Electrochemical approach for the specific detection of hepatitis C virus based on site-specific DNA cleavage of BamHI endonuclease

2010 ◽  
Vol 2 (2) ◽  
pp. 135-142 ◽  
Author(s):  
Shuna Liu ◽  
Qian Wang ◽  
Dongxiao Chen ◽  
Juan Jin ◽  
Yaojuan Hu ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Dna Cleavage ◽  
Specific Detection ◽  
Site Specific ◽  
Electrochemical Approach

scholarly journals Specific Detection of Naturally Occurring Hepatitis C Virus Mutants with Resistance to Telaprevir and Boceprevir (Protease Inhibitors) among Treatment-Naive Infected Individuals

2011 ◽  
Vol 50 (2) ◽  
pp. 281-287 ◽  
Author(s):  
S. Fonseca-Coronado ◽  
A. Escobar-Gutierrez ◽  
K. Ruiz-Tovar ◽  
M. Y. Cruz-Rivera ◽  
P. Rivera-Osorio ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Protease Inhibitors ◽  
Specific Detection ◽  
Naturally Occurring ◽  
Treatment Naïve

scholarly journals Recognition of Multiple Classes of Hepatitis C Antibodies Increases Detection Sensitivity in Oral Fluid

2001 ◽  
Vol 8 (6) ◽  
pp. 1267-1270 ◽  
Author(s):  
Jonathan F. Zmuda ◽  
Barbara Wagoneer ◽  
Lance Liotta ◽  
Gordon Whiteley
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Oral Fluid ◽  
Detection Sensitivity ◽  
Secondary Antibody ◽  
Specific Detection ◽  
Serum Samples ◽  
Specificity And Sensitivity ◽  
Paired Serum ◽  
Antibody Cocktail

ABSTRACT Paired serum-oral fluid samples from 127 hepatitis C virus (HCV)-positive and 31 HCV-negative patients were tested for the presence of anti-HCV using the Ortho HCV 3.0 ELISA. Using the immunoglobulin G (IgG)-specific detection antibody provided with the HCV 3.0 ELISA we attained 100% sensitivity and specificity with serum samples; however, sensitivity in oral fluid samples was only 81%. By modifying the HCV 3.0 ELISA to utilize a secondary antibody cocktail that recognizes not only IgG but IgA and IgM as well, we attained 100% specificity and sensitivity with oral fluid samples.

In Vivo Determination of Substrate Specificity of Hepatitis C Virus NS3 Protease: Genetic Assay for Site-Specific Proteolysis

2000 ◽  
Vol 284 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Sung Yun Kim ◽  
Kye Won Park ◽  
Yong Jae Lee ◽  
Sung Hoon Back ◽  
Jae Hwan Goo ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Substrate Specificity ◽  
Site Specific ◽  
Ns3 Protease ◽  
Genetic Assay

scholarly journals Sensitive and specific detection of hepatitis C virus RNA with nested polymerase chain reaction and digoxigenin labeled cDNA probe.

Kanzo ◽  
1991 ◽  
Vol 32 (2) ◽  
pp. 136-140 ◽  
Author(s):  
Kazuaki CHAYAMA ◽  
Hiromitsu KUMADA ◽  
Satoshi SAITOH ◽  
Yasuji ARASE ◽  
Kenji IKEDA ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cdna Probe ◽  
Specific Detection ◽  
Hepatitis C Virus Rna ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Virus Rna ◽  
Polymerase Chain
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