scholarly journals Impact of endoscopic submucosal dissection and epithelial cell sheet engraftment on systemic cytokine dynamics in patients with oesophageal cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Akira Yoshida ◽  
Tomoaki Takata ◽  
Tsutomu Kanda ◽  
Naoyuki Yamaguchi ◽  
Hitomi Minami ◽  
...  
Keyword(s):  
Endoscopic Submucosal Dissection ◽  
Inflammatory Cytokines ◽  
Oesophageal Cancer ◽  
Cell Sheet ◽  
Major Complication ◽  
Tumour Microenvironment ◽  
Oesophageal Stricture ◽  
Submucosal Dissection ◽  
Autologous Cell ◽  
Cytokine Levels

AbstractThe tumour microenvironment (TME) plays an important role in cancer development, progression, and metastasis. Various cytokines are present in the TME in oesophageal cancer. Oesophageal stricture is a major complication of endoscopic submucosal dissection (ESD) for oesophageal cancer, and inflammatory cytokines are closely related to its pathogenesis. However, the cytokine crosstalk involved in the oesophageal cancer TME and post-ESD stricture has not been fully elucidated. This study investigated the comprehensive cytokine dynamics following ESD in patients with oesophageal cancer. In addition, the effect of a novel preventive technique for post-ESD stricture, autologous cell sheet engraftment, on cytokine levels was evaluated. Various pro-inflammatory and anti-tumorigenic cytokines were elevated in patients with oesophageal cancer, and ESD transiently influenced cytokine concentrations. IL-1β and TNF-α, two major pro-inflammatory cytokines that induce oesophageal stricture, were significantly suppressed by cell sheet engraftment. In conclusion, this study revealed the distinct cytokine dynamics after ESD in patients with oesophageal cancer, together with the effect of autologous cell sheet engraftment on cytokine fluctuation. These results can accelerate research on the TME and therapeutic strategies for oesophageal cancer.

scholarly journals The Efficacy of Autologous Myoblast Sheet Transplantation to Prevent Perforation After Duodenal Endoscopic Submucosal Dissection in Porcine Model

2020 ◽  
Vol 29 ◽  
pp. 096368972096388
Author(s):  
Ryo Matsumoto ◽  
Kengo Kanetaka ◽  
Yasuhiro Maruya ◽  
Shun Yamaguchi ◽  
Shinichiro Kobayashi ◽  
...  
Keyword(s):  
Endoscopic Submucosal Dissection ◽  
Porcine Model ◽  
Histopathological Examination ◽  
Abdominal Cavity ◽  
Cell Sheet ◽  
Duodenal Wall ◽  
Thin Wall ◽  
Submucosal Dissection ◽  
Lethal Complications ◽  
Myoblast Cell

The recent advent of endoscopy has enabled the endoscopic submucosal dissection (ESD) of superficial nonampullary duodenal epithelial tumors. However, the substantially thin wall and presence of bile and pancreatic juice make it technically difficult to perform duodenal ESD without perforation, which leads to lethal complications. The present study evaluated the efficacy of autologous myoblast sheet transplantation for the prevention of late perforation after duodenal ESD in a porcine model. Two weeks before ESD, skeletal muscle was surgically excised from the femur of pigs, and myoblasts were isolated and seeded in temperature-responsive culture dishes to prepare sheets. Immediately after ESD, the autologous myoblast sheets were attached to the serosal surface at the ESD site with omentopexy. The pigs were divided into two groups: the autologous myoblast sheet group ( n = 5), where the myoblast cell sheet was attached to the ESD ulcer part from the duodenal serous side, and the Omentum group ( n = 5), where only the omentum was used. The pigs were sacrificed and analyzed macroscopically and histologically on postoperative day 3. The macroscopic examination of the abdominal cavity revealed perforation in the ESD ulcer area and leakage of bile in the Omentum group but no perforation in the Sheet group. A histopathological examination revealed that continuity of the duodenal wall at the ESD site was maintained with dense connective tissue in the Sheet group. In conclusion, autologous myoblast sheets were useful for preventing perforation after duodenal ESD.

scholarly journals Prevention and Treatment of Esophageal Stenosis after Endoscopic Submucosal Dissection for Early Esophageal Cancer

