Covalent Immobilization of β-Galactosidase onto a Gold-Coated Magnetoelastic Transducer via a Self-Assembled Monolayer:  Toward a Magnetoelastic Biosensor

2003 ◽  
Vol 75 (24) ◽  
pp. 6932-6937 ◽  
Author(s):  
J. Christopher Ball ◽  
Libby G. Puckett ◽  
Leonidas G. Bachas
Keyword(s):  
Covalent Immobilization ◽  
Self Assembled Monolayer ◽  
Magnetoelastic Transducer ◽  
Self Assembled

Selective Detection of Dopamine in the Presence of Ascorbic and Uric Acids through its Covalent Immobilization on Gold Mercaptopropionic Acid Self-assembled Monolayer

2016 ◽  
Vol 29 (1) ◽  
pp. 272-279 ◽  
Author(s):  
Reza Karimi Shervedani ◽  
Sonia Bahrani ◽  
Marzieh Samiei Foroushani ◽  
Fariborz Momenbeik
Keyword(s):  
Covalent Immobilization ◽  
Selective Detection ◽  
Self Assembled Monolayer ◽  
Mercaptopropionic Acid ◽  
Self Assembled

A Novel Technique for Preparation of the Fluorescence Sensor Based on Covalent Immobilization of 1-Aminopyrene

2011 ◽  
Vol 239-242 ◽  
pp. 1442-1447 ◽  
Author(s):  
Shu Zhen Tan ◽  
Pu Ni Zeng ◽  
Zhong Cao ◽  
Jiao Yun Xia ◽  
Wei Li
Keyword(s):  
Optical Sensors ◽  
Au Nanoparticles ◽  
Covalent Immobilization ◽  
Glass Slide ◽  
Self Assembled Monolayer ◽  
Novel Technique ◽  
Ethylcarbodiimide Hydrochloride ◽  
Carboxylic Groups ◽  
Self Assembled ◽  
Indicator Dyes

A novel technique to covalently immobilize indicator dyes with terminal amino groups for preparing optical sensors is investigated. Au nanoparticles are used as bridges and carriers for anchoring indicator dyes on the surface of a quartz glass slide. 1-Aminopyrene (AP) was employed as an example of indicator dyes and covalently immobilized onto the outmost surface of the glass slide. First, the glass slide was functionalized by (3-mercaptopropyl) trimethoxysilane (MPS) to form a thiol-terminated self-assembled monolayer, where Au nanoparticles were strongly anchored via covalent link. Then, 16-mercaptohexadecanoic acid (MHDA) was self-assembled to bring carboxylic groups onto the surfaces of Au nanoparticles. A further activation by using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) converted the carboxylic group into succinimide ester. Finally, the active succinimide ester was reacted with 1-aminopyrene (AP). Thus, AP was covalently immobilized to the glass slide and an AP-immobilized sensor was obtained. The resulting sensor was used to determine rutin based on fluorescence quenching. It showed a linear response toward rutin (R) from 5.0 × 10-7 to 6.0 × 10-4 mol L-1 with a detection limit of 2.0× 10-7 mol L-1. This AP-immobilized sensor has very satisfactory reproducibility, reversibility, rapid response and no dye-leaching.

scholarly journals Impedimetric and Plasmonic Sensing of Collagen I Using a Half-Antibody-Supported, Au-Modified, Self-Assembled Monolayer System

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 227
Author(s):  
Marcin Gwiazda ◽  
Sheetal K. Bhardwaj ◽  
Ewa Kijeńska-Gawrońska ◽  
Wojciech Swieszkowski ◽  
Unni Sivasankaran ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Covalent Immobilization ◽  
Electrochemical Immunosensor ◽  
Collagen I ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
Self Assembled Monolayer ◽  
Self Assembled

This research presents an electrochemical immunosensor for collagen I detection using a self-assembled monolayer (SAM) of gold nanoparticles (AuNPs) and covalently immobilized half-reduced monoclonal antibody as a receptor; this allowed for the validation of the collagen I concentration through two different independent methods: electrochemically by Electrochemical Impedance Spectroscopy (EIS), and optically by Surface Plasmon Resonance (SPR). The high unique advantage of the proposed sensor is based on the performance of the stable covalent immobilization of the AuNPs and enzymatically reduced half-IgG collagen I antibodies, which ensured their appropriate orientation onto the sensor’s surface, good stability, and sensitivity properties. The detection of collagen type I was performed in a concentration range from 1 to 5 pg/mL. Moreover, SPR was utilized to confirm the immobilization of the monoclonal half-antibodies and sensing of collagen I versus time. Furthermore, EIS experiments revealed a limit of detection (LOD) of 0.38 pg/mL. The selectivity of the performed immunosensor was confirmed by negligible responses for BSA. The performed approach of the immunosensor is a novel, innovative attempt that enables the detection of collagen I with very high sensitivity in the range of pg/mL, which is significantly lower than the commonly used enzyme-linked immunosorbent assay (ELISA).

Covalent Immobilization of Peptides on Self-Assembled Monolayer Surfaces Using Soft-Landing of Mass-Selected Ions

2007 ◽  
Vol 129 (28) ◽  
pp. 8682-8683 ◽  
Author(s):  
Peng Wang ◽  
Omar Hadjar ◽  
Julia Laskin
Keyword(s):  
Covalent Immobilization ◽  
Soft Landing ◽  
Self Assembled Monolayer ◽  
Self Assembled

Self-Assembled Fluid Phase Floating Membranes with Tuneable Water Interlayers

2019 ◽  
Author(s):  
Luke Clifton ◽  
Nicoló Paracini ◽  
Arwel V. Hughes ◽  
Jeremy H. Lakey ◽  
Nina-Juliane Seinke ◽  
...  
Keyword(s):  
Fluid Phase ◽  
Interlayer Thickness ◽  
Supported Bilayers ◽  
Self Assembled Monolayer ◽  
High Coverage ◽  
Nano Technology ◽  
Surface Distance ◽  
Potential Applications ◽  
Self Assembled ◽  
Coated Surfaces

<p>We present a reliable method for the fabrication of fluid phase unsaturated bilayers which are readily self-assembled on charged self-assembled monolayer (SAM) surfaces producing high coverage floating supported bilayers where the membrane to surface distance could be controlled with nanometer precision. Vesicle fusion was used to deposit the bilayers onto anionic SAM coated surfaces. Upon assembly the bilayer to SAM solution interlayer thickness was 7-10 Å with evidence suggesting that this layer was present due to SAM hydration repulsion of the bilayer from the surface. This distance could be increased using low concentrations of salts which caused the interlayer thickness to enlarge to ~33 Å. Reducing the salt concentration resulted in a return to a shorter bilayer to surface distance. These accessible and controllable membrane models are well suited to a range of potential applications in biophysical studies, bio-sensors and Nano-technology.</p><br>

Enhancement of electron injection into a light-emitting polymer from an aluminum oxide cathode modified by a self-assembled monolayer

2008 ◽  
Vol 93 (10) ◽  
pp. 103305 ◽  
Author(s):  
Yana Vaynzof ◽  
T. Joseph Dennes ◽  
Jeffrey Schwartz ◽  
Antoine Kahn
Keyword(s):  
Aluminum Oxide ◽  
Electron Injection ◽  
Self Assembled Monolayer ◽  
Light Emitting ◽  
Oxide Cathode ◽  
Self Assembled
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