Reactive landing of peptide ions on self-assembled monolayer surfaces: an alternative approach for covalent immobilization of peptides on surfaces

2008 ◽  
Vol 10 (11) ◽  
pp. 1512 ◽  
Author(s):  
Peng Wang ◽  
Omar Hadjar ◽  
Paul L. Gassman ◽  
Julia Laskin
Keyword(s):  
Covalent Immobilization ◽  
Peptide Ions ◽  
Self Assembled Monolayer ◽  
Alternative Approach ◽  
Self Assembled ◽  
Reactive Landing

Soft-landing of peptide ions onto self-assembled monolayer surfaces: an overview

2008 ◽  
Vol 10 (8) ◽  
pp. 1079-1090 ◽  
Author(s):  
Julia Laskin ◽  
Peng Wang ◽  
Omar Hadjar
Keyword(s):  
Peptide Ions ◽  
Soft Landing ◽  
Self Assembled Monolayer ◽  
Self Assembled

Energy Transfer of Peptide Ions Colliding with a Self‐Assembled Monolayer Surface. The Influence of Peptide Ion Size

2019 ◽  
Author(s):  
Meng Gu ◽  
Li Yang ◽  
William L Hase ◽  
Jianmin Sun ◽  
Jiaxu Zhang
Keyword(s):  
Energy Transfer ◽  
Peptide Ions ◽  
Self Assembled Monolayer ◽  
Self Assembled ◽  
Ion Size

Covalent Immobilization of β-Galactosidase onto a Gold-Coated Magnetoelastic Transducer via a Self-Assembled Monolayer:  Toward a Magnetoelastic Biosensor

2003 ◽  
Vol 75 (24) ◽  
pp. 6932-6937 ◽  
Author(s):  
J. Christopher Ball ◽  
Libby G. Puckett ◽  
Leonidas G. Bachas
Keyword(s):  
Covalent Immobilization ◽  
Self Assembled Monolayer ◽  
Magnetoelastic Transducer ◽  
Self Assembled

Charge retention by peptide ions soft-landed onto self-assembled monolayer surfaces

2007 ◽  
Vol 265 (2-3) ◽  
pp. 237-243 ◽  
Author(s):  
Julia Laskin ◽  
Peng Wang ◽  
Omar Hadjar ◽  
Jean H. Futrell ◽  
Jormarie Alvarez ◽  
...  
Keyword(s):  
Peptide Ions ◽  
Self Assembled Monolayer ◽  
Charge Retention ◽  
Self Assembled

Selective Detection of Dopamine in the Presence of Ascorbic and Uric Acids through its Covalent Immobilization on Gold Mercaptopropionic Acid Self-assembled Monolayer

2016 ◽  
Vol 29 (1) ◽  
pp. 272-279 ◽  
Author(s):  
Reza Karimi Shervedani ◽  
Sonia Bahrani ◽  
Marzieh Samiei Foroushani ◽  
Fariborz Momenbeik
Keyword(s):  
Covalent Immobilization ◽  
Selective Detection ◽  
Self Assembled Monolayer ◽  
Mercaptopropionic Acid ◽  
Self Assembled

A Novel Technique for Preparation of the Fluorescence Sensor Based on Covalent Immobilization of 1-Aminopyrene

2011 ◽  
Vol 239-242 ◽  
pp. 1442-1447 ◽  
Author(s):  
Shu Zhen Tan ◽  
Pu Ni Zeng ◽  
Zhong Cao ◽  
Jiao Yun Xia ◽  
Wei Li
Keyword(s):  
Optical Sensors ◽  
Au Nanoparticles ◽  
Covalent Immobilization ◽  
Glass Slide ◽  
Self Assembled Monolayer ◽  
Novel Technique ◽  
Ethylcarbodiimide Hydrochloride ◽  
Carboxylic Groups ◽  
Self Assembled ◽  
Indicator Dyes

A novel technique to covalently immobilize indicator dyes with terminal amino groups for preparing optical sensors is investigated. Au nanoparticles are used as bridges and carriers for anchoring indicator dyes on the surface of a quartz glass slide. 1-Aminopyrene (AP) was employed as an example of indicator dyes and covalently immobilized onto the outmost surface of the glass slide. First, the glass slide was functionalized by (3-mercaptopropyl) trimethoxysilane (MPS) to form a thiol-terminated self-assembled monolayer, where Au nanoparticles were strongly anchored via covalent link. Then, 16-mercaptohexadecanoic acid (MHDA) was self-assembled to bring carboxylic groups onto the surfaces of Au nanoparticles. A further activation by using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) converted the carboxylic group into succinimide ester. Finally, the active succinimide ester was reacted with 1-aminopyrene (AP). Thus, AP was covalently immobilized to the glass slide and an AP-immobilized sensor was obtained. The resulting sensor was used to determine rutin based on fluorescence quenching. It showed a linear response toward rutin (R) from 5.0 × 10-7 to 6.0 × 10-4 mol L-1 with a detection limit of 2.0× 10-7 mol L-1. This AP-immobilized sensor has very satisfactory reproducibility, reversibility, rapid response and no dye-leaching.

Chemical Dynamics Simulations of Energy Transfer in Collisions of Protonated Peptide−Ions with a Perfluorinated Alkylthiol Self-Assembled Monolayer Surface

2008 ◽  
Vol 112 (25) ◽  
pp. 9377-9386 ◽  
Author(s):  
Li Yang ◽  
Oleg A. Mazyar ◽  
U. Lourderaj ◽  
Jiangping Wang ◽  
M. T. Rodgers ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Protonated Peptide ◽  
Chemical Dynamics ◽  
Peptide Ions ◽  
Self Assembled Monolayer ◽  
Self Assembled ◽  
Dynamics Simulations

scholarly journals Impedimetric and Plasmonic Sensing of Collagen I Using a Half-Antibody-Supported, Au-Modified, Self-Assembled Monolayer System

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 227
Author(s):  
Marcin Gwiazda ◽  
Sheetal K. Bhardwaj ◽  
Ewa Kijeńska-Gawrońska ◽  
Wojciech Swieszkowski ◽  
Unni Sivasankaran ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Covalent Immobilization ◽  
Electrochemical Immunosensor ◽  
Collagen I ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
Self Assembled Monolayer ◽  
Self Assembled

This research presents an electrochemical immunosensor for collagen I detection using a self-assembled monolayer (SAM) of gold nanoparticles (AuNPs) and covalently immobilized half-reduced monoclonal antibody as a receptor; this allowed for the validation of the collagen I concentration through two different independent methods: electrochemically by Electrochemical Impedance Spectroscopy (EIS), and optically by Surface Plasmon Resonance (SPR). The high unique advantage of the proposed sensor is based on the performance of the stable covalent immobilization of the AuNPs and enzymatically reduced half-IgG collagen I antibodies, which ensured their appropriate orientation onto the sensor’s surface, good stability, and sensitivity properties. The detection of collagen type I was performed in a concentration range from 1 to 5 pg/mL. Moreover, SPR was utilized to confirm the immobilization of the monoclonal half-antibodies and sensing of collagen I versus time. Furthermore, EIS experiments revealed a limit of detection (LOD) of 0.38 pg/mL. The selectivity of the performed immunosensor was confirmed by negligible responses for BSA. The performed approach of the immunosensor is a novel, innovative attempt that enables the detection of collagen I with very high sensitivity in the range of pg/mL, which is significantly lower than the commonly used enzyme-linked immunosorbent assay (ELISA).

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