α-Chymotrypsin-catalysed peptide synthesis via the kinetically controlled approach using activated esters as acyl donors in organic solvents with low water content: incorporation of non-protein amino acids into peptides

2001 ◽  
pp. 82-86 ◽  
Author(s):  
Toshifumi Miyazawa ◽  
Shin’ichi Nakajo ◽  
Miyako Nishikawa ◽  
Kazumi Hamahara ◽  
Kiwamu Imagawa ◽  
...  
Keyword(s):  
Amino Acids ◽  
Water Content ◽  
Organic Solvents ◽  
Peptide Synthesis ◽  
Activated Esters ◽  
Kinetically Controlled ◽  
Protein Amino Acids ◽  
Acyl Donors ◽  
Kinetically Controlled Approach

Enzymatic peptide synthesis using activated esters as acyl donors in organic media

2006 ◽  
pp. 514-515
Author(s):  
T. Miyazawa ◽  
E. Ensatsu ◽  
K. Tanaka ◽  
R. Yanagihara ◽  
T. Yamada
Keyword(s):  
Peptide Synthesis ◽  
Organic Media ◽  
Enzymatic Peptide Synthesis ◽  
Activated Esters ◽  
Acyl Donors

V8 proteinase-catalyzed peptide synthesis in hydrophilic organic solvents with low water content

1991 ◽  
Vol 32 (28) ◽  
pp. 3421-3424 ◽  
Author(s):  
Václav Cer̆vský
Keyword(s):  
Water Content ◽  
Organic Solvents ◽  
Peptide Synthesis

Utilization of Proteases fromAspergillusSpecies for Peptide Synthesis via the Kinetically Controlled Approach

2003 ◽  
Vol 21 (3) ◽  
pp. 93-100 ◽  
Author(s):  
Toshifumi Miyazawa ◽  
Makoto Hiramatsu ◽  
Takashi Murashima ◽  
Takashi Yamada
Keyword(s):  
Peptide Synthesis ◽  
Kinetically Controlled ◽  
Kinetically Controlled Approach

Preparation of ketimine derivatives of amino acids for peptide synthesis

1974 ◽  
Vol 27 (9) ◽  
pp. 2047 ◽  
Author(s):  
B Halpern ◽  
AP Hope
Keyword(s):  
Amino Acids ◽  
Hydrogen Bond ◽  
Acid Hydrolysis ◽  
Peptide Synthesis ◽  
Room Temperature ◽  
Mild Conditions ◽  
Protein Amino Acids ◽  
Intramolecular Hydrogen ◽  
Derivatives Of ◽  
Strong Intramolecular Hydrogen Bond

Condensates of some 2-hydroxyaryl ketones with protein amino acids have been prepared. The reaction has been shown to proceed without racemization and the derivatives have been isolated as free acids. Their unusual stability is ascribed to the presence of a strong intramolecular hydrogen bond. The N-arylmethylene function is cleaved under mild conditions of acid hydrolysis. Ketimines derived from D-phenylglycine decarboxylate at room temperature.

Semispecific Ester Substrates of Trypsin as Acyl Donors in Kinetically Controlled Peptide Synthesis

1996 ◽  
Vol 14 (3) ◽  
pp. 195-204 ◽  
Author(s):  
I. P. Ivanov ◽  
V. M. Yomtova ◽  
D. D. Petkov
Keyword(s):  
Peptide Synthesis ◽  
Kinetically Controlled ◽  
Acyl Donors

ChemInform Abstract: Lipase-Catalyzed Peptide Synthesis Using Z-Amino Acid Esters as Acyl Donors in Aqueous Water-Miscible Organic Solvents.

ChemInform ◽  
2010 ◽  
Vol 24 (39) ◽  
pp. no-no
Author(s):  
K. KAWASHIRO ◽  
K. KAISO ◽  
D. MINATO ◽  
S. SUGIYAMA ◽  
H. HAYASHI
Keyword(s):  
Amino Acid ◽  
Organic Solvents ◽  
Peptide Synthesis ◽  
Amino Acid Esters ◽  
Acyl Donors ◽  
Acid Esters

α-Chymotrypsin-catalysed peptide synthesis using activated esters as acyl donors

1996 ◽  
pp. 2867-2868 ◽  
Author(s):  
Toshifumi Miyazawa ◽  
Shin'ichi Nakajo ◽  
Miyako Nishikawa ◽  
Kiwamu Imagawa ◽  
Ryoji Yanagihara ◽  
...  
Keyword(s):  
Peptide Synthesis ◽  
Activated Esters ◽  
Acyl Donors

scholarly journals Low-Temperature Stress Tolerance of Grapevine Varieties of Different Ecological and Geographical Origin

2019 ◽  
Vol 73 (1) ◽  
pp. 56-65
Author(s):  
Nataliya I. Nenko ◽  
Irina A. Ilyina ◽  
Galina K. Kiseleva ◽  
Elena K. Yablonskaya
Keyword(s):  
Amino Acids ◽  
Water Content ◽  
Krebs Cycle ◽  
Gravimetric Method ◽  
Stress Factors ◽  
Winter Period ◽  
Climate Conditions ◽  
Abiotic Stress Factors ◽  
Complex Approach ◽  
Protein Amino Acids

Abstract The cold-tolerance of ‘Kristall’ (Euro-Amur-American origin), ‘Dostoyniy’ and ‘Krasnostop AZOS’ (interspecific hybrids of Euro-American origin) grapevine varieties in the winter conditions of South Russia is characterised by the second (true dormancy) and third (induced dormancy) winter-resistance components. Here we used a complex approach to estimate plant adaptation to abiotic stress factors. We used the gravimetric method for humidity assessment and water content, spectral analysis for detection of the protein level and pigments and capillary electrophoresis to determine the level of carbohydrates, Krebs cycle organic acids, phenolcarbonic, ascorbic acids, and amino acids. The obtained results allow to suggest various mechanisms of adaptation of the studied varieties to the winter period stressors. The water content of shoots, levels of protein, amino acids, proline, sugars and the sum of phenolcarbonic acid and ascorbic acid are the most informative indicators of grape plants frost-resistance in the climate conditions of the Anapa–Taman region.

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