scholarly journals An adenoviral vector containing an arg–gly–asp (RGD) motif in the fiber knob enhances protein product levels from transgenes refractory to expression

2003 ◽  
Vol 10 (7) ◽  
pp. 559-570 ◽  
Author(s):  
Mel Campbell ◽  
Shimian Qu ◽  
Sam Wells ◽  
Herlina Sugandha ◽  
Roy A Jensen
Keyword(s):  
Adenoviral Vector ◽  
Protein Product ◽  
Rgd Motif

Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

1980 ◽  
Vol 38 ◽  
pp. 540-541
Author(s):  
Dwight Anderson ◽  
Charlene Peterson ◽  
Gursaran Notani ◽  
Bernard Reilly
Keyword(s):  
Electron Microscopy ◽  
Bacillus Subtilis ◽  
Dna Synthesis ◽  
Restriction Endonuclease ◽  
Protein Complex ◽  
Protein Product ◽  
Viral Dna ◽  
Small Proteins ◽  
Antibody Labeling ◽  
Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

Gene Transfer to the Inner Ear Following Adenoviral Vector Inoculation into the Endolymphatic Sac.

2000 ◽  
Vol 59 (1) ◽  
pp. 45-51
Author(s):  
Tatsuya Yamasoba ◽  
Masao Yagi ◽  
Mitsuya Suzuki
Keyword(s):  
Gene Transfer ◽  
Inner Ear ◽  
Adenoviral Vector ◽  
Endolymphatic Sac
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