scholarly journals A new strategy to measure intercellular adhesion forces in mature cell-cell contacts

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ana Sancho ◽  
Ine Vandersmissen ◽  
Sander Craps ◽  
Aernout Luttun ◽  
Jürgen Groll
Keyword(s):  
Intercellular Adhesion ◽  
Adhesion Forces ◽  
Mature Cell ◽  
Cell Contacts ◽  
New Strategy ◽  
Cell Cell

scholarly journals Asymmetric adhesion of rod-shaped bacteria controls microcolony morphogenesis

2017 ◽  
Author(s):  
Duvernoy Marie-Cécilia ◽  
Mora Thierry ◽  
Ardré Maxime ◽  
Croquette Vincent ◽  
Bensimon David ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Time Lapse ◽  
Adhesion Forces ◽  
Time Resolved ◽  
Cell Contacts ◽  
E Coli ◽  
Daughter Cells ◽  
Substrate Adhesion ◽  
Asymmetrically Distributed ◽  
Cell Cell

Bacterial biofilms are spatially structured communities, within which bacteria can differentiate depending on environmental conditions. During biofilm formation, bacteria attach to a surface and use cell-cell contacts to convey the signals required for the coordination of biofilm morphogenesis. How bacteria can maintain both substrate adhesions and cell-cell contacts during the expansion of a microcolony is still a critical yet poorly understood phenomenon. Here, we describe the development of time-resolved methods to measure substrate adhesion at the single cell level during the formation of E. coli and P. aeruginosa microcolonies. We show that bacterial adhesion is asymmetrically distributed along the cell body. Higher adhesion forces at old poles put the daughter cells under tension and force them to slide along each other. These rearrangements increase cell-cell contacts and the circularity of the colony. We propose a mechanical model based on the microscopic details of adhesive links, which recapitulates microcolony morphogenesis and quantitatively predicts bacterial adhesion from simple time lapse movies. These results explain how the distribution of adhesion forces at the subcellular level directs the shape of bacterial colonies, which ultimately dictates the circulation of secreted signals.

scholarly journals Talin is cut out for intercellular adhesion

2012 ◽  
Vol 197 (6) ◽  
pp. 693-693
Author(s):  
Ben Short
Keyword(s):  
Focal Adhesion ◽  
Intercellular Adhesion ◽  
Cell Contacts ◽  
Cell Cell

Study reveals that arginylation and proteolysis regulate this focal adhesion protein's function at cell–cell contacts.

scholarly journals Molecular cloning of CD31, a putative intercellular adhesion molecule closely related to carcinoembryonic antigen.

1990 ◽  
Vol 171 (6) ◽  
pp. 2147-2152 ◽  
Author(s):  
D L Simmons ◽  
C Walker ◽  
C Power ◽  
R Pigott
Keyword(s):  
Endothelial Cells ◽  
Carcinoembryonic Antigen ◽  
Adhesion Molecule ◽  
Umbilical Vein ◽  
Sequence Similarity ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Cdna Clones ◽  
Cell Contacts ◽  
Cell Cell

cDNA clones encoding CD31 have been isolated by transient expression. The sequence of CD31 expressed on human umbilical vein endothelial cells (HUVEC) is identical to that expressed on the monocyte-like cell line HL60. In HUVEC. CD31 is concentrated in regions of cell-cell contacts. CD31 is a member of the Ig superfamily and is most closely related to the carcinoembryonic antigen CEA, consisting of four contiguous C2 domains. The localization of CD31 to regions of cell-cell contacts, and the sequence similarity to CEA, a known intercellular adhesion molecule (ICAM), strongly suggest that CD31 may function as an ICAM, possibly mediating endothelial cell-cell contacts and also promoting interactions between leukocytes and endothelial cells.

Characterization of N-cadherin-containing cell-cell contacts of the liver

2006 ◽  
Vol 44 (01) ◽  
Author(s):  
BK Straub ◽  
J Boda-Heggemann ◽  
UF Pape ◽  
C Grund ◽  
E Specht-Delius ◽  
...  
Keyword(s):  
Cell Contacts ◽  
Cell Cell
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