Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA

Abstract Background Brassica napus is the third leading source of edible oil in the world. Genic male sterility (GMS) lines provide crucial material for harnessing heterosis for rapeseed. GMS lines have been used successfully for rapeseed hybrid production in China. MicroRNAs (miRNAs) play crucial regulatory roles in various plant growth, development, and stress response processes. However, reports on miRNAs that regulate the pollen development of GMS lines in B. napus are few. Results In this study, 12 small RNA and transcriptome libraries were constructed and sequenced for the flower buds from the fertile and sterile lines of two recessive GMS (RGMS) lines, namely, “6251AB” and “6284AB”. At the same time, 12 small RNA and transcriptome libraries were also constructed and sequenced for the flower buds from the fertile and sterile lines of two dominant GMS (DGMS) lines, namely, “4001AB” and “4006AB”. Based on the results, 46 known miRNAs, 27 novel miRNAs on the other arm of known pre-miRNAs, and 44 new conserved miRNAs were identified. Thirty-five pairs of novel miRNA-3p/miRNA-5p were found. Among all the identified miRNAs, fifteen differentially expressed miRNAs with over 1.5-fold change between flower buds of sterile and fertile lines were identified, including six differentially expressed miRNAs between “4001A” and “4001B”, two differentially expressed miRNAs between “4006A” and “4006B”, four differentially expressed miRNAs between “6251A” and “6251B”, and ten differentially expressed miRNAs between “6284A” and “6284B”. The correlation analysis of small RNA and transcriptome sequencing was conducted. And 257 candidate target genes were predicted for the 15 differentially expressed miRNAs. The results of 5′ modified RACE indicated that BnaA09g48720D, BnaA09g11120D, and BnaCnng51960D were cleaved by bna-miR398a-3p, bna-miR158-3p and bna-miR159a, respectively. Among the differentially expressed miRNAs, miR159 was chosen to analyze its function. Overexpression of bna-miR159 in Arabidopsis resulted in decreased seed setting rate, and shortened siliques, illustrating that miR159 may regulate the fertility and silique development in rapeseed. Conclusions Our findings provide an overview of miRNAs that are potentially involved in GMS and pollen development. New information on miRNAs and their related target genes are provided to exploit the GMS mechanism and reveal the miRNA networks in B. napus.

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Identification of miRNAs And Their Target Genes In Genic Male Sterility Lines In Brassica Napus By Small RNA Sequencing

10.21203/rs.3.rs-471166/v1 ◽

2021 ◽

Author(s):

Jianxia Jiang ◽

Pengfei Xu ◽

Yajie Li ◽

Yanli Li ◽

Xirong Zhou ◽

...

Keyword(s):

Brassica Napus ◽

Male Sterility ◽

Small Rna ◽

Pollen Development ◽

Target Genes ◽

Differentially Expressed ◽

Small Rna Sequencing ◽

Flower Buds ◽

Genic Male Sterility ◽

Differentially Expressed Mirnas

Abstract Background: Brassica napus is the third leading source of edible oil in the world. Genic male sterility (GMS) lines provide crucial material for harnessing heterosis for rapeseed. GMS lines have been used successfully for rapeseed hybrid production in China. MicroRNAs (miRNAs) play crucial regulatory roles in various plant growth, development, and stress response processes. However, reports on miRNAs that regulate the pollen development of GMS lines in B. napus are few.Results: In this study, 12 small RNA and transcriptome libraries were constructed and sequenced for the flower buds from the fertile and sterile lines of two recessive GMS (RGMS) lines, namely, “6251AB” and “6284AB”. At the same time, 12 small RNA and transcriptome libraries were also constructed and sequenced for the flower buds from the fertile and sterile lines of two dominant GMS (DGMS) lines, namely, “4001AB” and “4006AB”. Based on the results, 46 known miRNAs, 27 novel miRNAs on the other arm of known pre-miRNAs, and 44 new conserved miRNAs were identified. Thirty-five pairs of novel miRNA-3p/miRNA-5p were found. Among all the identified miRNAs, 21 differentially expressed miRNAs with over 1.5-fold change between flower buds of sterile and fertile lines were identified, including six differentially expressed miRNAs between “4001A” and “4001B”, two differentially expressed miRNAs between “4006A” and “4006B”, six differentially expressed miRNAs between “6251A” and “6251B”, and 14 differentially expressed miRNAs between “6284A” and “6284B”. The correlation analysis of small RNA and transcriptome sequencing was conducted. The analysis results indicated that 360 genes were predicted to be the candidate targets of 21 differentially expressed miRNAs. BnaA09g48720D, BnaA09g11120D, and BnaCnng51960D were demonstrated to be cleaved by bna-miR398a-3p, bna-miR158-3p and bna-miR159a, respectively. Among the differentially expressed miRNAs, miR159 was chosen to analyze its function in reproduction development and male sterility. Overexpression of bna-miR159 in Arabidopsis resulted in decreased seed setting rate, and shortened siliques, illustrating that miR159 may regulate the fertility and silique development in rapeseed.Conclusions: Our findings provide an overview of miRNAs that are potentially involved in GMS and pollen development. New information on miRNAs and their related target genes are provided to exploit the GMS mechanism and reveal the miRNA networks in B. napus.

