Desorption electrospray ionization mass spectroscopy (DESI-MS) imaging of brain tumors

2010 ◽  
Vol 3 (29) ◽  
pp. 910-910
Keyword(s):  
Brain Tumors ◽  
Electrospray Ionization ◽  
Mass Spectroscopy ◽  
Desorption Electrospray Ionization ◽  
Ionization Mass ◽  
Electrospray Ionization Mass Spectroscopy ◽  
Ms Imaging ◽  
Ionization Mass Spectroscopy ◽  
Desi Ms

Solvation of Lithium Ions in Mixed Organic Electrolyte Solutions by Electrospray Ionization Mass Spectroscopy

2002 ◽  
Vol 149 (8) ◽  
pp. A1045 ◽  
Author(s):  
Yoshiharu Matsuda ◽  
Tsuyoshi Fukushima ◽  
Hiroyuki Hashimoto ◽  
Ryuichi Arakawa
Keyword(s):  
Electrospray Ionization ◽  
Mass Spectroscopy ◽  
Electrolyte Solutions ◽  
Organic Electrolyte ◽  
Ionization Mass ◽  
Lithium Ions ◽  
Electrospray Ionization Mass Spectroscopy ◽  
Ionization Mass Spectroscopy

291 THE USE OF THE DYNAMIC IMPACT APPROACH AND DESORPTION ELECTROSPRAY IONIZATION - MASS SPECTROSCOPY TO ANALYZE ADIPOGENESIS IN PORCINE ADIPOSE-DERIVED STEM CELLS

2013 ◽  
Vol 25 (1) ◽  
pp. 293 ◽  
Author(s):  
M. Bionaz ◽  
C. R. Ferreira ◽  
E. Monaco ◽  
R. G. Cooks ◽  
M. B. Wheeler
Keyword(s):  
Lipid Profile ◽  
Adipogenic Differentiation ◽  
Desorption Electrospray Ionization ◽  
Ionization Mass ◽  
Adipose Derived Stem Cells ◽  
Dynamic Impact ◽  
Electrospray Ionization Mass Spectroscopy ◽  
Ionization Mass Spectroscopy ◽  
Transcriptomics Data ◽  
Desi Ms

The measurement of end products of metabolism in combination with the dynamic measurement of the transcriptome can be a valuable approach to study complex processes, such as adipogenesis. For this purpose, we have combined the analysis of metabolic pathways using transcriptomics data with in situ lipid imaging obtained by desorption electrospray ionization–mass spectroscopy (DESI-MS). The transcriptome data was obtained from a previous experiment where adipose-derived stem cells (ASC) from 3 individual pigs were induced to differentiate into adipocytes for 21 days. The metabolic pathway analysis of transcriptomics data was performed using the dynamic impact approach (DIA) to determine the directional impact of pathways and gene categories. For the DESI-MS, porcine ASC were induced towards adipogenic differentiation (Monaco et al. 2009 Open Tissue Eng. Regen. Med. J. 2, 20–33) for 2 weeks in 24-well plates over round coverslips. Direct lipid profile of fatty acids and phospholipids in 3 different locations in undifferentiated and differentiated ASC was performed. Relative values of ion counts in each location were used for statistical analysis by means of a two-tailed t-test with unequal variance and false discovery rate (FDR) correction. The DIA uncovered amino acid and lipid metabolism as being the most affected pathways during adipogenesis. Among lipid-related pathways, the DIA indicated a strong induction during differentiation of unsaturation of fatty acids (FA) and metabolism of lipids, particularly triacylglycerol formation (TAG). The metabolism of tryptophan, phenylalanine, and histidine was strongly induced among amino acid-related metabolism. Among the lipid molecules identified in the mass-to-charge ratio (m/z) range of 250–1000 by the DESI-MS, >200 had significantly (FDR <0.01) lower abundance and ~30 lipids had higher abundance in differentiated compared with undifferentiated ASC. The lipid profile of undifferentiated cells was featured by a large amount of lipid molecules in the lower (m/z 780) mass range but with almost no signal in the intermediate range. The differentiated cells had a consistent large increase in amount of molecules in the range from m/z 500 to 650 and a decreased amount in the lower and upper ranges. Among the lipid molecules that decreased during differentiation, we identified several phospholipids, particularly phosphoserine and phosphoinositol species, but also linoleic, linolenic, arachidic, and eicosapentaenoic acids. The unsaturated FA oleate and docosahexaenate increased >10-fold and 2.2-fold, respectively, due to adipogenic differentiation. High-resolution experiments are being performed in order to annotate more, if not all, lipid species identified in the samples by DESI-MS. The DIA indicated greater unsaturation of FA during adipogenesis, confirmed by DESI-MS, which corresponds to the characteristic increase in lipid fractions with intermediate molecular mass. This can be considered a signature of adipogenesis in porcine ASC and essential for TAG formation. The DESI-MS appears to be a valuable tool for rapid lipid profile of cells and with the potential to be used to identify cell differentiation.

scholarly journals Identification of Phenolic Compounds in Aquilaria crassna Leaves Via Liquid Chromatography-Electrospray Ionization Mass Spectroscopy

2012 ◽  
Vol 18 (2) ◽  
pp. 259-262 ◽  
Author(s):  
Tetsuro ITO ◽  
Mamoru KAKINO ◽  
Shigemi TAZAWA ◽  
Masayoshi OYAMA ◽  
Hiroe MARUYAMA ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Electrospray Ionization ◽  
Mass Spectroscopy ◽  
Ionization Mass ◽  
Electrospray Ionization Mass Spectroscopy ◽  
Ionization Mass Spectroscopy ◽  
Aquilaria Crassna

Direct Observation of a Small-Molecule Associated Supramolecular Pigment, Commelinin, by Electrospray Ionization Mass Spectroscopy

1994 ◽  
Vol 116 (16) ◽  
pp. 7457-7458 ◽  
Author(s):  
Tadao Kondo ◽  
Minoru Ueda ◽  
Kumi Yoshida ◽  
Koiti Titani ◽  
Minoru Isobe ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Small Molecule ◽  
Direct Observation ◽  
Mass Spectroscopy ◽  
Ionization Mass ◽  
Electrospray Ionization Mass Spectroscopy ◽  
Ionization Mass Spectroscopy

Mammalian ovarian lipid distributions by desorption electrospray ionization–mass spectrometry (DESI-MS) imaging

2020 ◽  
Vol 412 (6) ◽  
pp. 1251-1262 ◽  
Author(s):  
Fernanda Bertuccez Cordeiro ◽  
Alan K. Jarmusch ◽  
Marisol León ◽  
Christina Ramires Ferreira ◽  
Valentina Pirro ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Ms Imaging ◽  
Desi Ms
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