scholarly journals Rad51 Promoter-Targeted Gene Therapy Is Effective for In Vivo Visualization and Treatment of Cancer

2012 ◽  
Vol 20 (2) ◽  
pp. 347-355 ◽  
Author(s):  
Christopher M Hine ◽  
Andrei Seluanov ◽  
Vera Gorbunova
Keyword(s):  
Gene Therapy ◽  
Targeted Gene Therapy ◽  
Rad51 Promoter

scholarly journals Adenovirus-mediated hypoxia-targeted gene therapy using HSV thymidine kinase and bacterial nitroreductase prodrug-activating genes in vitro and in vivo

2011 ◽  
Vol 18 (11) ◽  
pp. 773-784 ◽  
Author(s):  
T J Harvey ◽  
I M Hennig ◽  
S D Shnyder ◽  
P A Cooper ◽  
N Ingram ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Thymidine Kinase ◽  
Targeted Gene Therapy ◽  
Hsv Thymidine Kinase

scholarly journals Targeted gene therapy and in vivo bioluminescent imaging for monitoring postsurgical recurrence and metastasis in mouse models of liver cancer

2016 ◽  
Vol 15 (3) ◽  
Author(s):  
Q. He ◽  
C.L. Yao ◽  
L. Li ◽  
Z. Xin ◽  
Z.K. Jing ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Liver Cancer ◽  
Mouse Models ◽  
Bioluminescent Imaging ◽  
Targeted Gene Therapy ◽  
Postsurgical Recurrence

scholarly journals Restoring the natural tropism of AAV2 vectors for human liver

2020 ◽  
Vol 12 (560) ◽  
pp. eaba3312
Author(s):  
Marti Cabanes-Creus ◽  
Claus V. Hallwirth ◽  
Adrian Westhaus ◽  
Boaz H. Ng ◽  
Sophia H.Y. Liao ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Tissue Culture ◽  
Human Liver ◽  
Xenograft Model ◽  
Human Hepatocytes ◽  
Targeted Gene Therapy ◽  
Tissue Culture Propagation ◽  
Made In

Recent clinical successes in gene therapy applications have intensified interest in using adeno-associated viruses (AAVs) as vectors for therapeutic gene delivery. Although prototypical AAV2 shows robust in vitro transduction of human hepatocyte–derived cell lines, it has not translated into an effective vector for liver-directed gene therapy in vivo. This is consistent with observations made in Fah−/−/Rag2−/−/Il2rg−/− (FRG) mice with humanized livers, showing that AAV2 functions poorly in this xenograft model. Here, we derived naturally hepatotropic AAV capsid sequences from primary human liver samples. We demonstrated that capsid mutations, likely acquired as an unintentional consequence of tissue culture propagation, attenuated the intrinsic human hepatic tropism of natural AAV2 and related human liver AAV isolates. These mutations resulted in amino acid changes that increased binding to heparan sulfate proteoglycan (HSPG), which has been regarded as the primary cellular receptor mediating AAV2 infection of human hepatocytes. Propagation of natural AAV variants in vitro showed tissue culture adaptation with resulting loss of tropism for human hepatocytes. In vivo readaptation of the prototypical AAV2 in FRG mice with a humanized liver resulted in restoration of the intrinsic hepatic tropism of AAV2 through decreased binding to HSPG. Our results challenge the notion that high affinity for HSPG is essential for AAV2 entry into human hepatocytes and suggest that natural AAV capsids of human liver origin are likely to be more effective for liver-targeted gene therapy applications than culture-adapted AAV2.

Abstract 2: Naked CanScript, an 18-base pair sequence, has tumor cell-specific promoter activity in vivo: Implications for targeted gene therapy

2010 ◽  
Author(s):  
Janet A. Sawicki ◽  
Yu-Hung Huang ◽  
Joseph Cozzitorto ◽  
Robert Langer ◽  
Daniel G. Anderson ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Tumor Cell ◽  
Base Pair ◽  
Promoter Activity ◽  
Pair Sequence ◽  
Targeted Gene Therapy

scholarly journals A Microsphere-Liposome (Microplex) Vector for Targeted Gene Therapy of Cancer. II. In Vivo Biodistribution Study in a Solid Tumor Model

Drug Delivery ◽  
2000 ◽  
Vol 7 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Crispin R. Dass ◽  
Todd L. Walker ◽  
Wouter H. J. Kalle ◽  
Mark A. Burton
Keyword(s):  
Gene Therapy ◽  
Solid Tumor ◽  
Tumor Model ◽  
Biodistribution Study ◽  
In Vivo Biodistribution ◽  
Targeted Gene Therapy ◽  
Solid Tumor Model

scholarly journals Regeneration of pancreatic islets in vivo by ultrasound-targeted gene therapy

Gene Therapy ◽  
2010 ◽  
Vol 17 (11) ◽  
pp. 1411-1420 ◽  
Author(s):  
S Chen ◽  
M Shimoda ◽  
M-Y Wang ◽  
J Ding ◽  
H Noguchi ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Pancreatic Islets ◽  
Targeted Gene Therapy

In vivo laser targeted gene therapy of retina ganglion cells (Conference Presentation)

2019 ◽  
Author(s):  
Ariel Wilson ◽  
Javier Mazzaferri ◽  
Éric Bergeron ◽  
Sergiy Patskovsky ◽  
Paule Marcoux-Valiquette ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Ganglion Cells ◽  
Targeted Gene Therapy

Targeted gene therapy

2016 ◽  
Author(s):  
Angelo Lombardo
Keyword(s):  
Gene Therapy ◽  
Targeted Gene Therapy
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