Patch clamping on plane glass—fabrication of hourglass aperture and high-yield ion channel recording

Lab on a Chip ◽  
2009 ◽  
Vol 9 (16) ◽  
pp. 2370 ◽  
Author(s):  
Chang-Yu Chen ◽  
Ting-Yuan Tu ◽  
Chang-Hung Chen ◽  
De-Shien Jong ◽  
Andrew M. Wo
Keyword(s):  
Ion Channel ◽  
High Yield ◽  
Patch Clamping

Automated ion channel screening: patch clamping made easy

2007 ◽  
Vol 11 (4) ◽  
pp. 557-565 ◽  
Author(s):  
Cecilia Farre ◽  
Sonja Stoelzle ◽  
Claudia Haarmann ◽  
Michael George ◽  
Andrea Brüggemann ◽  
...  
Keyword(s):  
Ion Channel ◽  
Patch Clamping

Sweet and Salty: Transduction in Taste

Physiology ◽  
1995 ◽  
Vol 10 (4) ◽  
pp. 166-170
Author(s):  
B Lindemann
Keyword(s):  
Ion Channel ◽  
Molecular Cloning ◽  
Taste Receptor ◽  
Signal Pathways ◽  
Channel Activity ◽  
Patch Clamping ◽  
Receptor Cells ◽  
Intracellular Messengers ◽  
Taste Receptor Cells

Signal pathways in taste receptor cells reach from apical events, like binding of a tastant to a receptor, through intracellular processes and basolateral regenerative ion channel activity to the release of transmitter. The responses are now being investigated with patch clamping, monitoring of intracellular messengers including Ca2+, and molecular cloning.

scholarly journals High Yield Subcortical Patch Clamping in Vivo

2016 ◽  
Vol 110 (3) ◽  
pp. 149a
Author(s):  
William Stoy ◽  
Bo Yang ◽  
Thomas Capocasale ◽  
Clarissa Whitmire ◽  
Yi Liew ◽  
...  
Keyword(s):  
High Yield ◽  
Patch Clamping

Patch-clamping of the inner mitochondrial membrane reveals a voltage-dependent ion channel

Nature ◽  
1987 ◽  
Vol 330 (6147) ◽  
pp. 498-500 ◽  
Author(s):  
M. Catia Sorgato ◽  
Bernhard U. Keller ◽  
Walter Stühmer
Keyword(s):  
Ion Channel ◽  
Mitochondrial Membrane ◽  
Patch Clamping ◽  
Inner Mitochondrial Membrane ◽  
Voltage Dependent

scholarly journals Whole-GUV patch-clamping

2016 ◽  
Vol 114 (2) ◽  
pp. 328-333 ◽  
Author(s):  
Matthias Garten ◽  
Lars D. Mosgaard ◽  
Thomas Bornschlögl ◽  
Stéphane Dieudonné ◽  
Patricia Bassereau ◽  
...  
Keyword(s):  
Ion Transport ◽  
Ion Channel ◽  
High Resistance ◽  
Membrane Properties ◽  
Membrane Tension ◽  
Patch Clamping ◽  
Membrane Composition ◽  
Residual Solvent ◽  
Voltage Dependent

Studying how the membrane modulates ion channel and transporter activity is challenging because cells actively regulate membrane properties, whereas existing in vitro systems have limitations, such as residual solvent and unphysiologically high membrane tension. Cell-sized giant unilamellar vesicles (GUVs) would be ideal for in vitro electrophysiology, but efforts to measure the membrane current of intact GUVs have been unsuccessful. In this work, two challenges for obtaining the “whole-GUV” patch-clamp configuration were identified and resolved. First, unless the patch pipette and GUV pressures are precisely matched in the GUV-attached configuration, breaking the patch membrane also ruptures the GUV. Second, GUVs shrink irreversibly because the membrane/glass adhesion creating the high-resistance seal (>1 GΩ) continuously pulls membrane into the pipette. In contrast, for cell-derived giant plasma membrane vesicles (GPMVs), breaking the patch membrane allows the GPMV contents to passivate the pipette surface, thereby dynamically blocking membrane spreading in the whole-GMPV mode. To mimic this dynamic passivation mechanism, beta-casein was encapsulated into GUVs, yielding a stable, high-resistance, whole-GUV configuration for a range of membrane compositions. Specific membrane capacitance measurements confirmed that the membranes were truly solvent-free and that membrane tension could be controlled over a physiological range. Finally, the potential for ion transport studies was tested using the model ion channel, gramicidin, and voltage-clamp fluorometry measurements were performed with a voltage-dependent fluorophore/quencher pair. Whole-GUV patch-clamping allows ion transport and other voltage-dependent processes to be studied while controlling membrane composition, tension, and shape.

