scholarly journals Antiidiotypic antibody related to the 84 kD human sperm membrane protein

Cell Research ◽  
1990 ◽  
Vol 1 (2) ◽  
pp. 163-172
Author(s):  
Jun Yu ◽  
Linfang Wang ◽  
Shiying Miao ◽  
Yuanchang Yan
Keyword(s):  
Membrane Protein ◽  
Human Sperm ◽  
Antiidiotypic Antibody ◽  
Sperm Membrane

scholarly journals Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by anti-human sperm membrane protein 1 antibody

2007 ◽  
Vol 9 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Guo-Yan Cheng ◽  
Jian-Li Shi ◽  
Min Wang ◽  
Yan-Qin Hu ◽  
Chun-Meng Liu ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Zona Pellucida ◽  
Acrosome Reaction ◽  
Human Sperm ◽  
Sperm Membrane

Expression and characterization of a novel human sperm membrane protein

1996 ◽  
Vol 31 (3) ◽  
pp. 230
Author(s):  
Q.-Y. Liu ◽  
Wang Lin Fang ◽  
Miao Shi Yang ◽  
J.F. Catterall
Keyword(s):  
Membrane Protein ◽  
Human Sperm ◽  
Sperm Membrane

scholarly journals Characterization of cDNA encoding a human sperm membrane protein related to A4 amyloid protein.

1990 ◽  
Vol 87 (7) ◽  
pp. 2405-2408 ◽  
Author(s):  
Y. C. Yan ◽  
Y. Bai ◽  
L. F. Wang ◽  
S. Y. Miao ◽  
S. S. Koide
Keyword(s):  
Membrane Protein ◽  
Human Sperm ◽  
Amyloid Protein ◽  
Sperm Membrane

Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody

1990 ◽  
Vol 10 (2) ◽  
pp. 131-139
Author(s):  
Oyewole Adeyemo ◽  
E. O. Okegbile ◽  
O. O. Olorunsogo
Keyword(s):  
Molecular Weight ◽  
Membrane Protein ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Partial Purification ◽  
Boar Sperm ◽  
Sperm Membrane ◽  
Triton X

For the development of immunological contraception, attention is being concentrated on the possibility of using a sperm membrane antigen. Boar sperm membrane was extracted with triton-X 100 and fractionated by Sephadex G-150 column chromatography. The glycosylated and nonglycosylated portions of protein peaks from the gel filtration were obtained by fractionating on concanavalin A-Sepharose and eluting the bound protein with 0.3 M methyl mannoside. A glycosylated fraction was found to induce sperm agglutinating antibodies in rabbit. The partially purified protein has a molecular weight of 30 kilodaltons, as determined by sodium dodecyl polyaccyrlamide gel electrophoresis. Further work is planned on the histochemical determination of the origin of this protein and species cross-activity of the antibody.

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