scholarly journals A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Patrick M. Shih ◽  
Khanh Vuu ◽  
Nasim Mansoori ◽  
Leïla Ayad ◽  
Katherine B. Louie ◽  
...  
Keyword(s):  
Synthetic Biology ◽  
Gene Stacking ◽  
Plant Synthetic Biology

GondenBraid2.0: A comprehensive toolkit for plant synthetic biology

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
A Sarrion-Perdigones ◽  
M Vazquez-Vilar ◽  
J Palaci ◽  
A Granell ◽  
D Orzáez
Keyword(s):  
Synthetic Biology ◽  
Plant Synthetic Biology

Enabling plant synthetic biology through genome engineering

2015 ◽  
Vol 33 (2) ◽  
pp. 120-131 ◽  
Author(s):  
Nicholas J. Baltes ◽  
Daniel F. Voytas
Keyword(s):  
Synthetic Biology ◽  
Genome Engineering ◽  
Plant Synthetic Biology

scholarly journals The Multiplanetary Future of Plant Synthetic Biology

Genes ◽  
2018 ◽  
Vol 9 (7) ◽  
pp. 348 ◽  
Author(s):  
Briardo Llorente ◽  
Thomas Williams ◽  
Hugh Goold
Keyword(s):  
Synthetic Biology ◽  
Plant Synthetic Biology

scholarly journals Streamlined Construction of the Cyanobacterial CO2-Fixing Organelle via Protein Domain Fusions for Use in Plant Synthetic Biology

2015 ◽  
Vol 27 (9) ◽  
pp. 2637-2644 ◽  
Author(s):  
C. Raul Gonzalez-Esquer ◽  
Tyler B. Shubitowski ◽  
Cheryl A. Kerfeld
Keyword(s):  
Synthetic Biology ◽  
Protein Domain ◽  
Plant Synthetic Biology

scholarly journals Standards for plant synthetic biology: a common syntax for exchange ofDNAparts

2015 ◽  
Vol 208 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Nicola J. Patron ◽  
Diego Orzaez ◽  
Sylvestre Marillonnet ◽  
Heribert Warzecha ◽  
Colette Matthewman ◽  
...  
Keyword(s):  
Synthetic Biology ◽  
Plant Synthetic Biology

scholarly journals A reversible memory switch for plant synthetic biology based on the phage PhiC31 integration system

2019 ◽  
Author(s):  
Bernabé-Orts Joan Miquel ◽  
Quijano-Rubio Alfredo ◽  
Mancheño-Bonillo Javier ◽  
Moles-Casas Victor ◽  
Selma Sara ◽  
...  
Keyword(s):  
Synthetic Biology ◽  
Cell Fate ◽  
Regulatory Element ◽  
Living Organism ◽  
Inducible Promoter ◽  
Chemical Stimulus ◽  
Toggle Switch ◽  
Genetic Switch ◽  
Whole Plants ◽  
Plant Synthetic Biology

ABSTRACTPlant synthetic biology aims to contribute to global food security by engineering plants with new or improved functionalities. In recent years, synthetic biology has rapidly advanced from the setup of basic genetic devices to the design of increasingly complex gene circuits able to provide organisms with novel functions. While many bacterial, fungal and mammalian unicellular chassis have been extensively engineered, this progress has been delayed in plants due to their complex multicellular nature and the lack of reliable DNA devices that allow an accurate design of more sophisticated biological circuits. Among these basic devices, gene switches are crucial to deploying new layers of regulation into the engineered organisms. Of special interest are bistable genetic toggle switches, which allow a living organism to exist in two alternative states and switch between them with a minimal metabolic burden. Naturally occurring toggle switches control important decision-making processes such as cell fate and developmental events. We sought to engineer whole plants with an orthogonal genetic toggle switch to be able to regulate artificial functions with minimal interference with their natural pathways. Here we report a bistable toggle memory switch for whole plants based on the phage PhiC31 serine integrase and its cognate recombination directionality factor (RDF). This genetic device was designed to control the transcription of two genes of interest by inversion of a central DNA regulatory element. Each state of the device is defined by one transcriptionally active gene of interest, while the other one remains inactive. The state of the switch can be reversibly modified by the action of the recombination actuators, which were administered externally (e.g. via agroinfiltration), or produced internally in response to an inducible chemical stimulus. We extensively characterized the kinetics, memory, and reversibility of this genetic switch in Nicotiana benthamiana through transient and stable transformation experiments using transgenic plants and hairy roots. Finally, we coupled the integrase expression to an estradiol-inducible promoter as a proof of principle of inducible activation of the switch.

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