Expression and purification of recombinant human L-OPA1 using BmNPV bacmid-silkworm expression system

2017 ◽  
Author(s):  
Tadato Ban ◽  
Tadato Ban ◽  
Naotada Ishihara
Keyword(s):  
Expression System ◽  
Expression And Purification ◽  
Bmnpv Bacmid

scholarly journals Cloning, Expression and Purification ofBacillus cereusEndochitinase in theEscherichia coliAD494(DE3)pLysS Expression System

2009 ◽  
Vol 73 (5) ◽  
pp. 1172-1174
Author(s):  
Wei-Ming CHEN ◽  
Gen-Hung CHEN ◽  
Ching-San CHEN ◽  
Shann-Tzong JIANG
Keyword(s):  
Expression System ◽  
Expression And Purification

Cloning, Expression, and Purification of Pseudomonas aeruginosa Keratinase in Escherichia coli AD494(DE3)pLysS Expression System

2009 ◽  
Vol 57 (9) ◽  
pp. 3506-3511 ◽  
Author(s):  
Hsin-Hung Lin ◽  
Li-Jung Yin ◽  
Shann-Tzong Jiang
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Expression System ◽  
Expression And Purification

Expression and Purification of Stable 33-kDa Soluble Human CD23 Using the Drosophila S2 Expression System

2001 ◽  
Vol 22 (2) ◽  
pp. 330-336 ◽  
Author(s):  
Sanjay S. Khandekar ◽  
Ruth J. Mayer ◽  
Donna M. Cusimano ◽  
Steven R. Katchur ◽  
Edward R. Appelbaum
Keyword(s):  
Expression System ◽  
Expression And Purification

Database Study on the Expression and Purification of Membrane Proteins

2021 ◽  
Vol 28 ◽  
Author(s):  
Chen-Yan china Zhang ◽  
Shi-Qi Zhao ◽  
Shi-Long Zhang ◽  
Li-Heng Luo ◽  
Ding-Chang Liu ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Protein Expression ◽  
Drug Targets ◽  
Expression System ◽  
Data Bank ◽  
Hek293 Cells ◽  
Expression And Purification ◽  
Beta Barrel ◽  
Membrane Protein Expression

: Membrane proteins are crucial for biological processes, and many of them are important to drug targets. Understanding the three-dimensional structures of membrane proteins are essential to evaluate their bio function and drug design. High-purity membrane proteins are important for structural determination. Membrane proteins have low yields and are difficult to purify because they tend to aggregate. We summarized membrane protein expression systems, vectors, tags, and detergents, which have deposited in the Protein Data Bank (PDB) in recent four-and-a-half years. Escherichia coli is the most expression system for membrane proteins, and HEK293 cells are the most commonly cell lines for human membrane protein expression. The most frequently vectors are pFastBac1 for alpha-helical membrane proteins, pET28a for beta-barrel membrane proteins, and pTRC99a for monotopic membrane proteins. The most used tag for membrane proteins is the 6×His-tag. FLAG commonly used for alpha-helical membrane proteins, Strep and GST for beta-barrel and monotopic membrane proteins, respectively. The detergents and their concentrations used for alpha-helical, beta-barrel, and monotopic membrane proteins are different, and DDM is commonly used for membrane protein purification. It can guide the expression and purification of membrane proteins, thus contributing to their structure and bio function studying.

[15] Expression and purification of G protein βγ subunits using baculovirus expression system

1996 ◽  
pp. 207-226 ◽  
Author(s):  
Stephen G. Graber ◽  
Margaret A. Lindorfer ◽  
James C. Garrison
Keyword(s):  
G Protein ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Expression And Purification ◽  
Baculovirus Expression

Expression and Purification of Vaccinia Virus DNA Topoisomerase IB Produced in the Silkworm–Baculovirus Expression System

2019 ◽  
Vol 61 (8) ◽  
pp. 622-630 ◽  
Author(s):  
Jian Xu ◽  
Jae Man Lee ◽  
Tuneyuki Tatsuke ◽  
Takeru Ebihara ◽  
Akitsu Masuda ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Expression And Purification ◽  
Dna Topoisomerase ◽  
Baculovirus Expression ◽  
Topoisomerase Ib
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