scholarly journals Detection of circulating prostate-specific antigen-positive cells in patients with prostate cancer by flow cytometry and reverse transcription polymerase chain reaction

1996 ◽  
Vol 74 (3) ◽  
pp. 400-405 ◽  
Author(s):  
EJ Fadlon ◽  
RC Rees ◽  
C McIntyre ◽  
RM Sharrard ◽  
J Lawry ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Flow Cytometry ◽  
Polymerase Chain Reaction ◽  
Prostate Specific Antigen ◽  
Reverse Transcription ◽  
Specific Antigen ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of prostate-specific antigen mRNA and protein in breast tumors

1996 ◽  
Vol 42 (3) ◽  
pp. 361-366 ◽  
Author(s):  
N Zarghami ◽  
E P Diamandis
Keyword(s):  
Polymerase Chain Reaction ◽  
Prostate Specific Antigen ◽  
Reverse Transcription ◽  
Specific Antigen ◽  
Breast Tumors ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Polymerase Chain ◽  
Good Agreement

Abstract We have developed reverse transcription-polymerase chain reaction (RT- PCR) methods for detecting prostate-specific antigen (PSA) mRNA. Using these methods, and a highly sensitive immunofluorometric assay for measuring PSA protein, we have assessed the concentrations of PSA mRNA and PSA protein in 30 primary breast tumors and a few other control tissues. We found good agreement between presence of PSA protein and PSA mRNA in breast tumors. We thus propose that, in women, detection of PSA protein or PSA mRNA in tissues and tumors offers equivalent information. Because PSA protein is present in male blood and thus could contaminate extracts from tumors and tissues from men, we propose that the RT-PCR methods we describe be used to assess nonprostatic expression of the PSA gene in men.

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