scholarly journals Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Tyler J. Mason ◽  
Harmonie M. Bettenhausen ◽  
Jacqueline M. Chaparro ◽  
Mark E. Uchanski ◽  
Jessica E. Prenni
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Direct Analysis ◽  
Quality Characteristics ◽  
Ambient Mass Spectrometry ◽  
Postharvest Quality ◽  
Ionization Mass ◽  
Ambient Conditions ◽  
Assess Quality

AbstractHorticulturists are interested in evaluating how cultivar, environment, or production system inputs can affect postharvest quality. Ambient mass spectrometry approaches enable analysis of minimally processed samples under ambient conditions and offer an attractive high-throughput alternative for assessing quality characteristics in plant products. Here, we evaluate direct analysis in real time (DART-MS) mass spectrometry and rapid evaporative ionization-mass spectrometry (REIMS) to assess quality characteristics in various pepper (Capsicum annuum L.) cultivars. DART-MS exhibited the ability to discriminate between pod colors and pungency based on chemical fingerprints, while REIMS could distinguish pepper market class (e.g., bell, lunchbox, and popper). Furthermore, DART-MS analysis resulted in the putative detection of important bioactive compounds in human diet such as vitamin C, p-coumaric acid, and capsaicin. The results of this study demonstrate the potential for these approaches as accessible and reliable tools for high throughput screening of pepper quality.

scholarly journals MALDIViz: A Comprehensive Informatics Tool for MALDI-MS Data Visualization and Analysis

2017 ◽  
Vol 22 (10) ◽  
pp. 1246-1252 ◽  
Author(s):  
Kishore Kumar Jagadeesan ◽  
Simon Ekström
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Web Application ◽  
Low Cost ◽  
Maldi Ms ◽  
Ionization Mass ◽  
Label Free ◽  
Label Free Detection ◽  
R Shiny

Recently, mass spectrometry (MS) has emerged as an important tool for high-throughput screening (HTS) providing a direct and label-free detection method, complementing traditional fluorescent and colorimetric methodologies. Among the various MS techniques used for HTS, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides many of the characteristics required for high-throughput analyses, such as low cost, speed, and automation. However, visualization and analysis of the large datasets generated by HTS MALDI-MS can pose significant challenges, especially for multiparametric experiments. The datasets can be generated fast, and the complexity of the experimental data (e.g., screening many different sorbent phases, the sorbent mass, and the load, wash, and elution conditions) makes manual data analysis difficult. To address these challenges, a comprehensive informatics tool called MALDIViz was developed. This tool is an R-Shiny-based web application, accessible independently of the operating system and without the need to install any program locally. It has been designed to facilitate easy analysis and visualization of MALDI-MS datasets, comparison of multiplex experiments, and export of the analysis results to high-quality images.

Flow injection analysis electrospray ionization mass spectrometry for high-throughput screening of a gene library for amidase activity

2009 ◽  
Vol 394 (2) ◽  
pp. 159-163 ◽  
Author(s):  
Huub J.W. Henderickx ◽  
Petronella C. Raemakers-Franken ◽  
Sjoerd van der Wal ◽  
Chris G. de Koster ◽  
Alexander L.L. Duchateau ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
High Throughput ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Electrospray Ionization Mass Spectrometry ◽  
High Throughput Screening ◽  
Ionization Mass ◽  
Gene Library ◽  
Amidase Activity

High-throughput screening of organic reactions in microdroplets using desorption electrospray ionization mass spectrometry (DESI-MS): hardware and software implementation

2020 ◽  
Vol 12 (28) ◽  
pp. 3654-3669
Author(s):  
Tiago Jose P. Sobreira ◽  
Larisa Avramova ◽  
Botond Szilagyi ◽  
David L. Logsdon ◽  
Bradley P. Loren ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
High Throughput ◽  
Electrospray Ionization Mass Spectrometry ◽  
High Throughput Screening ◽  
Desorption Electrospray Ionization ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Desi Ms ◽  
Novel Method

Implementation of a novel method for high-throughput screening of reactions in microdroplets. The reaction and analysis steps are performed simultaneously using desorption electrospray ionization mass spectrometry (DESI-MS) at a rate of up to 1 reaction mixture per second.

High-throughput screening of phthalate-containing objects in the kindergartens by ambient mass spectrometry

2018 ◽  
Vol 1039 ◽  
pp. 65-73 ◽  
Author(s):  
Hung Su ◽  
Min-Zong Huang ◽  
Jo-Han Chou ◽  
Ting-Hao Chang ◽  
Yu-Min Jiang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Ambient Mass Spectrometry
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