 (1) ◽  
pp. 20-24 ◽  
Author(s):  
John Mexal ◽  
James T. Fisher ◽  
Janet Osteryoung ◽  
C. P. Patrick Reid
Keyword(s):  
Polyethylene Glycol ◽  
Water Relations ◽  
Oxygen Availability ◽  
Plant Water Relations ◽  
Plant Water

Impact of drought stress induced by polyethylene glycol on growth, water relations and cell viability of Norway spruce seedlings

2018 ◽  
Vol 25 (9) ◽  
pp. 8951-8962 ◽  
Author(s):  
Ilya E. Zlobin ◽  
Yury V. Ivanov ◽  
Alexander V. Kartashov ◽  
Vladimir V. Kuznetsov
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Cell Viability ◽  
Norway Spruce ◽  
Water Relations

Bacterial Water Relations Using Polyethylene Glycol 4000

1982 ◽  
Vol 46 (3) ◽  
pp. 542-547 ◽  
Author(s):  
K. J. McAneney ◽  
R. F. Harris ◽  
W. R. Gardner
Keyword(s):  
Polyethylene Glycol ◽  
Water Relations ◽  
Polyethylene Glycol 4000

Properties of Isolated Mitochondria of Agaricus Bisporus: Their Relationship to Dormancy and the Germination of Spores

1973 ◽  
Vol 31 ◽  
pp. 458-459
Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Microscopic Examination ◽  
Agaricus Bisporus ◽  
Osmotic Shock ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Isolated Mitochondria ◽  
Dna Strands ◽  
Cytoplasmic Ribosomes

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

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