scholarly journals Hemoglobin consumption by P. falciparum in individual erythrocytes imaged via quantitative phase spectroscopy

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Matthew T. Rinehart ◽  
Han Sang Park ◽  
Katelyn A. Walzer ◽  
Jen-Tsan Ashley Chi ◽  
Adam Wax





The Analyst ◽  
2021 ◽  
Author(s):  
Soorya Pradeep ◽  
Tasmia Tasnim ◽  
Huanan Zhang ◽  
Thomas A. Zangle

Quantitative phase imaging (QPI) used to quantify the mass of soma (cell bodies) and neurites as well as the rates of biomass production due to neurite maturation and formation during neural differentiation.



Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 590
Author(s):  
Jennifer Cauzzo ◽  
Nikhil Jayakumar ◽  
Balpreet Singh Ahluwalia ◽  
Azeem Ahmad ◽  
Nataša Škalko-Basnet

The rapid development of nanomedicine and drug delivery systems calls for new and effective characterization techniques that can accurately characterize both the properties and the behavior of nanosystems. Standard methods such as dynamic light scattering (DLS) and fluorescent-based assays present challenges in terms of system’s instability, machine sensitivity, and loss of tracking ability, among others. In this study, we explore some of the downsides of batch-mode analyses and fluorescent labeling, while introducing quantitative phase microscopy (QPM) as a label-free complimentary characterization technique. Liposomes were used as a model nanocarrier for their therapeutic relevance and structural versatility. A successful immobilization of liposomes in a non-dried setup allowed for static imaging conditions in an off-axis phase microscope. Image reconstruction was then performed with a phase-shifting algorithm providing high spatial resolution. Our results show the potential of QPM to localize subdiffraction-limited liposomes, estimate their size, and track their integrity over time. Moreover, QPM full-field-of-view images enable the estimation of a single-particle-based size distribution, providing an alternative to the batch mode approach. QPM thus overcomes some of the drawbacks of the conventional methods, serving as a relevant complimentary technique in the characterization of nanosystems.



Author(s):  
Sudheesh K Rajput ◽  
Osamu Matoba ◽  
Manoj Kumar ◽  
Xiangyu Quan ◽  
Yasuhiro Awatsuji ◽  
...  


2017 ◽  
Vol 4 (9) ◽  
pp. 1700132 ◽  
Author(s):  
Adam A. L. Michalchuk ◽  
Ivan A. Tumanov ◽  
Sumit Konar ◽  
Simon A. J. Kimber ◽  
Colin R. Pulham ◽  
...  


2017 ◽  
Vol 8 (10) ◽  
pp. 4687 ◽  
Author(s):  
Jiaji Li ◽  
Qian Chen ◽  
Jialin Zhang ◽  
Yan Zhang ◽  
Linpeng Lu ◽  
...  


2007 ◽  
Vol 90 (4) ◽  
pp. 041104 ◽  
Author(s):  
L. Miccio ◽  
D. Alfieri ◽  
S. Grilli ◽  
P. Ferraro ◽  
A. Finizio ◽  
...  




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