scholarly journals Effect of expression of adenine phosphoribosyltransferase on the in vivo anti-tumor activity of prodrugs activated by E. coli purine nucleoside phosphorylase

2011 ◽  
Vol 18 (6) ◽  
pp. 390-398 ◽  
Author(s):  
W B Parker ◽  
P W Allan ◽  
W R Waud ◽  
J S Hong ◽  
E J Sorscher
Keyword(s):  
Purine Nucleoside Phosphorylase ◽  
Purine Nucleoside ◽  
Adenine Phosphoribosyltransferase ◽  
Nucleoside Phosphorylase ◽  
E Coli ◽  
Anti Tumor Activity

scholarly journals In vivo sensitization of ovarian tumors to chemotherapy by expression of E. coli purine nucleoside phosphorylase in a small fraction of cells

Gene Therapy ◽  
2000 ◽  
Vol 7 (20) ◽  
pp. 1738-1743 ◽  
Author(s):  
V K Gadi ◽  
S D Alexander ◽  
J E Kudlow ◽  
P Allan ◽  
W B Parker ◽  
...  
Keyword(s):  
Purine Nucleoside Phosphorylase ◽  
Ovarian Tumors ◽  
Purine Nucleoside ◽  
Nucleoside Phosphorylase ◽  
E Coli

scholarly journals Metabolism of purines in macrophages. Effect of functional state of the cells.

1980 ◽  
Vol 152 (1) ◽  
pp. 241-246 ◽  
Author(s):  
R J Soberman ◽  
M L Karnovsky
Keyword(s):  
Adenosine Deaminase ◽  
Purine Nucleoside Phosphorylase ◽  
Sodium Caseinate ◽  
Peritoneal Macrophages ◽  
Purine Nucleoside ◽  
Adenine Phosphoribosyltransferase ◽  
Nucleoside Phosphorylase ◽  
Mouse Macrophages ◽  
Different Populations

Ecto-5'-nucleotidase is known to be diminished markedly in activated compared to control mouse macrophages. The level of three purine nucleoside metabolizing enzymes, adenosine deaminase (EC 3.5.4.4), purine nucleoside phosphorylase (EC 2.4.2.1), and adenine phosphoribosyltransferase (EC 2.4.2.7) were measured in the sonicates of different populations of mouse peritoneal macrophages. Levels of adenine phosphoribosyltransferase and purine nucleoside phosphorylase in macrophages that were elicited with sodium caseinate or activated in vivo by prior intravenous injection of Listeria monocytogenes were eight times higher than those in resident cells. Levels of adenosine deaminase also tended to increase and were two times higher in elicited cells than in resident cells. The Km of each enzyme was the same in each cell population. The findings suggest that the levels of the ecto-5'-nucleotidase and of the intracellular enzymes are coordinated.

scholarly journals Radical Dehalogenation and Purine Nucleoside Phosphorylase E. coli: How Does an Admixture of 2′,3′-Anhydroinosine Hinder 2-fluoro-cordycepin Synthesis

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 539
Author(s):  
Alexey L. Kayushin ◽  
Julia A. Tokunova ◽  
Ilja V. Fateev ◽  
Alexandra O. Arnautova ◽  
Maria Ya. Berzina ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Chemical Synthesis ◽  
Purine Nucleoside Phosphorylase ◽  
Purine Nucleoside ◽  
Preparative Synthesis ◽  
Nucleoside Phosphorylase ◽  
E Coli ◽  
First Time

During the preparative synthesis of 2-fluorocordycepin from 2-fluoroadenosine and 3′-deoxyinosine catalyzed by E. coli purine nucleoside phosphorylase, a slowdown of the reaction and decrease of yield down to 5% were encountered. An unknown nucleoside was found in the reaction mixture and its structure was established. This nucleoside is formed from the admixture of 2′,3′-anhydroinosine, a byproduct in the preparation of 3-′deoxyinosine. Moreover, 2′,3′-anhydroinosine forms during radical dehalogenation of 9-(2′,5′-di-O-acetyl-3′-bromo- -3′-deoxyxylofuranosyl)hypoxanthine, a precursor of 3′-deoxyinosine in chemical synthesis. The products of 2′,3′-anhydroinosine hydrolysis inhibit the formation of 1-phospho-3-deoxyribose during the synthesis of 2-fluorocordycepin. The progress of 2′,3′-anhydroinosine hydrolysis was investigated. The reactions were performed in D2O instead of H2O; this allowed accumulating intermediate substances in sufficient quantities. Two intermediates were isolated and their structures were confirmed by mass and NMR spectroscopy. A mechanism of 2′,3′-anhydroinosine hydrolysis in D2O is fully determined for the first time.

Suicide Gene/Prodrug Therapy for Pancreatic Adenocarcinoma by E. Coli Purine Nucleoside Phosphorylase and 6-Methylpurine 2′-deoxyriboside

Pancreas ◽  
2004 ◽  
Vol 28 (2) ◽  
pp. e54-e64 ◽  
Author(s):  
Sophie Deharvengt ◽  
Séverine Wack ◽  
Muriel Uhring ◽  
Marc Aprahamian ◽  
Amor Hajri
Keyword(s):  
Pancreatic Adenocarcinoma ◽  
Purine Nucleoside Phosphorylase ◽  
Suicide Gene ◽  
Purine Nucleoside ◽  
Nucleoside Phosphorylase ◽  
E Coli
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