Heterologous production of bisucaberin using a biosynthetic gene cluster cloned from a deep sea metagenome

2012 ◽  
Vol 8 (2) ◽  
pp. 482-485 ◽  
Author(s):  
Masaki J. Fujita ◽  
Nobutada Kimura ◽  
Hisayoshi Yokose ◽  
Masami Otsuka
Keyword(s):  
Gene Cluster ◽  
Deep Sea ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene

scholarly journals Heterologous Biosynthesis and Genomics-Driven Derivatization of Fungal Bioactive Sesterterpenoid Variecolin

2021 ◽  
Author(s):  
Dexiu Yan ◽  
Jemma Arakelyan ◽  
Teng Wan ◽  
Tsz Ki Chan ◽  
Dohyun Ahn ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Anticancer Activity ◽  
Gene Cluster ◽  
Aspergillus Oryzae ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene ◽  
Aspergillus Aculeatus ◽  
Heterologous Biosynthesis

The biosynthetic gene cluster of fungal bioactive sesterterpenoids, variecolin (1) and variecolactone (2), was identified in Aspergillus aculeatus ATCC 16872. Heterologous production of 1 and 2 was achieved in Aspergillus oryzae by expressing the sesterterpene synthase VrcA and the cytochrome P450 VrcB. Intriguingly, the replacement of VrcB with homologous P450s from other fungal terpenoid pathways yielded three new variecolin analogues, one of which exhibited potent anticancer activity comparable to that of 1.

scholarly journals Heterologous Production of Fosfomycin and Identification of the Minimal Biosynthetic Gene Cluster

2006 ◽  
Vol 13 (11) ◽  
pp. 1171-1182 ◽  
Author(s):  
Ryan D. Woodyer ◽  
Zengyi Shao ◽  
Paul M. Thomas ◽  
Neil L. Kelleher ◽  
Joshua A.V. Blodgett ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene

Heterologous Production of Glidobactins/Luminmycins inEscherichia coliNissle Containing the Glidobactin Biosynthetic Gene Cluster fromBurkholderiaDSM7029

ChemBioChem ◽  
2014 ◽  
Vol 15 (15) ◽  
pp. 2221-2224 ◽  
Author(s):  
Xiaoying Bian ◽  
Fan Huang ◽  
Hailong Wang ◽  
Thorsten Klefisch ◽  
Rolf Müller ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene

scholarly journals Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host

Marine Drugs ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 127 ◽  
Author(s):  
Mingwei Shao ◽  
Juying Ma ◽  
Qinglian Li ◽  
Jianhua Ju
Keyword(s):  
Antibacterial Activity ◽  
Gene Cluster ◽  
Deep Sea ◽  
Streptomyces Coelicolor ◽  
Biosynthetic Gene Cluster ◽  
Type I ◽  
Biosynthetic Gene ◽  
Heterologous Host ◽  
Streptomyces Sp ◽  
Enterococcus Gallinarum

Aborycin is a ribosomally synthesized member of the type I lasso peptide natural products. In the present study, aborycin was isolated and identified from the deep-sea-derived microbe Streptomyces sp. SCSIO ZS0098. The aborycin biosynthetic gene cluster (abo) was identified on the basis of genome sequence analyses and then heterologously expressed in Streptomyces coelicolor M1152 to effectively produce aborycin. Aborycin generated in this fashion exhibited moderate antibacterial activity against 13 Staphylococcus aureus strains from various sources with minimum inhibitory concentrations MICs = 8.0~128 µg/mL, against Enterococcus faecalis ATCC 29212 with an MIC = 8.0 µg/mL, and against Bacillus thuringiensis with MIC = 2.0 µg/mL. Additionally, aborycin displayed potent antibacterial activity (MIC = 0.5 µg/mL) against the poultry pathogen Enterococcus gallinarum 5F52C. The reported abo cluster clearly has the potential to provide a means of expanding the repertoire of anti-infective type I lasso peptides.

Discovery and Heterologous Production of Sarubicins and Quinazolinone C-Glycosides with Protecting Activity for Cardiomyocytes

2021 ◽  
Author(s):  
Mingming Yu ◽  
Jianying Luo ◽  
Dan Luo ◽  
Qiang He ◽  
Yijun Yan ◽  
...  
Keyword(s):  
Natural Products ◽  
Gene Cluster ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Heterologous Production ◽  
Biosynthetic Gene ◽  
Pharmaceutical Agents

Glycosylated natural products and their derivatives are important pharmaceutical agents. Genome mining of C-glycosylated metabolites biosynthetic gene cluster (BGC) from a soil-derived Streptomyces led to the isolation of sarubicins A...

Heterologous production of new protease inhibitory peptide marinostatin E

2021 ◽  
Vol 85 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Kohta Unno ◽  
Hiroyuki Nakagawa ◽  
Shinya Kodani
Keyword(s):  
Gene Cluster ◽  
Marine Bacterium ◽  
Expression Vector ◽  
Biosynthetic Gene Cluster ◽  
Tandem Mass ◽  
Heterologous Production ◽  
Biosynthetic Gene ◽  
Inhibitory Peptide ◽  
Chemical Treatments ◽  
Inhibitory Activities

Abstract Bicyclic peptides, marinostatins, are protease inhibitors derived from the marine bacterium Algicola sagamiensis. The biosynthetic gene cluster of marinostatin was previously identified, although no heterologous production was reported. In this report, the biosynthetic gene cluster of marinostatin (mstA and mstB) was cloned into the expression vector pET-41a(+). As a result of the coexpression experiment, a new analogous peptide named marinostatin E was successfully produced using Escherichia coli BL21(DE3). The structure of marinostatin E was determined by a combination of chemical treatments and tandem mass spectrometry experiments. Marinostatin E exhibited inhibitory activities against chymotrypsin and subtilisin with an IC50 of 4.0 and 39.6 μm, respectively.

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