scholarly journals Author Correction: The promise(s) of mesenchymal stem cell therapy in averting preclinical diabetes: lessons from in vivo and in vitro model systems

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nagasuryaprasad Kotikalapudi ◽  
Samuel Joshua Pragasam Sampath ◽  
Sinha Sukesh Narayan ◽  
Bhonde R. ◽  
Harishankar Nemani ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
In Vitro Model ◽  
Model Systems ◽  
Mesenchymal Stem Cell Therapy ◽  
Preclinical Diabetes ◽  
Vitro Model

scholarly journals The promise(s) of mesenchymal stem cell therapy in averting preclinical diabetes: lessons from in vivo and in vitro model systems

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nagasuryaprasad Kotikalapudi ◽  
Samuel Joshua Pragasam Sampath ◽  
Sinha Sukesh Narayan ◽  
Bhonde Ramesh R ◽  
Harishankar Nemani ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Clinical Success ◽  
Significant Risk ◽  
Stromal Vascular Fraction ◽  
Significant Risk Factor ◽  
Model Systems ◽  
Mesenchymal Stem Cell Therapy

AbstractObesity (Ob) poses a significant risk factor for the onset of metabolic syndrome with associated complications, wherein the Mesenchymal Stem Cell (MSC) therapy shows pre-clinical success. Here, we explore the therapeutic applications of human Placental MSCs (P-MSCs) to address Ob-associated Insulin Resistance (IR) and its complications. In the present study, we show that intramuscular injection of P-MSCs homed more towards the visceral site, restored HOMA-IR and glucose homeostasis in the WNIN/GR-Ob (Ob-T2D) rats. P-MSC therapy was effective in re-establishing the dysregulated cytokines. We report that the P-MSCs activates PI3K-Akt signaling and regulates the Glut4-dependant glucose uptake and its utilization in WNIN/GR-Ob (Ob-T2D) rats compared to its control. Our data reinstates P-MSC treatment's potent application to alleviate IR and restores peripheral blood glucose clearance evidenced in stromal vascular fraction (SVF) derived from white adipose tissue (WAT) of the WNIN/GR-Ob rats. Gaining insights, we show the activation of the PI3K-Akt pathway by P-MSCs both in vivo and in vitro (palmitate primed 3T3-L1 cells) to restore the insulin sensitivity dysregulated adipocytes. Our findings suggest a potent application of P-MSCs in  pre-clinical/Ob-T2D management.

scholarly journals Coupling primary and stem cell–derived cardiomyocytes in an in vitro model of cardiac cell therapy

2016 ◽  
Vol 213 (3) ◽  
pp. 2133OIA11
Author(s):  
Yvonne Aratyn-Schaus ◽  
Francesco S. Pasqualini ◽  
Hongyan Yuan ◽  
Megan L. McCain ◽  
George J.C. Ye ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Therapy ◽  
In Vitro Model ◽  
Cardiac Cell ◽  
Cardiac Cell Therapy ◽  
Vitro Model

scholarly journals Coupling primary and stem cell–derived cardiomyocytes in an in vitro model of cardiac cell therapy

2016 ◽  
Vol 212 (4) ◽  
pp. 389-397 ◽  
Author(s):  
Yvonne Aratyn-Schaus ◽  
Francesco S. Pasqualini ◽  
Hongyan Yuan ◽  
Megan L. McCain ◽  
George J.C. Ye ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Therapy ◽  
In Vitro Model ◽  
Cell Junction ◽  
Cardiac Cell ◽  
Force Transmission ◽  
Cardiac Cell Therapy ◽  
Vitro Model ◽  
Cell Cell

The efficacy of cardiac cell therapy depends on the integration of existing and newly formed cardiomyocytes. Here, we developed a minimal in vitro model of this interface by engineering two cell microtissues (μtissues) containing mouse cardiomyocytes, representing spared myocardium after injury, and cardiomyocytes generated from embryonic and induced pluripotent stem cells, to model newly formed cells. We demonstrated that weaker stem cell–derived myocytes coupled with stronger myocytes to support synchronous contraction, but this arrangement required focal adhesion-like structures near the cell–cell junction that degrade force transmission between cells. Moreover, we developed a computational model of μtissue mechanics to demonstrate that a reduction in isometric tension is sufficient to impair force transmission across the cell–cell boundary. Together, our in vitro and in silico results suggest that mechanotransductive mechanisms may contribute to the modest functional benefits observed in cell-therapy studies by regulating the amount of contractile force effectively transmitted at the junction between newly formed and spared myocytes.

scholarly journals Imaging cell biology in live animals: Ready for prime time

2013 ◽  
Vol 201 (7) ◽  
pp. 969-979 ◽  
Author(s):  
Roberto Weigert ◽  
Natalie Porat-Shliom ◽  
Panomwat Amornphimoltham
Keyword(s):  
Cell Biology ◽  
Cancer Biology ◽  
In Vitro Model ◽  
Time Lapse ◽  
Living Cells ◽  
Model Systems ◽  
Prime Time ◽  
Vitro Model

Time-lapse fluorescence microscopy is one of the main tools used to image subcellular structures in living cells. Yet for decades it has been applied primarily to in vitro model systems. Thanks to the most recent advancements in intravital microscopy, this approach has finally been extended to live rodents. This represents a major breakthrough that will provide unprecedented new opportunities to study mammalian cell biology in vivo and has already provided new insight in the fields of neurobiology, immunology, and cancer biology.

268 An in vivo-like tumor stem cell-related glioblastoma in vitro model for drug discovery

2010 ◽  
Vol 8 (7) ◽  
pp. 87
Author(s):  
S.S. Jensen ◽  
C. Aaberg-Jessen ◽  
A. Nørregaard ◽  
B.W. Kristensen
Keyword(s):  
Stem Cell ◽  
Drug Discovery ◽  
In Vitro Model ◽  
Tumor Stem Cell ◽  
Vitro Model
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