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Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.)

The Plant Journal ◽

10.1111/j.1365-313x.2008.03405.x ◽

2008 ◽

Vol 54 (2) ◽

pp. 190-204 ◽

Cited By ~ 68

Author(s):

Mi-Ok Woo ◽

Tae-Ho Ham ◽

Hyeon-So Ji ◽

Min-Seon Choi ◽

Wenzhu Jiang ◽

...

Keyword(s):

Oryza Sativa ◽

Male Sterility ◽

Oryza Sativa L ◽

Genic Male Sterility

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Three AFLP markers tightly linked to the genic male sterility ms 3 gene in chili pepper (Capsicum annuum L.) and conversion to a CAPS marker

Euphytica ◽

10.1007/s10681-009-0107-1 ◽

2009 ◽

Vol 173 (1) ◽

pp. 55-61 ◽

Cited By ~ 25

Author(s):

Jundae Lee ◽

Jae Bok Yoon ◽

Jung-Heon Han ◽

Won Phil Lee ◽

Sang Hoon Kim ◽

...

Keyword(s):

Male Sterility ◽

Capsicum Annuum ◽

Chili Pepper ◽

Aflp Markers ◽

Caps Marker ◽

Genic Male Sterility ◽

Capsicum Annuum L

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Genic male sterility

Pollen Biotechnology for Crop Production and Improvement ◽

10.1017/cbo9780511525469.010 ◽

1997 ◽

pp. 183-198 ◽

Cited By ~ 16

Author(s):

V. K. Sawhney

Keyword(s):

Male Sterility ◽

Genic Male Sterility

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A Sterility Induction Trait in the Genic Male Sterility Wheat Line 4110S Induced by High Temperature and its Cytological Response

Crop Science ◽

10.2135/cropsci2017.12.0714 ◽

2018 ◽

Vol 58 (5) ◽

pp. 1866-1876 ◽

Cited By ~ 2

Author(s):

Xuetong Yang ◽

Xingxia Geng ◽

Zihan Liu ◽

Jiali Ye ◽

Mengfan Xu ◽

...

Keyword(s):

High Temperature ◽

Male Sterility ◽

Wheat Line ◽

Genic Male Sterility

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Identification and Verification of Genes Related to Pollen Development and Male Sterility Induced by High Temperature in Thermo-sensitive Genic Male Sterile Wheat Line YanZhan 4110S

10.21203/rs.3.rs-119009/v1 ◽

2020 ◽

Author(s):

Xuetong Yang ◽

Jiali Ye ◽

Fuqiang Niu ◽

Yi Feng ◽

Xiyue Song

Keyword(s):

Gene Silencing ◽

Male Sterility ◽

Mosaic Virus ◽

Pollen Development ◽

Bioinformatics Analysis ◽

Virus Induced Gene Silencing ◽

Wheat Line ◽

Pollen Abortion ◽

Hub Genes ◽

Genic Male Sterility

Abstract Background: Environment-sensitive genic male sterility is of vital importance to hybrid vigor in crop production and breeding, therefore, it is meaningful to identify and study the function of the genes related to pollen development and male sterility, which still not fully understanding currently. In this study, Yanzhan 4110S, a new thermo-sensitive genic male sterility (TGMS) wheat line, and its near isogenic line Yanzhan 4110 were carried out cytological features observation, bioinformatics analysis to investgate the abortion state and identified the genes involved in pollen development which have fertility regulation function. Barely stripe mosaic virus-induced gene silencing was used to verify the genes function.Results: Cytological analysis showed pollen abortion event of Yanzhan 4110S occur at the later uninucleate stage (Lun) under higher temperature induction (day/night temperatures of 22 °C/20 °C), when the anthers were collected and assessed for transcriptomic profiling through high-throughput sequencing. We then in-depth analyzed the differentially expressed genes (DEGs) by Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, the results showed that the occurrence of Yanzhan 4110S male-sterility most likely related to metabolic pathway, including phenylpropanoid biosynthesis in the biosynthesis of other secondary metabolites, starch and sucrose metabolism in carbohydrate metabolism, carbon fixation in photosynthetic organisms as well as carbon metabolism in energy metabolism. The weighted gene co-expression network analysis in the transcriptome profiles further identified some hub genes, where the key genes involved in those pathways were intersection between the unique DEGs of Yanzhan 4110S in anther and hub genes, totally 228 genes, which were highly related to pollen development including TaMut11 and TaSF3. Moreover, further verification through barely stripe mosaic virus-induced gene silencing elucidated that the silencing of TaMut11 and TaSF3 caused pollen abortion, finally resulting in the declination of fertility. So, the genes TaMut11 and TaSF3 are related to fertility conversion of Yanzhan 4110S.Conclusion: Through comparative transcriptome bioinformatics analysis, the genes TaMut11 and TaSF3 associated with pollen development and male sterility induced by high temperature were identified in Yanzhan 4110S, and verificated by barely stripe mosaic virus-induced gene silencing. These findings provided researching the abortive mechanism in environment-sensitive genic male sterility wheat.

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