Ribosomal RNA Synthesis in E. coli

doi:10.1038/newbio234097a0

Temperature-sensitive mutation. V. Infection of ts-mutant of RNA phage R17 and host cell metabolism

Canadian Journal of Biochemistry ◽

10.1139/o70-009 ◽

1970 ◽

Vol 48 (1) ◽

pp. 47-54 ◽

Cited By ~ 1

Author(s):

S. J. Igarashi ◽

J. F. Elliott ◽

R. P. Bissonnette

Keyword(s):

Host Cell ◽

Ribosomal Rna ◽

Rna Synthesis ◽

Cell Metabolism ◽

Temperature Sensitive ◽

Specific Inhibition ◽

Sensitive Mutant ◽

E Coli ◽

Ts Mutant ◽

Rna Phage

A temperature-sensitive mutant of RNA bacteriophage R17, ts24, which contains a ts-mutation in the RNA-replicating function, was used to analyze the effects of RNA phage infection on host E. coli cell metabolism in the presence and absence of phage RNA synthesis. The principal conclusions from these studies are as follows. (1) Host ribosomal RNA synthesis is inhibited by infection with R17 whether or not phage-specific RNA synthesis takes place. (2) Immediately following infection, overall protein synthesis is reduced to 10–20% of the level of synthesis occurring in noninfected cultures. (3) Phage RNA remains intact in the host cell for at least 90 min after infection. Based on these observations, a mechanism for the specific inhibition of ribosomal RNA by R17 infection was discussed.

Regulation of Ribosomal RNA Synthesis and Control of Ribosome Formation in E. Coli

The Translational Apparatus ◽

10.1007/978-1-4615-2407-6_12 ◽

1993 ◽

pp. 119-130 ◽

Cited By ~ 1

Author(s):

Rolf Wagner ◽

Günter Theißen ◽

Martin Zacharias

Keyword(s):

Ribosomal Rna ◽

Rna Synthesis ◽

E Coli ◽

And Control ◽

Ribosome Formation

Specific stimulation of ribosomal RNA synthesis in E. coli by a protein factor

MGG Molecular & General Genetics ◽

10.1007/bf00267441 ◽

1980 ◽

Vol 177 (2) ◽

pp. 291-295 ◽

Cited By ~ 9

Author(s):

Ben A. Oostra ◽

AB Geert ◽

Max Gruber

Keyword(s):

Ribosomal Rna ◽

Rna Synthesis ◽

E Coli ◽

Protein Factor ◽

Specific Stimulation ◽

Stimulation Of

Tandem promoters direct E. coli ribosomal RNA synthesis

Cell ◽

10.1016/0092-8674(79)90310-6 ◽

1979 ◽

Vol 17 (1) ◽

pp. 225-234 ◽

Cited By ~ 70

Author(s):

Richard A. Young ◽

Joan A. Steitz

Keyword(s):

Ribosomal Rna ◽

Rna Synthesis ◽

E Coli

Visualisation of E. coli ribosomal RNA in situ by electron spectroscopic imaging and image averaging

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100122733 ◽

1992 ◽

Vol 50 (1) ◽

pp. 466-467

Author(s):

Daniel Beniac ◽

George Harauz

Keyword(s):

Ribosomal Rna ◽

Three Dimensional ◽

Spectroscopic Imaging ◽

Electron Spectroscopic Imaging ◽

E Coli ◽

Microscopical Technique ◽

Quantitative Image ◽

Dimensional Reconstruction ◽

Image Averaging ◽

Protein Components

The structures of E. coli ribosomes have been extensively probed by electron microscopy of negatively stained and frozen hydrated preparations. Coupled with quantitative image analysis and three dimensional reconstruction, such approaches are worthwhile in defining size, shape, and quaternary organisation. The important question of how the nucleic acid and protein components are arranged with respect to each other remains difficult to answer, however. A microscopical technique that has been proposed to answer this query is electron spectroscopic imaging (ESI), in which scattered electrons with energy losses characteristic of inner shell ionisations are used to form specific elemental maps. Here, we report the use of image sorting and averaging techniques to determine the extent to which a phosphorus map of isolated ribosomal subunits can define the ribosomal RNA (rRNA) distribution within them.

Faculty Opinions recommendation of Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1024764.293740 ◽

2005 ◽

Author(s):

Lucio Comai

Keyword(s):

Ribosomal Rna ◽

Rna Synthesis ◽

Drosophila Development

Faculty Opinions recommendation of A Molecular Titration System Coordinates Ribosomal Protein Gene Transcription with Ribosomal RNA Synthesis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726937139.793528937 ◽

2017 ◽

Author(s):

Catherine Fox

Keyword(s):

Ribosomal Protein ◽

Gene Transcription ◽

Ribosomal Rna ◽

Rna Synthesis ◽

Protein Gene ◽

Ribosomal Protein Gene ◽

Titration System ◽

Molecular Titration

Detection of residual E. coli host cell DNA by 23S ribosomal RNA gene-targeted quantitative polymerase chain reactions

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2021.114000 ◽

2021 ◽

Vol 198 ◽

pp. 114000

Author(s):

Dehua Li ◽

Qian Zhang ◽

Guodi Liu ◽

Linsong Zhang ◽

Zhangjie Gu ◽

...

Keyword(s):

Host Cell ◽

Ribosomal Rna ◽

Ribosomal Rna Gene ◽

Chain Reactions ◽

E Coli ◽

Polymerase Chain Reactions ◽

Polymerase Chain ◽

23S Ribosomal Rna

Accelerated rates of ribosomal RNA synthesis during growth of contracting heart cells in culture

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)84700-2 ◽

1989 ◽

Vol 264 (30) ◽

pp. 18220-18227

Author(s):

P J McDermott ◽

L I Rothblum ◽

S D Smith ◽

H E Morgan

Keyword(s):

Ribosomal Rna ◽

Rna Synthesis ◽

Heart Cells ◽

Cells In Culture ◽

Contracting Heart

DIFFERENTIATION OF DROSOPHILA CELLS LACKING RIBOSOMAL DNA, IN VITRO

Genetics ◽

10.1093/genetics/73.3.429 ◽

1973 ◽

Vol 73 (3) ◽

pp. 429-434

Author(s):

J James Donady ◽

R L Seecof ◽

M A Fox

Keyword(s):

Drosophila Melanogaster ◽

Ribosomal Rna ◽

Ribosomal Dna ◽

Rna Synthesis ◽

Wild Type ◽

Drosophila Cells

ABSTRACT Drosophila melanogaster embryos that lacked ribosomal DNA were obtained from appropriate crosses. Cells were taken from such embryos before overt differentiation took place and were cultured in vitro. These cells differentiated into neurons and myocytes with the same success as did wild-type controls. Therefore, ribosomal RNA synthesis is not necessary for the differentiation of neurons and myocytes in vitro.