scholarly journals Automated Patch Clamping of 384 Cells at Once for Massively Parallel Ion Channel Screening

2014 ◽  
Vol 106 (2) ◽  
pp. 132a
Author(s):  
Andrea Bruggemann ◽  
Claudia Haarmann ◽  
Timo Stengel ◽  
Marius Vogel ◽  
Juergen Steindl ◽  
...  
Keyword(s):  
Ion Channel ◽  
Massively Parallel ◽  
Patch Clamping

scholarly journals Microchip amplifier for in vitro, in vivo, and automated whole cell patch-clamp recording

2015 ◽  
Vol 113 (4) ◽  
pp. 1275-1282 ◽  
Author(s):  
Reid R. Harrison ◽  
Ilya Kolb ◽  
Suhasa B. Kodandaramaiah ◽  
Alexander A. Chubykin ◽  
Aimei Yang ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Ion Channel ◽  
Voltage Clamp ◽  
Single Cells ◽  
Patch Clamping ◽  
Patch Clamp Recording ◽  
Current Clamp ◽  
Low Levels

Patch clamping is a gold-standard electrophysiology technique that has the temporal resolution and signal-to-noise ratio capable of reporting single ion channel currents, as well as electrical activity of excitable single cells. Despite its usefulness and decades of development, the amplifiers required for patch clamping are expensive and bulky. This has limited the scalability and throughput of patch clamping for single-ion channel and single-cell analyses. In this work, we have developed a custom patch-clamp amplifier microchip that can be fabricated using standard commercial silicon processes capable of performing both voltage- and current-clamp measurements. A key innovation is the use of nonlinear feedback elements in the voltage-clamp amplifier circuit to convert measured currents into logarithmically encoded voltages, thereby eliminating the need for large high-valued resistors, a factor that has limited previous attempts at integration. Benchtop characterization of the chip shows low levels of current noise [1.1 pA root mean square (rms) over 5 kHz] during voltage-clamp measurements and low levels of voltage noise (8.2 μV rms over 10 kHz) during current-clamp measurements. We demonstrate the ability of the chip to perform both current- and voltage-clamp measurement in vitro in HEK293FT cells and cultured neurons. We also demonstrate its ability to perform in vivo recordings as part of a robotic patch-clamping system. The performance of the patch-clamp amplifier microchip compares favorably with much larger commercial instrumentation, enabling benchtop commoditization, miniaturization, and scalable patch-clamp instrumentation.

Development of Ion Channel Analysor Employing Pores of Subunit C of ATP Synthase

2000 ◽  
Vol 6 (S2) ◽  
pp. 978-979
Author(s):  
J. E. M. McGeoch
Keyword(s):  
Ion Channels ◽  
Ion Channel ◽  
Atp Synthase ◽  
Normal Level ◽  
Heart Beat ◽  
Pathological State ◽  
Patch Clamping ◽  
Subunit C ◽  
Sensor Device ◽  
Efficient Screening

Many pathological states are related to aberrant functioning of ion channels. Cures for these conditions involve the design of chemicals that will interact with the ion channels or a channel regulator to return their function to the normal level. A notable example of a pathological state associated with ion channel malfunction is the abnormal conductance of inward rectifying potassium channels associated with irregular heart beat. The development of drugs targeting ion channels requires efficient screening of thousands of potential channel ligands. Classically the function of ion channels are assayed by patch clamping which is a fairly elaborate technique and not efficient from the point of screening thousands of ligands. Here is described the development of a compact sensor device where an ion channel is made to function in a lipid bilayer in 50nm holes in silicon or polymer hardware.

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