Specific stimulation of ribosomal RNA synthesis in E. coli by a protein factor

1980 ◽  
Vol 177 (2) ◽  
pp. 291-295 ◽  
Author(s):  
Ben A. Oostra ◽  
AB Geert ◽  
Max Gruber
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
E Coli ◽  
Protein Factor ◽  
Specific Stimulation ◽  
Stimulation Of

scholarly journals Ornithine decarboxylase and ribosomal RNA synthesis during the stimulation of lymphocytes by phytohaemagglutinin

FEBS Letters ◽  
1971 ◽  
Vol 16 (1) ◽  
pp. 9-12 ◽  
Author(s):  
John E. Kay ◽  
Anne Cooke
Keyword(s):  
Ornithine Decarboxylase ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Stimulation Of

scholarly journals Ribosomal RNA Synthesis in E. coli

1971 ◽  
Vol 234 (47) ◽  
pp. 97-97 ◽  
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
E Coli

Temperature-sensitive mutation. V. Infection of ts-mutant of RNA phage R17 and host cell metabolism

1970 ◽  
Vol 48 (1) ◽  
pp. 47-54 ◽  
Author(s):  
S. J. Igarashi ◽  
J. F. Elliott ◽  
R. P. Bissonnette
Keyword(s):  
Host Cell ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Cell Metabolism ◽  
Temperature Sensitive ◽  
Specific Inhibition ◽  
Sensitive Mutant ◽  
E Coli ◽  
Ts Mutant ◽  
Rna Phage

A temperature-sensitive mutant of RNA bacteriophage R17, ts24, which contains a ts-mutation in the RNA-replicating function, was used to analyze the effects of RNA phage infection on host E. coli cell metabolism in the presence and absence of phage RNA synthesis. The principal conclusions from these studies are as follows. (1) Host ribosomal RNA synthesis is inhibited by infection with R17 whether or not phage-specific RNA synthesis takes place. (2) Immediately following infection, overall protein synthesis is reduced to 10–20% of the level of synthesis occurring in noninfected cultures. (3) Phage RNA remains intact in the host cell for at least 90 min after infection. Based on these observations, a mechanism for the specific inhibition of ribosomal RNA by R17 infection was discussed.

scholarly journals The cyclo-oxygenase inhibitors indomethacin and ibuprofen inhibit the insulin-induced stimulation of ribosomal RNA synthesis in L6 myoblasts

1989 ◽  
Vol 264 (1) ◽  
pp. 101-106 ◽  
Author(s):  
R M Palmer ◽  
G P Campbell ◽  
P F Whitelaw ◽  
D S Brown ◽  
P A Bain ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Acid Metabolism ◽  
Arachidonic Acid Metabolism ◽  
High Concentration ◽  
Ribosomal Subunits ◽  
Rrna Species ◽  
Inflammatory Drugs ◽  
Stimulation Of

Insulin stimulated total RNA accretion and the incorporation of [3H]uridine into RNA in L6 skeletal-muscle myoblasts. Incorporation of uridine into the rRNA was measured after either separation of 18 S and 28 S rRNA species by agarose-gel electrophoresis or separation of dissociated 40 S and 60 S ribosomal subunits on sucrose density gradients. Both methods showed a stimulation by insulin of uridine incorporation into the RNA of the two subunits. Two non-steroidal anti-inflammatory drugs, indomethacin and ibuprofen, which inhibit the metabolism of arachidonic acid by the cyclo-oxygenase pathway, inhibited the insulin-induced accretion of total cellular RNA and the incorporation of uridine into the RNA of both ribosomal subunits. The effect of insulin was observed both by using a tracer dose of [3H]uridine (5 microM) and in the presence of a high concentration (1 mM) of uridine to minimize possible changes in intracellular precursor pools. Neither insulin nor indomethacin was found to affect the incorporation of uridine into the total intracellular nucleotide pool, or the conversion of uridine into UTP. The ability of inhibitors of arachidonic acid metabolism to prevent insulin-induced increases in RNA metabolism suggests that a prostaglandin or other eicosanoid is involved in the signal mechanism whereby insulin stimulates RNA synthesis.

scholarly journals STIMULATION BY INSULIN OF RNA SYNTHESIS IN CHICK FIBROBLASTS

1974 ◽  
Vol 60 (1) ◽  
pp. 54-64 ◽  
Author(s):  
Joel B. Baseman ◽  
Domenic Paolini ◽  
Harold Amos
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Polymerase Activity ◽  
Serum Free Medium ◽  
5S Rna ◽  
Fresh Serum ◽  
Protein And Rna Synthesis ◽  
Rna Polymerase Activity ◽  
Kinetics Of ◽  
Stimulation Of

After the addition of insulin to monolayers of chick fibroblasts previously incubated in serum-free medium, the rates of protein and RNA synthesis increase continuously during the first 8–10 h. Little stimulation of DNA synthesis or mitosis results with the addition of insulin alone in contrast to the addition of fresh serum which stimulates both markedly. The stimulation in RNA synthesis does not result from expansion of the nucleotide pool but is correlated with increases in RNA polymerase activity. All major classes of RNA are stimulated; processing of preribosomal RNA to 28S and 18S and the association of this mature RNA with ribosomes appear to occur normally. The kinetics of stimulation of 5S RNA differ from those of the synthesis of 4S and of ribosomal RNA. Insulin and serum appear to affect the synthesis or stability of certain transcripts differentially.

Specific stimulation of RNA synthesis by methionine in several strains of Escherichia coli

1966 ◽  
Vol 21 (1) ◽  
pp. 13-27 ◽  
Author(s):  
Eliora Z. Ron ◽  
Bernard D. Davis
Keyword(s):  
Escherichia Coli ◽  
Rna Synthesis ◽  
Specific Stimulation ◽  
Stimulation Of

Regulation of Ribosomal RNA Synthesis and Control of Ribosome Formation in E. Coli

1993 ◽  
pp. 119-130 ◽  
Author(s):  
Rolf Wagner ◽  
Günter Theißen ◽  
Martin Zacharias
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
E Coli ◽  
And Control ◽  
Ribosome Formation
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