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Jing Wen ◽  
Zhongsheng Lu ◽  
Qingsen Liu
Keyword(s):  
Endoscopic Submucosal Dissection ◽  
Short Review ◽  
Esophageal Stenosis ◽  
Esophageal Dilatation ◽  
Early Esophageal Cancer ◽  
Submucosal Dissection ◽  
Autologous Cell ◽  
Prevention And Treatment ◽  
Mucosal Lesions ◽  
Autologous Cell Transplantation

Endoscopic submucosal dissection (ESD) for the treatment of esophageal mucosal lesions is associated with a risk of esophageal stenosis, especially for near-circumferential or circumferential esophageal mucosal defects. Here, we review historic and modern studies on the prevention and treatment of esophageal stenosis after ESD. These methods include prevention via pharmacological treatment, endoscopic autologous cell transplantation, endoscopic esophageal dilatation, and stent placement. This short review will focus on direct prevention and treatment, which may help guide the way forward.

Application of cell sheet technology for esophageal endoscopic submucosal dissection

2011 ◽  
Vol 13 (1) ◽  
pp. 105-109 ◽  
Author(s):  
Takeshi Ohki ◽  
Masayuki Yamamoto ◽  
Masaho Ota ◽  
Teruo Okano ◽  
Masakazu Yamamoto
Keyword(s):  
Endoscopic Submucosal Dissection ◽  
Cell Sheet ◽  
Submucosal Dissection ◽  
Cell Sheet Technology

scholarly journals The Most Influential Publications on Endoscopic Submucosal Dissection: A Bibliometric Analysis

2020 ◽  
Vol 20 (4) ◽  
pp. 306-317
Author(s):  
Chang Seok Bang ◽  
Jae Jun Lee ◽  
Gwang Ho Baik
Keyword(s):  
Endoscopic Submucosal Dissection ◽  
Meta Analysis ◽  
Cell Sheet ◽  
Gastrointestinal Neoplasms ◽  
Submucosal Dissection ◽  
Submucosal Tunnel ◽  
Endoscopic Closure ◽  
Management Of Complications ◽  
Detailed List ◽  
Number Of Citations

Background/Aims: Endoscopic submucosal dissection (ESD) is the first-line treatment for superficial gastrointestinal neoplasms with negligible lymph node metastasis. It has evolved through improvements in expertise and equipment, increased understanding of indications and short- and long-term outcomes, and better management of complications. This study aimed to assess and characterize the most influential publications in ESD research.Materials and Methods: We searched the top 50 most cited articles using Web of Science Core Collection (WoSCC) and Google Scholar (GS) from the inception of these services to January 2019. The top 50 Altmetric Attention Score (AAS) articles based on online media mentions were also searched. Each article was evaluated for the number of citations, title, journal, and publication year.Results: The number of citations for the top 50 WoSCC articles on ESD ranged from 37 to 199; Endoscopy published the most articles (20%). Among the top 50 GS articles, Gastrointestinal Endoscopy published the most ESD articles (34%) and the most shared AAS articles (42.6%). PubMed Central article citations in WoSCC or GS showed significant correlation with those from each metric, unlike AAS. The words with the highest relevance scores were “submucosal tunnel dissection,” “guideline,” “novel submucosal gel,” “adhesive material,” “cell sheet,” “esophageal ulcer,” “hemospray,” and “endoscopic closure,” while the following words were influential: “meta-analysis,” “esophageal stricture,” “perforation,” “bleeding,” “fibrin glue,” “artificial ulcer,” “porcine model” and “esophageal squamous cell neoplasia,” excluding “ESD.”Conclusions: This study presents a detailed list of influential articles, journals, and topic words.

scholarly journals Cell Sheet Transplantation for Esophageal Stricture Prevention after Endoscopic Submucosal Dissection in a Porcine Model

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0148249 ◽  
Author(s):  
Guillaume Perrod ◽  
Gabriel Rahmi ◽  
Laetitia Pidial ◽  
Sophie Camilleri ◽  
Alexandre Bellucci ◽  
...  
Keyword(s):  
Endoscopic Submucosal Dissection ◽  
Esophageal Stricture ◽  
Porcine Model ◽  
Cell Sheet ◽  
Submucosal Dissection

scholarly journals Impact of electrosurgical unit mode on post esophageal endoscopic submucosal dissection stricture in an in vivo porcine model

