Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells

2013 ◽  
Vol 20 (9) ◽  
pp. 1131-1139 ◽  
Author(s):  
Liying Yan ◽  
Mingyu Yang ◽  
Hongshan Guo ◽  
Lu Yang ◽  
Jun Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Embryonic Stem ◽  
Preimplantation Embryos ◽  
Rna Seq ◽  
Human Preimplantation Embryos

scholarly journals Pooled CRISPR-activation screening coupled with single-cell RNA-seq in mouse embryonic stem cells

2021 ◽  
Vol 2 (2) ◽  
pp. 100426
Author(s):  
Celia Alda-Catalinas ◽  
Melanie A. Eckersley-Maslin ◽  
Wolf Reik
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Rna Seq ◽  
Crispr Activation

scholarly journals Gene expression variability in mammalian embryonic stem cells using single cell RNA-seq data

2016 ◽  
Vol 63 ◽  
pp. 52-61 ◽  
Author(s):  
Anna Mantsoki ◽  
Guillaume Devailly ◽  
Anagha Joshi
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Embryonic Stem ◽  
Rna Seq ◽  
Expression Variability

scholarly journals Tracing the Derivation of Embryonic Stem Cells from the Inner Cell Mass by Single-Cell RNA-Seq Analysis

2010 ◽  
Vol 6 (5) ◽  
pp. 468-478 ◽  
Author(s):  
Fuchou Tang ◽  
Catalin Barbacioru ◽  
Siqin Bao ◽  
Caroline Lee ◽  
Ellen Nordman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Rna Seq

Self-Correction of Chromosomal Abnormalities in Human Preimplantation Embryos and Embryonic Stem Cells

2013 ◽  
Vol 22 (17) ◽  
pp. 2449-2456 ◽  
Author(s):  
Masood Bazrgar ◽  
Hamid Gourabi ◽  
Mojtaba Rezazadeh Valojerdi ◽  
Poopak Eftekhari Yazdi ◽  
Hossein Baharvand
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Chromosomal Abnormalities ◽  
Embryonic Stem ◽  
Preimplantation Embryos ◽  
Human Preimplantation Embryos

scholarly journals Identification of New Transcription Factors that Can Promote Pluripotent Reprogramming

2021 ◽  
Author(s):  
Ping Huang ◽  
Jieying Zhu ◽  
Yu Liu ◽  
Guihuan Liu ◽  
Ran Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Rna Sequencing ◽  
Human Urine ◽  
Embryonic Stem ◽  
Rna Seq ◽  
Reprogramming Efficiency ◽  
Yamanaka Factors ◽  
Urine Cells

Abstract Background Four transcription factors, Oct4, Sox2, Klf4, and c-Myc (the Yamanka factors), can reprogram somatic cells to induced pluripotent stem cells (iPSCs). Many studies have provided a number of alternative combinations to the non-Yamanaka factors. However, it is clear that many additional transcription factors that can generate iPSCs remain to be discovered. Methods The chromatin accessibility and transcriptional level of human embryonic stem cells and human urine cells were compared by Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) and RNA sequencing (RNA-seq) to identify potential reprogramming factors. Selected transcription factors were employed to reprogram urine cells, and the reprogramming efficiency was measured. Urine-derived iPSCs were detected for pluripotency by Immunofluorescence, quantitative polymerase chain reaction, RNA sequencing and teratoma formation test. Finally, we assessed the differentiation potential of the new iPSCs to cardiomyocytes in vitro. Results ATAC-seq and RNA-seq datasets predicted TEAD2, TEAD4 and ZIC3 as potential factors involved in urine cell reprogramming. Transfection of TEAD2, TEAD4 and ZIC3 (in the presence of Yamanaka factors) significantly improved the reprogramming efficiency of urine cells. We confirmed that the newly generated iPSCs possessed pluripotency characteristics similar to normal H1 embryonic stem cells. We also confirmed that the new iPSCs could differentiate to functional cardiomyocytes. Conclusions In conclusion, TEAD2, TEAD4 and ZIC3 can increase the efficiency of reprogramming human urine cells into iPSCs, and provides a new stem cell sources for the clinical application and modeling of cardiovascular disease. Graphical abstract

Expression of mRNAs for DNA methyltransferases and methyl-CpG-binding proteins in the human female germ line, preimplantation embryos, and embryonic stem cells

2004 ◽  
Vol 67 (3) ◽  
pp. 323-336 ◽  
Author(s):  
J. Huntriss ◽  
M. Hinkins ◽  
B. Oliver ◽  
S.E. Harris ◽  
J.C. Beazley ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Binding Proteins ◽  
Germ Line ◽  
Embryonic Stem ◽  
Dna Methyltransferases ◽  
Preimplantation Embryos ◽  
Human Female ◽  
Female Germ Line
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