Probing the catalytic activity and heterogeneity of Au-nanoparticles at the single-molecule level

2009 ◽  
Vol 11 (15) ◽  
pp. 2767 ◽  
Author(s):  
Weilin Xu ◽  
Jason S. Kong ◽  
Peng Chen
Keyword(s):  
Catalytic Activity ◽  
Single Molecule ◽  
Au Nanoparticles ◽  
Single Molecule Level

scholarly journals Single-molecule imaging of telomerase reverse transcriptase in human telomerase holoenzyme and minimal RNP complexes

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Robert Alexander Wu ◽  
Yavuz S Dagdas ◽  
S Tunc Yilmaz ◽  
Ahmet Yildiz ◽  
Kathleen Collins
Keyword(s):  
Catalytic Activity ◽  
Reverse Transcriptase ◽  
Single Molecule ◽  
Cell Extract ◽  
Telomerase Reverse Transcriptase ◽  
Single Molecule Imaging ◽  
Purification Method ◽  
Single Molecule Level ◽  
Synthesis Properties ◽  
Human Telomerase

Telomerase synthesizes chromosome-capping telomeric repeats using an active site in telomerase reverse transcriptase (TERT) and an integral RNA subunit template. The fundamental question of whether human telomerase catalytic activity requires cooperation across two TERT subunits remains under debate. In this study, we describe new approaches of subunit labeling for single-molecule imaging, applied to determine the TERT content of complexes assembled in cells or cell extract. Surprisingly, telomerase reconstitutions yielded heterogeneous DNA-bound TERT monomer and dimer complexes in relative amounts that varied with assembly and purification method. Among the complexes, cellular holoenzyme and minimal recombinant enzyme monomeric for TERT had catalytic activity. Dimerization was suppressed by removing a TERT domain linker with atypical sequence bias, which did not inhibit cellular or minimal enzyme assembly or activity. Overall, this work defines human telomerase DNA binding and synthesis properties at single-molecule level and establishes conserved telomerase subunit architecture from single-celled organisms to humans.

A stochastic theoretical approach to study the size-dependent catalytic activity of a metal nanoparticle at the single molecule level

2017 ◽  
Vol 19 (13) ◽  
pp. 8889-8895 ◽  
Author(s):  
Divya Singh ◽  
Srabanti Chaudhury
Keyword(s):  
Catalytic Activity ◽  
Single Molecule ◽  
Time Distribution ◽  
First Passage Time ◽  
Theoretical Method ◽  
Passage Time ◽  
Metal Nanoparticle ◽  
First Passage ◽  
Single Molecule Level ◽  
Size Dependent

We present a theoretical method based on the first passage time distribution formalism to study the size-dependent catalytic activity of metal nanoparticle at the single molecule level.

Size-Dependent Catalytic Activity and Dynamics of Gold Nanoparticles at the Single-Molecule Level

2010 ◽  
Vol 132 (1) ◽  
pp. 138-146 ◽  
Author(s):  
Xiaochun Zhou ◽  
Weilin Xu ◽  
Guokun Liu ◽  
Debashis Panda ◽  
Peng Chen
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Single Molecule ◽  
Single Molecule Level ◽  
Size Dependent

Atomic Force Microscopy (AFM) for Topography and Recognition Imaging at Single Molecule Level

2013 ◽  
pp. 102-112
Author(s):  
Memed Duman ◽  
Andreas Ebner ◽  
Christian Rankl ◽  
Jilin Tang ◽  
Lilia A. Chtcheglova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Single Molecule Level ◽  
Force Microscopy ◽  
Atomic Force ◽  
Recognition Imaging

Curaxin-Induced DNA Topology Alterations Trigger the Distinct Binding Response of CTCF and FACT at the Single-Molecule Level

Biochemistry ◽  
2021 ◽  
Vol 60 (7) ◽  
pp. 494-499
Author(s):  
Ke Lu ◽  
Cuifang Liu ◽  
Yinuo Liu ◽  
Anfeng Luo ◽  
Jun Chen ◽  
...  
Keyword(s):  
Single Molecule ◽  
Dna Topology ◽  
Single Molecule Level

Control of Triplet Blinking Using Cyclooctatetraene to Access the Dynamics of Biomolecules at the Single‐Molecule Level

2021 ◽  
Author(s):  
Jie Xu ◽  
Shuya Fan ◽  
Lei Xu ◽  
Atsushi Maruyama ◽  
Mamoru Fujitsuka ◽  
...  
Keyword(s):  
Single Molecule ◽  
Single Molecule Level

scholarly journals Power-law behavior of transcription factor dynamics at the single-molecule level implies a continuum affinity model

2021 ◽  
Author(s):  
David A Garcia ◽  
Gregory Fettweis ◽  
Diego M Presman ◽  
Ville Paakinaho ◽  
Christopher Jarzynski ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Single Molecule ◽  
Power Law ◽  
Dwell Time ◽  
Specific Binding ◽  
Power Law Distribution ◽  
Single Molecule Level ◽  
Binding Behavior ◽  
Chromatin Template ◽  
Nuclear Domains

Abstract Single-molecule tracking (SMT) allows the study of transcription factor (TF) dynamics in the nucleus, giving important information regarding the diffusion and binding behavior of these proteins in the nuclear environment. Dwell time distributions obtained by SMT for most TFs appear to follow bi-exponential behavior. This has been ascribed to two discrete populations of TFs—one non-specifically bound to chromatin and another specifically bound to target sites, as implied by decades of biochemical studies. However, emerging studies suggest alternate models for dwell-time distributions, indicating the existence of more than two populations of TFs (multi-exponential distribution), or even the absence of discrete states altogether (power-law distribution). Here, we present an analytical pipeline to evaluate which model best explains SMT data. We find that a broad spectrum of TFs (including glucocorticoid receptor, oestrogen receptor, FOXA1, CTCF) follow a power-law distribution of dwell-times, blurring the temporal line between non-specific and specific binding, suggesting that productive binding may involve longer binding events than previously believed. From these observations, we propose a continuum of affinities model to explain TF dynamics, that is consistent with complex interactions of TFs with multiple nuclear domains as well as binding and searching on the chromatin template.

Magnetic Nanoparticles as a Tool for Remote DNA Manipulations at a Single-Molecule Level

2021 ◽  
Vol 13 (12) ◽  
pp. 14458-14469
Author(s):  
Aleksey A. Nikitin ◽  
Anton Yu Yurenya ◽  
Timofei S. Zatsepin ◽  
Ilya O. Aparin ◽  
Vladimir P. Chekhonin ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Single Molecule ◽  
Single Molecule Level
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