scholarly journals A pan-specific antiserum produced by a novel immunization strategy shows a high spectrum of neutralization against neurotoxic snake venoms

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Kavi Ratanabanangkoon ◽  
Kae Yi Tan ◽  
Kritsada Pruksaphon ◽  
Chaiya Klinpayom ◽  
José María Gutiérrez ◽  
...  
Author(s):  
J. P. Petrali ◽  
E. J. Donati ◽  
L. A. Sternberger

Specific contrast is conferred to subcellular antigen by applying purified antibodies, exhaustively labeled with uranium under immunospecific protection, to ultrathin sections. Use of Seligman’s principle of bridging osmium to metal via thiocarbohydrazide (TCH) intensifies specific contrast. Ultrathin sections of osmium-fixed materials were stained on the grid by application of 1) thiosemicarbazide (TSC), 2) unlabeled specific antiserum, 3) uranium-labeled anti-antibody and 4) TCH followed by reosmication. Antigens to be localized consisted of vaccinia antigen in infected HeLa cells, lysozyme in monocytes of patients with monocytic or monomyelocytic leukemia, and fibrinogen in the platelets of these leukemic patients. Control sections were stained with non-specific antiserum (E. coli).In the vaccinia-HeLa system, antigen was localized from 1 to 3 hours following infection, and was confined to degrading virus, the inner walls of numerous organelles, and other structures in cytoplasmic foci. Surrounding architecture and cellular mitochondria were unstained. 8 to 14 hours after infection, antigen was localized on the outer walls of the viral progeny, on cytoplasmic membranes, and free in the cytoplasm. Staining of endoplasmic reticulum was intense and focal early, and weak and diffuse late in infection.


1967 ◽  
Vol 56 (1_Suppl) ◽  
pp. S122
Author(s):  
P.-J. Czygan ◽  
D. Krebs ◽  
F. Lehmann ◽  
G. Bettendorf

2007 ◽  
Vol 12 (02) ◽  
Author(s):  
A. Terceño Gómez ◽  
A. Fernández Bariviera ◽  
J. M. Brotons Martí­nez

Pathology ◽  
1981 ◽  
Vol 13 (1) ◽  
pp. 13-27 ◽  
Author(s):  
Struan K. Sutherland ◽  
A.R. Coulter ◽  
R.D. Harris ◽  
K.E. Lovering ◽  
I.D. Roberts

2010 ◽  
Vol 30 (7) ◽  
pp. 1775-1777
Author(s):  
Peng JI ◽  
Hong-wei GE

1999 ◽  
Vol 64 (8) ◽  
pp. 1211-1252 ◽  
Author(s):  
Jan Hlaváček ◽  
Renáta Marcová

The first part of this review deals with the biosynthesis and a biological function of strongly vasoactive peptides named endothelins (ETs) including vasoactive intestinal contractor. Where it was useful, snake venoms sarafotoxins which are structural endothelin derivatives, were also mentioned. In the second part, an attention is paid to structural basis of the ETs biological activity, with respect to alterations of amino acid residues in the parent peptides modifying the conformation and consequently the physico-chemical and biological properties in corresponding ETs analogs. Special attention is focussed on the area of ETs receptors and their interaction with peptide and non peptide agonists and antagonists, important in designing selective inhibitors of ETs receptors potentially applicable as drugs in a medicine. A review with 182 references.


Sign in / Sign up

Export Citation Format

Share Document