2018 ◽  
Vol 06 (03) ◽  
pp. E376-E381 ◽  
Author(s):  
Yusuke Tonai ◽  
Ryu Ishihara ◽  
Yasushi Yamasaki ◽  
Masamichi Arao ◽  
Taro Iwatsubo ◽  
...  
Keyword(s):  
Endoscopic Submucosal Dissection ◽  
Porcine Model ◽  
Major Complication ◽  
Scar Formation ◽  
Submucosal Dissection ◽  
Bipolar Mode ◽  
Mucosal Incision ◽  
Superficial Esophageal Carcinoma ◽  
Electrosurgical Unit

Abstract Background and aim Strictures are a major complication of esophageal endoscopic submucosal dissection (ESD) for superficial esophageal carcinoma. Post ESD, stricture develops during the process of scar formation, which is related to inflammation caused by ESD. We planned a study to evaluate whether certain electrosurgical unit modes could attenuate strictures after esophageal ESD. Methods A total of 16 ESD, three-quarters of the esophageal circumference, were performed in four live pigs. A ball-tip Flush knife was used for mucosal incision. Submucosal dissection was performed using a Hook knife in monopolar mode and a ball-tip Jet B-knife in bipolar mode. Applied electrosurgical unit modes were FORCED COAG, SWIFT COAG, SPRAY COAG, ENDO CUT in monopolar mode, and FORCED COAG in bipolar mode. One month after ESD, the pigs were killed humanely and the severity of strictures and fibrosis was assessed. Results The resected site in the esophagus showed complete mucosal regrowth and scar formation in all pigs. The quotients of stricture following ENDO CUT, SWIFT COAG, FORCED COAG effect2, FORCED COAG effect3, FORCED COAG effect4, SPRAY COAG, and Bipolar FORCED COAG mode were 16 %, 28 %, 38 %, 33 %, 51 %, 39 %, and 47 %, respectively. The equivalent quotients of fibrosis were 7 %, 28 %, 31 %, 30 %, 35 %, 63 %, and 100 %, respectively. ENDO CUT mode was associated with the lowest mean quotients of stricture and fibrosis. Conclusion ENDO CUT mode showed promising results to attenuate fibrosis and strictures after esophageal ESD.

scholarly journals Analysis of Delayed Bleeding after Endoscopic Submucosal Dissection for Gastric Epithelial Neoplasms

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Shinichi Mukai ◽  
Songde Cho ◽  
Takahiro Kotachi ◽  
Akinori Shimizu ◽  
Genta Matuura ◽  
...  
Keyword(s):  
Endoscopic Submucosal Dissection ◽  
Preventive Measures ◽  
Univariate Analysis ◽  
Major Complication ◽  
Emergency Endoscopy ◽  
Tumor Depth ◽  
Delayed Bleeding ◽  
Submucosal Dissection ◽  
Epithelial Neoplasms

Aim. Delayed bleeding after endoscopic submucosal dissection (ESD) for gastric epithelial neoplasms is a major complication. We investigated factors related to post-ESD bleeding to identify preventive measures.Methods. The study included 161 gastric epithelial neoplasms in 142 patients from June 2007 to September 2010. Post-ESD bleeding was defined as an ulcer with active bleeding or apparent exposed vessels diagnosed by an emergency endoscopy or a planned follow-up endoscopy. We analyzed associations between bleeding and the following factors: age, sex, morphology, pathology, tumor depth, ulcer presence/absence, location, size of the resected lesion, duration of the procedure, the number of times bleeding occurred during ESD, and the use of anticoagulants and/or antiplatelet drugs. Subsequently, we examined characteristics of bleeding cases.Results. Post-ESD bleeding occurred in 21 lesions. Univariate analysis of these cases showed that ulcer presence/absence (P<0.001), middle or lower third lesions (P=0.036), circumference (P=0.014), and a post-ESD ulcer with an extended lesser curve (P=0.009) were significant predictors of bleeding. Multivariate analysis showed that ulcer presence/absence (OR 9.73, 95% CI 2.28–41.53) was the only significant predictor.Conclusion. Ulcer presence/absence was considered the most significant predictor of post-ESD bleeding.

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