Simplified behavioural tests for transient social memory in male mice and dams

In this study we evaluated the time dependence in cadmium-nicotine interaction and its effect on motor function, anxiety linked behavioural changes, serum electrolytes, and weight after acute and chronic treatment in adult male mice. Animals were separated randomly into four groups of n = 6 animals each. Treatment was done with nicotine, cadmium, or nicotine-cadmium for 21 days. A fourth group received normal saline for the same duration (control). Average weight was determined at 7-day interval for the acute (D1-D7) and chronic (D7-D21) treatment phases. Similarly, the behavioural tests for exploratory motor function (open field test) and anxiety were evaluated. Serum electrolytes were measured after the chronic phase. Nicotine, cadmium, and nicotine-cadmium treatments caused no significant change in body weight after the acute phase while cadmium-nicotine and cadmium caused a decline in weight after the chronic phase. This suggests the role of cadmium in the weight loss observed in tobacco smoke users. Both nicotine and cadmium raised serum Ca2+ concentration and had no significant effect on K+ ion when compared with the control. In addition, nicotine-cadmium treatment increased bioaccumulation of Cd2+ in the serum which corresponded to a decrease in body weight, motor function, and an increase in anxiety.

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SK3-overexpression drives sex-dimorphic working and social memory impairment in mice

10.1101/2020.07.31.230656 ◽

2020 ◽

Author(s):

Alline C Campos ◽

Franciele F Scarante ◽

Sabine Martin ◽

Marcio Lazzarini ◽

Doris Hermes ◽

...

Keyword(s):

Social Memory ◽

Hippocampal Formation ◽

Memory Performance ◽

Hippocampal Neurogenesis ◽

Adult Hippocampal Neurogenesis ◽

Estrogen Signaling ◽

K Channel ◽

Male Mice ◽

Female Mice ◽

Type 3

AbstractAlthough sex differences in memory tasks dependent on hippocampal function have been described in several species, including rodents and humans, the exact mechanisms involved remain debatable. The function of the small-conductance Ca2+-activated K+ channel type 3 has been associated with cognitive deficits, and its overexpression in male mice (T/T) induces shrinkage of the hippocampus. Here we describe that opposite to the observation in males, in female mice, SK3-induced-reduction in the volume of the hippocampal formation does not interfere with working and social memory performance. Male, but not female T/T mice showed decreased adult hippocampal neurogenesis and down-regulation of the expression of the genes related to Akt/mTOR and MAP kinase pathways. T/T male mice exhibit impaired estrogen and Neurogulin 1 signaling. An increased number of filopodia spines is observed in the dentate gyrus (DG). Our results suggest a fine-tune modulation of SK3 expression participates in the sex-dependent function of the hippocampus via estrogen signaling and neuroplasticity in the DG. Our results reinforce the importance of testing male and female mice while conducting experiments with transgenic mice.

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Cholinergic transmission from the basal forebrain modulates social memory in male mice

European Journal of Neuroscience ◽

10.1111/ejn.15400 ◽

2021 ◽

Author(s):

Ornela Kljakic ◽

Mohammed Al‐Onaizi ◽

Helena Janíčková ◽

Kevin S. Chen ◽

Monica S. Guzman ◽

...

Keyword(s):

Basal Forebrain ◽

Social Memory ◽

Cholinergic Transmission ◽

Male Mice

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Neuropeptide Y prolongs non-social memory in a brain region- and receptor-specific way in male mice

Neuropharmacology ◽

10.1016/j.neuropharm.2020.108199 ◽

2020 ◽

Vol 175 ◽

pp. 108199

Author(s):

Johannes Kornhuber ◽

Iulia Zoicas

Keyword(s):

Neuropeptide Y ◽

Brain Region ◽

Social Memory ◽

Male Mice

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Neuropeptide Y prolongs non-social memory and differentially affects acquisition, consolidation, and retrieval of non-social and social memory in male mice

Scientific Reports ◽

10.1038/s41598-017-07273-x ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 7

Author(s):

Johannes Kornhuber ◽

Iulia Zoicas

Keyword(s):

Neuropeptide Y ◽

Social Memory ◽

Male Mice

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Ultrastructural Lesions Produced by Alcohol and Sucrose in the Heart and Liver of C57BL Mice

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068035 ◽

1970 ◽

Vol 28 ◽

pp. 208-209

Author(s):

K.K. SEKHRI ◽

C.S. ALEXANDER ◽

H.T. NAGASAWA

Keyword(s):

Osmium Tetroxide ◽

Male Mice ◽

Alcohol Group ◽

Group Iii ◽

Group I ◽

C57bl Mice ◽

Group Ii

C57BL male mice (Jackson Lab., Bar Harbor, Maine) weighing about 18 gms were randomly divided into three groups: group I was fed sweetened liquid alcohol diet (modified Schenkl) in which 36% of the calories were derived from alcohol; group II was maintained on a similar diet but alcohol was isocalorically substituted by sucrose; group III was fed regular mouse chow ad lib for five months. Liver and heart tissues were fixed in 2.5% cacodylate buffered glutaraldehyde, post-fixed in 2% osmium tetroxide and embedded in Epon-araldite.

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