scholarly journals Reactivation of latent tuberculosis through modulation of resuscitation promoting factors by diabetes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Arpana Verma ◽  
Maninder Kaur ◽  
Lakshya Veer Singh ◽  
Divya Aggarwal ◽  
Indu Verma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Histopathological Changes ◽  
Altered Expression ◽  
Colony Forming Unit ◽  
Latently Infected ◽  
Resuscitation Promoting Factors

AbstractThe evidence of an association between diabetes and latent tuberculosis infection (LTBI) remains limited and inconsistent. Thus, the study aims to delineate the role of diabetes in activation of latent tuberculosis infection. Murine model of latent tuberculosis and diabetes was developed, bacillary load and gene expression of resuscitation promoting factors (rpfA-E) along with histopathological changes in the lungs and spleen were studied. Treatment for LTBI [Rifampicin (RIF) + Isoniazid (INH)] was also given to latently infected mice with or without diabetes for 4 weeks. Diabetes was found to activate latent tuberculosis as the colony forming unit (CFU) counts were observed to be > 104 in lungs and spleen. The gene expression of hspX was downregulated and that of rpfB and rpfD was observed to be upregulated in latently infected mice with diabetes compared to those without diabetes. However, no significant reduction in the CFU counts was observed after 4 weeks of treatment with RIF and INH. Diabetes helps in the progression of LTBI to active disease mainly through altered expression of resuscitation promoting factors rpfB and rpfD, which can serve as important targets to reduce the shared burden of tuberculosis and diabetes.

PLACE OF IGRA TESTS (TESTS FOR INTERFERON-GAMMA DETERMINATION) IN TUBERCULOSIS INFECTION DIAGNOSIS

2020 ◽  
Vol 99 (6) ◽  
pp. 231-235
Author(s):  
O.E. Russkikh ◽  
◽  
D.A. Kudlay ◽  
◽  
Keyword(s):  
Interferon Gamma ◽  
Significant Role ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Scientific Data ◽  
Tuberculosis Infection ◽  
Population Groups ◽  
Infection Diagnosis ◽  
Immunological Tests

The review presents scientific data available to date on the concept of the emergence and spread of latent tuberculosis infection (TI) in the current tuberculosis epidemic situation in society. Information on modern immunological tests for TI diagnosis is presented and the significant role of IGRA tests in the diagnostic tactics of detecting latent TI in various population groups is determined.

scholarly journals Gene expression tryptophan aspartate coat protein in determining latent tuberculosis infection using immunocytochemistry and real time polimerase chain reaction

2020 ◽  
Author(s):  
Rebekah J. Setiabudi ◽  
Ni Made Mertaniasih ◽  
Muhammad Amin ◽  
Wayan Tunas Artama
Keyword(s):  
Gene Expression ◽  
Mycobacterium Tuberculosis ◽  
Coat Protein ◽  
Real Time ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Peripheral Blood Monocyte ◽  
Tuberculosis Infection ◽  
Chain Reaction ◽  
The Difference

Background: Tuberculosis (TB) remains a major cause of morbidity and mortality worldwide. Problem of Latent Tuberculosis Infection (LTBI) is increasing in number especially in countries with high TB incidence rate, such as Indonesia. Although not every LTBI will become active TB, if untreated and not handled appropriately it can still be a source of transmission and may increase the rate of resistance to the first-line TB drugs. Mycobacterium tuberculosis as a cause of tuberculosis disease is an intracellular pathogens that survives within the phagosome of host macrophages. Several host factors are involved in this process, including the Tryptophan Aspartate-containing Coat Protein (TACO). TACO is a protein recruited and retained by viable Mycobacterium tuberculosis on the surface of the phagosome membrane to maintain its survival in phagosome, because the presence of TACO plays an important role in inhibiting the fusion of phagosomes and lysosomes. Objective: the aim of this studyis to assess the difference of gene expression TACO protein in Latent Tuberculosis Infection (LTBI) and healthy people. Method: A preliminary studyof mRNA examination of TACO protein using Immunocytochemistry (ICC) and Real Time-Polimerase Chain Reaction (RT-PCR) method by a PCR Light Cycler 2.0 machine (Roche) in LTBI and healthy groups. Results: 18 samples of peripheral blood monocyte cells (PBMCs) were collected and divided into 2 groups. We found that there was a significantly difference between the 2 groups of samples. Conclusion: Further research is required to consider that the measurement of TACO expression using RT-PCRcan used as one of the other method to determine LTBI.

scholarly journals The Role of Interferon-gamma Release Assay in Tuberculosis Control

2012 ◽  
Vol 63 (1) ◽  
pp. 49-59 ◽  
Author(s):  
Anamarija Jurčev-Savičević ◽  
Vera Katalinić-Janković ◽  
Kornelija Miše ◽  
Ivan Gudelj
Keyword(s):  
Interferon Gamma ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Interferon Gamma Release Assay ◽  
Tuberculosis Control ◽  
Aggressive Approach ◽  
Release Assay ◽  
Low Incidence

The Role of Interferon-gamma Release Assay in Tuberculosis ControlTuberculosis is still one of the major global public health threats. Countries with low incidence must focus on exhausting the reservoir of future cases by preventing reactivation. Therefore, it is important to identify and effectively treat those individuals who have latent tuberculosis infection and who may develop active disease. The tuberculin skin test has been the standard for detection of immune response against M. tuberculosis since the beginning of the 20th century. The new millennium has brought advancement in the diagnosis of latent tuberculosis infection. The name of the new blood test is interferon-gamma release assay (IGRA). Croatia is a middle-incidence country with a long decreasing trend and developed tuberculosis control. To reach low incidence and finally eliminate tuberculosis, its tuberculosis programme needs a more aggressive approach that would include intensive contact investigation and treatment of persons with latent tuberculosis infection. This article discusses the current uses of IGRA and its role in tuberculosis control.

scholarly journals Role of QuantiFERON-TB Gold In-Tube test in detection of latent tuberculosis infection in health care contacts in Alexandria, Egypt

2014 ◽  
Vol 8 (12) ◽  
pp. 1310-1317
Author(s):  
Abdel Aziz Amer Safaa ◽  
Mahmoud El Hefnawy Azza ◽  
Abdel Hameed Harfoush Reem ◽  
Samy Saad Mona
Keyword(s):  
Health Care ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Tube Test
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