Nitrile hydratase from a thermophilic Bacillus smithii

1998 ◽  
Vol 20 (3-4) ◽  
pp. 220-226 ◽  
Author(s):  
Y Takashima ◽  
Y Yamaga ◽  
S Mitsuda
Keyword(s):  
Nitrile Hydratase ◽  
Thermophilic Bacillus ◽  
Bacillus Smithii

Crystal structure of nitrile hydratase from a thermophilic Bacillus smithii

2003 ◽  
Vol 312 (2) ◽  
pp. 340-345 ◽  
Author(s):  
Shinji Hourai ◽  
Misao Miki ◽  
Yoshiki Takashima ◽  
Satoshi Mitsuda ◽  
Kazunori Yanagi
Keyword(s):  
Crystal Structure ◽  
Nitrile Hydratase ◽  
Thermophilic Bacillus ◽  
Bacillus Smithii

scholarly journals Establishment of markerless gene deletion tools in thermophilic Bacillus smithii and construction of multiple mutant strains

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Elleke F Bosma ◽  
Antonius H P van de Weijer ◽  
Laurens van der Vlist ◽  
Willem M de Vos ◽  
John van der Oost ◽  
...  
Keyword(s):  
Gene Deletion ◽  
Thermophilic Bacillus ◽  
Mutant Strains ◽  
Bacillus Smithii

Sequence of the gene encoding an alkaline serine protease of thermophilic Bacillus smithii

Gene ◽  
1994 ◽  
Vol 145 (1) ◽  
pp. 149-150 ◽  
Author(s):  
Anna Milano ◽  
Pier Luigi Manachini ◽  
Carlo Parini ◽  
Giovanna Riccardi
Keyword(s):  
Serine Protease ◽  
Gene Encoding ◽  
Alkaline Serine Protease ◽  
Thermophilic Bacillus ◽  
Bacillus Smithii

scholarly journals Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216T

2016 ◽  
Vol 11 (1) ◽  
Author(s):  
Elleke F. Bosma ◽  
Jasper J. Koehorst ◽  
Sacha A. F. T. van Hijum ◽  
Bernadet Renckens ◽  
Bastienne Vriesendorp ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain ◽  
Thermophilic Bacillus ◽  
Bacillus Smithii

Exemplar Abstract for Bacillus smithii Nakamura et al. 1988.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Bacillus Smithii

Insight into the Maturation Process of the Nitrile Hydratase Active Site

2021 ◽  
Author(s):  
Irene R. A. M. Ogutu ◽  
Richard C. Holz ◽  
Brian Bennett
Keyword(s):  
Active Site ◽  
Nitrile Hydratase ◽  
Maturation Process ◽  
Insight Into

The Stability Enhancement of Nitrile Hydratase from Bordetella petrii by Swapping the C-terminal Domain of β subunit

2015 ◽  
Vol 178 (8) ◽  
pp. 1481-1487 ◽  
Author(s):  
Weifeng Sun ◽  
Longbao Zhu ◽  
Xianggui Chen ◽  
Lunjie Wu ◽  
Zhemin Zhou ◽  
...  
Keyword(s):  
Nitrile Hydratase ◽  
Β Subunit ◽  
Terminal Domain ◽  
The Stability ◽  
Stability Enhancement

scholarly journals Transcriptional Regulation of the Nitrile Hydratase Gene Cluster in Pseudomonas chlororaphis B23

2008 ◽  
Vol 190 (12) ◽  
pp. 4210-4217 ◽  
Author(s):  
Toshihide Sakashita ◽  
Yoshiteru Hashimoto ◽  
Ken-Ichi Oinuma ◽  
Michihiko Kobayashi
Keyword(s):  
Gene Cluster ◽  
Nitrile Hydratase ◽  
Transcriptional Regulator ◽  
Pseudomonas Chlororaphis ◽  
Reading Frame ◽  
Transcription Start Sites ◽  
Reverse Transcription Pcr ◽  
Enormous Amount ◽  
Extension Analysis ◽  
Arac Family

ABSTRACT An enormous amount of nitrile hydratase (NHase) is inducibly produced by Pseudomonas chlororaphis B23 after addition of methacrylamide as the sole nitrogen source to a medium. The expression pattern of the P. chlororaphis B23 NHase gene cluster in response to addition of methacrylamide to the medium was investigated. Recently, we reported that the NHase gene cluster comprises seven genes (oxdA, amiA, nhpA, nhpB, nhpC, nhpS, and acsA). Sequence analysis of the 1.5-kb region upstream of the oxdA gene revealed the presence of a 936-bp open reading frame (designated nhpR), which should encode a protein with a molecular mass of 35,098. The deduced amino acid sequence of the nhpR product showed similarity to the sequences of transcriptional regulators belonging to the XylS/AraC family. Although the transcription of the eight genes (nhpR, oxdA, amiA, nhpABC, nhpS, and acsA) in the NHase gene cluster was induced significantly in the P. chlororaphis B23 wild-type strain after addition of methacrylamide to the medium, transcription of these genes in the nhpR disruptant was not induced, demonstrating that nhpR codes for a positive transcriptional regulator in the NHase gene cluster. A reverse transcription-PCR experiment revealed that five genes (oxdA, amiA, nhpA, nhpB, and nhpC) are cotranscribed, as are two other genes (nhpS and acsA). The transcription start sites for nhpR, oxdA, nhpA, and nhpS were mapped by primer extension analysis, and putative −12 and −24 σ54-type promoter binding sites were identified. NhpR was found to be the first transcriptional regulator of NHase belonging to the XylS/AraC family.

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