scholarly journals Combinatorial fluorescence energy transfer molecular beacons for probing nucleic acid sequences

2006 ◽  
Vol 5 (10) ◽  
pp. 896 ◽  
Author(s):  
Xiaoxu Li ◽  
Zengmin Li ◽  
Angel A. Mart? ◽  
Steffen Jockusch ◽  
Nathan Stevens ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Nucleic Acid ◽  
Molecular Beacons ◽  
Fluorescence Energy Transfer ◽  
Fluorescence Energy ◽  
Nucleic Acid Sequences

Fluorescence Energy Transfer between Fluorescein Label and DNA Intercalators to Detect Nucleic Acids Hybridization in Homogeneous Media

2003 ◽  
Vol 57 (2) ◽  
pp. 208-215 ◽  
Author(s):  
Eva M. Talavera ◽  
Ruperto Bermejo ◽  
Luis Crovetto ◽  
Angel Orte ◽  
Jose M. Alvarez-Pez
Keyword(s):  
Energy Transfer ◽  
Steady State ◽  
Nucleic Acid ◽  
Fluorescence Intensity ◽  
Single Strand ◽  
Fluorescence Energy Transfer ◽  
Steady State Fluorescence ◽  
Fluorescein Label ◽  
Homogeneous Media ◽  
Fluorescence Energy

A general approach to detecting nucleic acid sequences in homogeneous media by means of steady-state fluorescence measurements is proposed. The methodology combines the use of a fluorescence-labeled single-strand DNA model probe, the complementary single-strand DNA target, and a DNA intercalator. The probe was fluorescein labeled to a spacer arm at the N4 position of the cytosine amino groups in polyribocytidylic acid (5′), poly(C), which acts as a model DNA probe. The complementary strand was polyriboinosinic acid (5′), poly(I), as a model of the target, and the energy transfer acceptor was an intercalator, either ethidium bromide or ethidium homodimer. In previous papers we have shown that the fluorescence intensity of the fluorescein label decreases when labeled poly(C) hybridizes with poly(I), and this fluorescence quenching can be used to detect DNA hybridization or renaturation in homogeneous media. In this paper we demonstrate that fluorescence resonance energy transfer (FRET) between fluorescein labeled to poly(C) and an intercalator agent takes place when single-stranded poly(C) hybridizes with poly(I), and we show how the fluorescence energy transfer further decreases the steady-state fluorescence intensity of the label, thus increasing the detection limit of the method. The main aim of this work was to develop a truly homogeneous detection system for specific nucleic acid hybridization in solution using steady-state fluorescence and FRET, but with the advantage of only having to label the probe with the energy donor since the energy acceptor is intercalated spontaneously. Moreover, the site label is not critical and can be labeled randomly in the DNA strand. Thus, the method is simpler than those published previously based on FRET. The experiments were carried out in both direct and competitive formats.

scholarly journals Fluorescence energy transfer as a probe for nucleic acid structures and sequences

1994 ◽  
Vol 22 (6) ◽  
pp. 920-928 ◽  
Author(s):  
Jean-Louis Mergny ◽  
Alexandre S. Boutorine ◽  
Thérèse Garestier ◽  
Francis Belloc ◽  
Michel Rougée ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Nucleic Acid ◽  
Fluorescence Energy Transfer ◽  
Nucleic Acid Structures ◽  
Fluorescence Energy

scholarly journals Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

2021 ◽  
Author(s):  
Noemi Bellassai ◽  
Roberta D’Agata ◽  
Giuseppe Spoto
Keyword(s):  
Nucleic Acid ◽  
Structural Properties ◽  
Molecular Beacon ◽  
Dna Origami ◽  
Molecular Beacons ◽  
Conformational Polymorphism ◽  
Functional Systems ◽  
Similarities And Differences ◽  
Nucleic Acid Structures ◽  
Nucleic Acid Sequences

AbstractNucleic acid nanotechnology designs and develops synthetic nucleic acid strands to fabricate nanosized functional systems. Structural properties and the conformational polymorphism of nucleic acid sequences are inherent characteristics that make nucleic acid nanostructures attractive systems in biosensing. This review critically discusses recent advances in biosensing derived from molecular beacon and DNA origami structures. Molecular beacons belong to a conventional class of nucleic acid structures used in biosensing, whereas DNA origami nanostructures are fabricated by fully exploiting possibilities offered by nucleic acid nanotechnology. We present nucleic acid scaffolds divided into conventional hairpin molecular beacons and DNA origami, and discuss some relevant examples by focusing on peculiar aspects exploited in biosensing applications. We also critically evaluate analytical uses of the synthetic nucleic acid structures in biosensing to point out similarities and differences between traditional hairpin nucleic acid sequences and DNA origami. Graphical abstract

Red Laser-Induced Fluorescence Energy Transfer in an Immunosystem

2000 ◽  
Vol 280 (2) ◽  
pp. 272-277 ◽  
Author(s):  
Bernhard Oswald ◽  
Frank Lehmann ◽  
Lydia Simon ◽  
Ewald Terpetschnig ◽  
Otto S. Wolfbeis
Keyword(s):  
Energy Transfer ◽  
Laser Induced Fluorescence ◽  
Fluorescence Energy Transfer ◽  
Fluorescence Energy

scholarly journals Fluorescence energy transfer in quantum dot/azo dye complexes in polymer track membranes

2013 ◽  
Vol 8 (1) ◽  
pp. 452 ◽  
Author(s):  
Yulia A Gromova ◽  
Anna O Orlova ◽  
Vladimir G Maslov ◽  
Anatoly V Fedorov ◽  
Alexander V Baranov
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Azo Dye ◽  
Fluorescence Energy Transfer ◽  
Track Membranes ◽  
Fluorescence Energy
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