scholarly journals Bio-fabrication of silver nanoparticles by phycocyanin, characterization, in vitro anticancer activity against breast cancer cell line and in vivo cytotxicity

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Noura El-Ahmady El-Naggar ◽  
Mervat H. Hussein ◽  
Asmaa Atallah El-Sawah
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line

scholarly journals In vitro antineoplastic activity in triple-negative breast cancer cell line and in vivo

2014 ◽  
Vol 8 (Suppl 4) ◽  
pp. P22
Author(s):  
Klesia Madeira ◽  
Murilo Cerri ◽  
Renata Daltoé ◽  
Alice Herlinger ◽  
João Filho ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cell Line ◽  
Triple Negative ◽  
Cancer Cell Line

scholarly journals Formulation of Piperine–Chitosan-Coated Liposomes: Characterization and In Vitro Cytotoxic Evaluation

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3281
Author(s):  
Syed Sarim Imam ◽  
Sultan Alshehri ◽  
Mohammad A. Altamimi ◽  
Afzal Hussain ◽  
Wajhul Qamar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Therapeutic Efficacy ◽  
Cancer Cell Line ◽  
Permeation Study ◽  
Study Results

The present research work is designed to prepare and evaluate piperine liposomes and piperine–chitosan-coated liposomes for oral delivery. Piperine (PPN) is a water-insoluble bioactive compound used for different diseases. The prepared formulations were evaluated for physicochemical study, mucoadhesive study, permeation study and in vitro cytotoxic study using the MCF7 breast cancer cell line. Piperine-loaded liposomes (PLF) were prepared by the thin-film evaporation method. The selected liposomes were coated with chitosan (PLFC) by electrostatic deposition to enhance the mucoadhesive property and in vitro therapeutic efficacy. Based on the findings of the study, the prepared PPN liposomes (PLF3) and chitosan coated PPN liposomes (PLF3C1) showed a nanometric size range of 165.7 ± 7.4 to 243.4 ± 7.5, a narrow polydispersity index (>0.3) and zeta potential (−7.1 to 29.8 mV). The average encapsulation efficiency was found to be between 60 and 80% for all prepared formulations. The drug release and permeation study profile showed biphasic release behavior and enhanced PPN permeation. The in vitro antioxidant study results showed a comparable antioxidant activity with pure PPN. The anticancer study depicted that the cell viability assay of tested PLF3C2 has significantly (p < 0.001)) reduced the IC50 when compared with pure PPN. The study revealed that oral chitosan-coated liposomes are a promising delivery system for the PPN and can increase the therapeutic efficacy against the breast cancer cell line.

scholarly journals Identifikasi Ekspresi Gen Myc pada Subkultur MCF-7 Breast Cancer Cell Line dengan Sel Punca

2018 ◽  
Vol 7 (3) ◽  
pp. 424
Author(s):  
Yuastika Puspita Sari ◽  
Hirowati Ali ◽  
Aswiyanti Asri
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Luminal A ◽  
Post Hoc ◽  
Mcf 7

Kanker payudara yang paling banyak ditemukan adalah subtipe luminal A dengan karakteristik Estrogen Reseptor+. Subjek penelitian akan diwakili oleh cell line MCF-7 dan Umbilical Cord Blood Mesenchymal Stem Cell (UCBMSC). Over expression Myc pada kanker payudara mengakibatkan sel kanker menjadi lebih invasif dan terjadi resistensi terhadap terapi hormonal. Tujuan penelitian ini adalah mengidentifikasi ekspresi gen Myc pada cell line MCF-7 sebelum dan sesudah pemberian sel punca. Penelitian ini menggunakan desain experimental secara in vitro. Sampel menggunakan MCF-7 dan sel punca yang dibagi menjadi 4 kelompok, yaitu K1 (kelompok kontrol MCF-7), K2 (kelompok kontrol sel punca), P1 (perlakuan subkultur MCF-7 dengan sel punca inkubasi 24 jam), dan P2 (inkubasi 48 jam). Kemudian, dilakukan isolasi RNA, sintesis cDNA, dan ekspresi Myc diperiksa menggunakan PCR dan elektroforesis. Analisa data yang digunakan adalan One Way ANOVA dan post-hoc LSD. Hasil analisis bivariat didapatkan p<0,05. Dari uji post-hoc LSD tidak ditemukan perbedaan yang bermakna antara K1 dengan P1, K1 dengan P2, dan K2 dengan P2. Namun, tetap ditemukan perbedaan yang bermakna antara K2 dengan P1 dan P1 dengan P2. Simpulan penelitian ini adalah tidak terdapat perbedaan bermakna ekspresi gen Myc pada subkultur antara MCF-7 breast cancer cell line dengan pemberian sel punca mesenkimal.

scholarly journals Anticancer activity of THMPP: Downregulation of PI3K/ S6K1 in breast cancer cell line

2020 ◽  
Vol 28 (4) ◽  
pp. 495-503 ◽  
Author(s):  
Suresh Palanivel ◽  
Akshaya Murugesan ◽  
Olli Yli-Harja ◽  
Meenakshisundaram Kandhavelu
Keyword(s):  
Breast Cancer ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line

scholarly journals CYTOTOXIC EFFECT OF SYNTHESIZED SILVER NANOPARTICLES BY PULSE LASER ABLATION ON BREAST CANCER CELL LINE (AMJ13) FROM IRAQI PATIENT AND NORMAL HUMAN LYMPHOCYTES

2018 ◽  
Vol 11 (4) ◽  
pp. 375 ◽  
Author(s):  
Nawfal N R Alrawi ◽  
Mohammed Q Al-ani ◽  
Nahi Y Yaseen
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cell Line ◽  
Dna Fragmentation ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast

 Objective: Synthesized silver nanoparticles (AgNPs) in liquids were investigated as anticancer cells in the present study. Cytotoxic activities of six different concentrations 0.78, 1.56, 3.125, 6.25, 12.5, and 25 μg/ml of AgNPs against human breast cancer cell line (AMJ13) and lymphocytes were assessed with MTT assay.Methods: A Q-Switched Nd: YAG pulsed laser (λ=1064 nm, 800 mJ/pulse) was used for ablation of a pure silver plate to synthesis AgNPs in the polyvinylpyrrolidone and deionize distilled water. Ultraviolet-visible spectroscopy confirmed the synthesis of AgNPs and zeta potential was evaluated. Morphology and size were analyzed by transmission electron microscope. AgNPs concentrations were determined by atomic absorption spectroscopy. Possibilities of apoptosis induction were confirmed using mitochondrial membrane potential assay, DNA fragmentation assay, and glutathione (GSH) assay.Results: The results indicated that AgNPs were able to induce an inhibition of AMJ13 cells compared their damaging effect toward normal lymphocytes were at minimal according to viability with MTT assay.. Furthermore, these results suggested that AgNPs-induced mitochondrial-mediated apoptosis cause DNA fragmentation, but no significant change in GSH level in AMJ13 cells.Conclusions: The overall results indicated that the physically synthesized AgNPs were exhibited dose-dependent cell death in AMJ13 breast cancer cell line, while the effect of AgNPs on lymphocytes was very low, suggesting that physically synthesized AgNPs might be a potential alternative agent for human breast cancer therapy.

Sign in / Sign up

Export Citation Format

Share Document