Enhancement of Gene Expression by Somatic Hybridization with Primary Cells: High-Level Synthesis of the Hepatitis B Surface Antigen in Monkey Vero Cells by Fusion with Primary Hepatocytes

1990 ◽  
Vol 8 (9) ◽  
pp. 858-862 ◽  
Author(s):  
Nicole Chenciner ◽  
Francis Delpeyroux ◽  
Nicole Israel ◽  
Mireille Lambert ◽  
Annick Lim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Somatic Hybridization ◽  
Hepatitis B Surface Antigen ◽  
Vero Cells ◽  
High Level Synthesis ◽  
Primary Cells ◽  
Primary Hepatocytes ◽  
High Level

scholarly journals Hepatitis B surface antigen gene expression is regulated by sex steroids and glucocorticoids in transgenic mice.

1987 ◽  
Vol 84 (5) ◽  
pp. 1187-1191 ◽  
Author(s):  
H. Farza ◽  
A. M. Salmon ◽  
M. Hadchouel ◽  
J. L. Moreau ◽  
C. Babinet ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Sex Steroids ◽  
Hepatitis B Surface Antigen ◽  
Antigen Gene

Effect of an extract from Phyllanthus amarus on hepatitis B surface antigen gene expression in human hepatoma cells

1993 ◽  
Vol 20 (3) ◽  
pp. 185-192 ◽  
Author(s):  
Sheau-Farn Yeh ◽  
Chuang-Ye Hong ◽  
Yu-Lun Huang ◽  
Tsung-Yun Liu ◽  
Kong-Bung Choo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Hepatoma Cells ◽  
Phyllanthus Amarus ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Antigen Gene

scholarly journals False-Negative Hepatitis B Virus (HBV) Surface Antigen in a Vaccinated Dialysis Patient with a High Level of HBV DNA in the United States

2012 ◽  
Vol 19 (5) ◽  
pp. 820-822 ◽  
Author(s):  
Matthew C. Foy ◽  
Chloe L. Thio ◽  
Hyon S. Hwang ◽  
Melissa Saulynas ◽  
James P. Hamilton ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hemodialysis Patient ◽  
Hepatitis B Surface Antigen ◽  
False Negative ◽  
The United States ◽  
Hbv Dna ◽  
B Virus ◽  
High Level

ABSTRACTScreening with hepatitis B surface antigen (HBsAg) is highly recommended for at-risk individuals. Mutations in the HBsAg can result in an inability to detect the virus during routine screening. We describe a hemodialysis patient found to have high levels of hepatitis B virus (HBV) DNA and HBV antibody but negative HBsAg on two routine assays.

Differential gene expression, irrespective of circulating Hepatitis B Surface Antigen levels, between Inactive Carrier and Nucleos(t)ide Analogue-Treated Hepatitis B Virus patients

2020 ◽  
Author(s):  
Noé R Montanari ◽  
Nádia Conceição-Neto ◽  
Ilse Van Den Wyngaert ◽  
Gertine W Van Oord ◽  
Zwier M A Groothuismink ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Inactive Carrier ◽  
B Virus ◽  
Infected Liver ◽  
Differential Gene ◽  
Chronic Hbv

Abstract Long-term viremia control in chronic HBV patients occurs either spontaneously in inactive carrier (IC) patients or therapy-induced by nucleos(t)ide analogues (NUC). To better understand the characteristics of viremia control, we evaluated gene expression in purified leukocyte subsets from IC versus NUC-treated patients, and evaluated the putative modulatory effects of hepatitis B surface antigen (HBsAg). We observed that gene expression in NUC-treated patients differed markedly from IC patients, especially in dendritic cells, monocytes, and CD8+ T cells, while serum HBsAg levels had little effect. Nevertheless, based on our findings it cannot be excluded that HBsAg may act locally in the infected liver or preferentially affects HBV-specific cells.

Maternal inhibition of hepatitis B surface antigen gene expression in transgenic mice correlates with de novo methylation

Nature ◽  
1987 ◽  
Vol 329 (6138) ◽  
pp. 454-456 ◽  
Author(s):  
Michelle Hadchouel ◽  
Hend Farza ◽  
Dominique Simon ◽  
Pierre Tiollais ◽  
Christine Pourcel
Keyword(s):  
Gene Expression ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
De Novo ◽  
Hepatitis B Surface Antigen ◽  
Antigen Gene ◽  
De Novo Methylation

scholarly journals HD-03/ES: A Herbal Medicine Inhibits Hepatitis B Surface Antigen Secretion in Transfected Human Hepatocarcinoma PLC/PRF/5 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-6
Author(s):  
Sandeep R. Varma ◽  
R. Sundaram ◽  
S. Gopumadhavan ◽  
Satyakumar Vidyashankar ◽  
Pralhad S. Patki
Keyword(s):  
Gene Expression ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Enzyme Assay ◽  
Culture Media ◽  
Hepatitis B Surface Antigen ◽  
Specific Primers ◽  
Its Gene ◽  
Acute And Chronic Hepatitis

HD-03/ES is a herbal formulation used for the treatment of hepatitis B. However, the molecular mechanism involved in the antihepatitis B (HBV) activity of this drug has not been studied using in vitro models. The effect of HD-03/ES on hepatitis B surface antigen (HBsAg) secretion and its gene expression was studied in transfected human hepatocarcinoma PLC/PRF/5 cells. The anti-HBV activity was tested based on the inhibition of HBsAg secretion into the culture media, as detected by HBsAg-specific antibody-mediated enzyme assay (ELISA) at concentrations ranging from 125 to 1000 μg/mL. The effect of HD-03/ES on HBsAg gene expression was analyzed using semiquantitative multiplex RT-PCR by employing specific primers. The results showed that HD-03/ES suppressed HBsAg production with an IC50 of 380 μg/mL in PLC/PRF/5 cells for a period of 24 h. HD-03/ES downregulated HBsAg gene expression in PLC/PRF/5 cells. In conclusion, HD-03/ES exhibits strong anti-HBV properties by inhibiting the secretion of hepatitis B surface antigen in PLC/PRF/5 cells, and this action is targeted at the transcription level. Thus, HD-03/ES could be beneficial in the treatment of acute and chronic hepatitis B infections.

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